Dental Treatment Approach in Cantho University of Medicine and Pharmacy of Vietnam

The traditional morphological concept to dental care has shown many drawbacks and is not appropriate in many cases. To counteract these shortcomings, a problem-solving approach has been introduced in dental curriculum of Cantho University of Medicine and Pharmacy (CTUMP), Vietnam. This approach should be reflected in dental practice in CTUMP. Objective: To investigate the problem-solving approach to dental care of CTUMP by patterns of tooth extraction, and tooth rehabilitation. Methods: Cross-sectional data on DMF, dental treatments planned, dental treatments delivered from 1549 dental records of patients aged ≥18 of CTUMP were analyzed. Results: The majority of patients were aged 18-29 (929, 60%), classified as professional and skilled workers (1112 subjects, 72%), lived in urban areas (1156 subjects, 75%), and women (932, 60%). The number of teeth eventually receiving dental treatment was lower than the number of teeth indicated for the treatment. On average, each patient had 2 teeth receiving treatment. Tooth restoration was the most common treatment (1390, 70%). Molars were the most treated teeth (842, 43%). Molars showed statistically significant higher chance for restoration and extraction than premolars and anterior teeth (Wilcoxon-signed-ranks test p ≤ 0.017). No statistically significance was found in tooth replacement between premolar and molar regions. The dental treatments aimed to preserve all teeth regardless of dental regions. Tooth replacement may tend to be morphologically based rather than functionally as most prostheses restored the complete dental arch. Conclusions: The approach to dental care in CTUMP tends to be morphologically conservative.DOI: 10.14693/jdi.v22i1.37

Oral health status of adults in Southern Vietnam - a cross-sectional epidemiological study

Contains fulltext : 89929.pdf (publisher's version ) (Open Access)BACKGROUND: Before strategies or protocols for oral health care can be advised at population level, epidemiological information on tooth decay patterns and its effects on oral function are indispensable. The aim of this study was to investigate influences of socio-demographic variables on the prevalence of decayed, missing, filled (DMF) and sound teeth (St) and to determine the relative risk of teeth in different dental regions for D, M, and F, of adults living in urban and rural areas in Southern Vietnam. METHODS: Cross-sectional DMF and St data of 2965 dentate subjects aged 20 to 95 living in urban and rural areas in three provinces were collected by means of a self-administered questionnaire and an oral examination. The sample was stratified by age, gender, residence and province. RESULTS: The percentage of subjects having missing teeth was high for all ages while it was low for subjects with decayed and filled teeth. The mean number of missing teeth increased gradually by age from approximately 1 in each jaw at the age of 20 to 8 at the age of 80. The number of decayed teeth was relative low at all ages, being highest in molars at young ages. The mean number of filled teeth was extremely low at all ages in all dental regions. Every additional year of age gives a significantly lower chance for decay, a higher chance for missing, and a lower chance for filled teeth. Molars had a significantly higher risk for decay, missing and filled than premolars and anterior teeth. Females had significantly higher risk for decayed and filled teeth, and less chance for missing teeth than males. Urban subjects presented lower risk for decay, but approximately 4 times greater chance for having fillings than rural subjects. Low socio-economic status (SES) significantly increased the chance for missing anterior and molar teeth; subjects with high SES had more often fillings. CONCLUSIONS: The majority of adults of Southern Vietnam presented a reduced dentition. The combination of low numbers of filled teeth and relative high numbers of decayed and missing teeth indicates that the main treatment for decay is extraction. Molars are more at risk for being decayed or missing than premolars and anterior teeth

Differentiation of breast cancer stem cells by knockdown of CD44: promising differentiation therapy

<p>Abstract</p> <p>Background</p> <p>Breast cancer stem cells (BCSCs) are the source of breast tumors. Compared with other cancer cells, cancer stem cells show high resistance to both chemotherapy and radiotherapy. Targeting of BCSCs is thus a potentially promising and effective strategy for breast cancer treatment. Differentiation therapy represents one type of cancer stem-cell-targeting therapy, aimed at attacking the stemness of cancer stem cells, thus reducing their chemo- and radioresistance. In a previous study, we showed that down-regulation of CD44 sensitized BCSCs to the anti-tumor agent doxorubicin. This study aimed to determine if CD44 knockdown caused BCSCs to differentiate into breast cancer non-stem cells (non-BCSCs).</p> <p>Methods</p> <p>We isolated a breast cancer cell population (CD44⁺CD24^-cells) from primary cultures of malignant breast tumors. These cells were sorted into four sub-populations based on their expression of CD44 and CD24 surface markers. CD44 knockdown in the BCSC population was achieved using small hairpin RNA lentivirus particles. The differentiated status of CD44 knock-down BCSCs was evaluated on the basis of changes in CD44⁺CD24^-phenotype, tumorigenesis in NOD/SCID mice, and gene expression in relation to renewal status, metastasis, and cell cycle in comparison with BCSCs and non-BCSCs.</p> <p>Results</p> <p>Knockdown of CD44 caused BCSCs to differentiate into non-BCSCs with lower tumorigenic potential, and altered the cell cycle and expression profiles of some stem cell-related genes, making them more similar to those seen in non-BCSCs.</p> <p>Conclusions</p> <p>Knockdown of CD44 is an effective strategy for attacking the stemness of BCSCs, resulting in a loss of stemness and an increase in susceptibility to chemotherapy or radiation. The results of this study highlight a potential new strategy for breast cancer treatment through the targeting of BCSCs.</p

