Additional file 2 of BMP and activin membrane-bound inhibitor regulate connective tissue growth factor controlling mesothelioma cell proliferation

Additional file 2: Table S3

Additional file 3 of BMP and activin membrane-bound inhibitor regulate connective tissue growth factor controlling mesothelioma cell proliferation

Additional file 3. Unprocessed western blot images for Fig. 1. Unprocessed western blot images for Fig. 2. Unprocessed western blot images for Fig. 3. Unprocessed western blot images for Figure S3. Unprocessed western blot images for Figure S4

Additional file 1 of BMP and activin membrane-bound inhibitor regulate connective tissue growth factor controlling mesothelioma cell proliferation

Additional file 1: Figure S1. Relative CTGF and BAMBI mRNA levels in MM cells knocked down by transfection of CTGF-targeted siRNA. Relative mRNA expression levels of BAMBI in MSTO-211H, NCI-H28, Y-MESO-8D, NCI-H2052 and NCI-H2452 cells transfected with a CTGF-targeted siRNA (20nM) are shown. BAMBI mRNA expression level was downregulated by transfection of CTGF-targeted siRNA in MSTO-211H, NCI-H28, Y-MESO-8D and NCI-H2052 cells, but not in NCI-H2452 cells. Figure S2. Relative BAMBI mRNA levels and cell proliferation rates of cells knocked down by Santa Cruz's BAMBI siRNA pool. A, Relative mRNA expression levels of BAMBI in Y-MESO-14 and Y-MESO-27 cells transfected with a Santa Cruz's BAMBI siRNA pool (20nM). BAMBI mRNA expression level was downregulated by transfection of siRNA in Y-MESO-14 and Y-MESO-27 cells. B, Graphs of proliferation rates as measured by viable cell counting assay. Figure S3. Knockdown of BAMBI does not affect the TGF-β/Smad activation in Y-MESO-27 cells. Western blotting showed p-Smad2 and p-Smad3 levels in Y-MESO-27 cells. Cells were transfected with a BAMBI-targeted siRNA (20 nM) and compared with nontransfected cells and cells transfected with control siRNA. Cells were stimulated by adding exogenous TGF-β1 and compared with cells without exogenous TGF-β1. Figure S4. Expression and subcellular localization of BAMBI protein in mesothelial and MM cell lines. A, Western blots showing BAMBI levels in seven MM cell lines and the mesothelial MeT-5A cell line. BAMBI protein was ubiquitously expressed by all mesothelioma cell lines but only weakly or below detection limits by MeT-5A cells. B, Immunofluorescence staining of Y-MESO-27 and MeT-5A cells showing that BAMBI localizes to the plasma membrane and cytosol. The nuclei were counterstained with DAPI (blue). The white arrows demarcate BAMBI expression in the cell membrane. Scale bars: 10 μm. Table S1. the oligonucleotide sequences of the siRNAs used in the study. Table S2. the oligonucleotide sequences and accession number of primers used in the study