1 research outputs found
Human sulfatase transiently and functionally active expressed in E. coli K12
The recombinant human iduronate 2-sulfate sulfatase (hrIDS) was
transiently and functionally active expressed in E. coli K12. The
enzyme activity (crude extract) at 100 ml and 400 ml oscillated between
0.25 and 10.58 nmol h-1 mg-1. The wide Western-blot peptide profile
suggest that hrIDS is proteolitically processed \u201crandomly\u201d
which agrees with the ultrafiltration assay in which the hrIDS activity
was found in all fractions (<30kDa, 30-100kDa and >100kDa). No
glycation sites were found by computer analysis of the hIDS sequence;
discarding the possibility of marks for glycation and proteolytic
processing