Seizure prediction : ready for a new era

Acknowledgements: The authors acknowledge colleagues in the international seizure prediction group for valuable discussions. L.K. acknowledges funding support from the National Health and Medical Research Council (APP1130468) and the James S. McDonnell Foundation (220020419) and acknowledges the contribution of Dean R. Freestone at the University of Melbourne, Australia, to the creation of Fig. 3.Peer reviewedPostprin

Dental Treatment Approach in Cantho University of Medicine and Pharmacy of Vietnam

<p>The traditional morphological concept to dental care has shown many drawbacks and is not appropriate in many cases. To counteract these shortcomings, a problem-solving approach has been introduced in dental curriculum of Cantho University of Medicine and Pharmacy (CTUMP), Vietnam. This approach should be reflected in dental practice in CTUMP. <strong>Objective: </strong> To investigate the problem-solving approach to dental care of CTUMP by patterns of tooth extraction, and tooth rehabilitation. <strong>Methods: </strong>Cross-sectional data on DMF, dental treatments planned, dental treatments delivered from 1549 dental records of patients aged ≥18 of CTUMP were analyzed.<strong> Results: </strong>The majority of patients were aged 18-29 (929, 60%), classified as professional and skilled workers (1112 subjects, 72%), lived in urban areas (1156 subjects, 75%), and women (932, 60%). The number of teeth eventually receiving dental treatment was lower than the number of teeth indicated for the treatment. On average, each patient had 2 teeth receiving treatment. Tooth restoration was the most common treatment (1390, 70%). Molars were the most treated teeth (842, 43%). Molars showed statistically significant higher chance for restoration and extraction than premolars and anterior teeth (Wilcoxon-signed-ranks test p ≤ 0.017). No statistically significance was found in tooth replacement between premolar and molar regions. The dental treatments aimed to preserve all teeth regardless of dental regions. Tooth replacement may tend to be morphologically based rather than functionally as most prostheses restored the complete dental arch. <strong>Conclusions:</strong> The approach to dental care in CTUMP tends to be morphologically conservative.</p><p>DOI: <span>10.14693/jdi.v22i1.376</span></p

Prevalence of risk factors for non-communicable diseases in the Mekong Delta, Vietnam: results from a STEPS survey

Abstract Background Despite the increasing burden of non-communicable diseases (NCD) in Vietnam, information on the prevalence of preventable risk factors for NCD is restricted to the main urban centres of Ha Noi, and Ho Chi Minh City (HCMC). This population-based survey aimed to describe the prevalence of risk factors for NCD in a rural Vietnamese sample. Methods This survey was conducted using the WHO "STEPwise approach to surveillance of non-communicable diseases" (STEPS) methodology. Participants (n = 1978) were residents of the Mekong Delta region selected by multi-stage sampling. Standardised international protocols were used to measure behavioural risk factors (smoking, alcohol consumption, fruit and vegetable consumption, physical activity), physical characteristics (weight, height, waist and hip circumferences, blood pressure – BP), fasting blood glucose (BG) and total cholesterol (TC). Data were analysed using complex survey analysis methods. Results In this sample, 8.8% of men and 12.6% of women were overweight (body mass index (BMI) ≥ 25 kg/m2) and 2.3% of men and 1.5% of women were obese (BMI ≥ 30 kg/m2). The prevalence of hypertension (systolic BP ≥ 140 mmHg and/or diastolic BP ≥ 90 mmHg, or taking medication for hypertension) was 27.3% for men and 16.2% for women. There were 1.0% of men and 1.1% of women with raised BG (defined as capillary whole BG of at least 6.1 mmol/L). Conclusion We provide the first NCD risk factor profile of people living in the Mekong Delta of Vietnam using standardised methodology. Our findings for this predominantly rural sample differ from previous studies conducted in Ha Noi and HCMC, and suggest that it is inappropriate to generalise findings from the big-city surveys to the other 80% of the population.</p

Phylodynamics of Enterovirus A71-Associated Hand, Foot, and Mouth Disease in Viet Nam

Enterovirus A71 (EV-A71) is a major cause of hand, foot, and mouth disease (HFMD) and is particularly prevalent in parts of Southeast Asia, affecting thousands of children and infants each year. Revealing the evolutionary and epidemiological dynamics of EV-A71 through time and space is central to understanding its outbreak potential. We generated the full genome sequences of 200 EV-A71 strains sampled from various locations in Viet Nam between 2011 and 2013 and used these sequence data to determine the evolutionary history and phylodynamics of EV-A71 in Viet Nam, providing estimates of the effective reproduction number (Re) of the infection through time. In addition, we described the phylogeography of EV-A71 throughout Southeast Asia, documenting patterns of viral gene flow. Accordingly, our analysis reveals that a rapid genogroup switch from C4 to B5 likely took place during 2012 in Viet Nam. We show that the Re of subgenogroup C4 decreased during the time frame of sampling, whereas that of B5 increased and remained >1 at the end of 2013, corresponding to a rise in B5 prevalence. Our study reveals that the subgenogroup B5 virus that emerged into Viet Nam is closely related to variants that were responsible for large epidemics in Malaysia and Taiwan and therefore extends our knowledge regarding its associated area of endemicity. Subgenogroup B5 evidently has the potential to cause more widespread outbreaks across Southeast Asia