1,615 research outputs found
Situation of Physical Development 4-5 Years old Preschoolers in Hanoi City, Vietnam
In the education system, preschool is the first level of education that lays the foundation for physical, intellectual, emotional, and aesthetic development, and orients the formation of children\u27s personalities; at the same time promoting the learning and development process in the next stages. Recognizing this importance, the Party and State have constantly paid attention to investing in facilities and quality for this level of education. However, the height growth rate of Vietnamese people is still slower than in other countries in the world and the region. Therefore, in addition to proper nutrition, preschools across the country need to develop many methods that have a profound impact on cognition to improve children\u27s physical health. In this study, the author will focus on assessing the status of physical education at 4-5 years old preschool in Hanoi City, Vietnam. On that basis, specific goals are discussed for the research subjects, to contribute to improving the physical health of 4-5-year-old preschool in the coming time in Hanoi city
Assessment of Experimental Results Application of the Physical Exercise System with Instruments for Kids Kindly 4-5 Years Old in Hanoi City, Vietnam
One of the outstanding results of the preschool education sector in Hanoi in the 2016-2020 period is a comprehensive improvement in school size, class, and quality of child care and education, meeting parents\u27 need to send their children, and at the same time mobilize more and more children of school age. However, physical education for preschool children still faces many difficulties and limitations; especially ancillary works, equipment for children\u27s physical training and sports activities is still lacking and have not met the set requirements. Within the framework of this article, the author evaluates the experimental results of the application of a system of physical exercises for preschool children 4-5 years old in Hanoi City, Vietnam. On that basis, specific goals are discussed for the research subjects, to contribute to improving the physical health of 4-5-year-old preschool in the coming time in Hanoi city
Physical Exercise System Structure with Tools for Preschool Children 4-5 Years old Hanoi City
Structure of physical exercise system with equipment for preschool children 4-5 years old Hanoi City: The structure of the physical exercise system with equipment for 4-year-old preschool children in Hanoi City, of 8 groups with 35 exercises with equipment for the age of 4 is (1) Group of movement exercises; (2) Group of stretching exercises; (3) Group of exercises that stimulate height growth; (4) Group of orientation exercises; (5) Group of exercises for upper limb development; (6) Group of exercises for lower limb development; (7) Group of skillful development exercises; (8) Group of mixed exercises. Structure of physical exercise system with equipment for 5-year-old preschool children in Hanoi City, including 35 exercises of 8 groups oriented to physical development of 5-year-old children: (1) Group of movement exercises; (2) Group of stretching exercises; (3) Group of exercises that stimulate height growth; (4) Group of orientation exercises; (5) Group of exercises for upper limb development; (6) Group of exercises for lower limb development; (7) Group of skillful development exercises; (8) Group of mixed exercises
OPTIMIZATIONOFENZYMATIC HYDROLYSIS PROCESS FROM SHRIMP BY-PRODUCT FOR SHRIMP SAUCE PRODUCTION
Shrimp by-product from shrimp processing industry adapted to food safety was hydrolyzed by alcalase and flavourzyme and optimized by response surface methodology. Shrimp by- product was grounded and treated with fixed alcalase 0.2% (4.8AU/kg protein) andflavourzyme loadings (0.1-0.4%), pH (6.0-9.0), temperature (45-650C) and hydrolysis time (5-13h) in order to improve hydrolysis process in shrimp sauce production. At optimal conditions of pH of 7.5, temperature of 590C,flavourzymeloadings of 0.4% (100LAPU/g protein), alcalaseof 0.2%, and hydrolysis time of 8.2h, hydrolysis degree was 36.76% compared to control sample (hydrolysis by HCl 6N at 1000C for 24h). Shrimp hydrolysis solution was mixed with 25% of NaCl before fermentation. After 10 days of fermentation, shrimp sauce had total nitrogen of 13.2g/l, amino nitrogen of 9.625 g/l, NH3 of 2.13g/l. These properties and sensory quality were equivalent to control sample(2.5 months of fermentation by traditional process).
์ธ๊ฐ ์ ๋ ChaC ๋จ๋ฐฑ์ง์ glutathione ๋์ฌ์์ ๊ตฌ์กฐ๋ฐ ๊ธฐ๋ฅ์ด ๋ํ ์ฐ๊ตฌ
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ผ๋ฌธ(๋ฐ์ฌ)--์์ธ๋ํ๊ต ๋ํ์ :์ฝํ๋ํ ์ฝํ๊ณผ,2020. 2. Byung Woo Han.Glutathione (GSH) degradation plays an essential role not only in the GSH homeostasis but also in housekeeping functions, which regulate cell survival especially in cancer cells. Among human GSH degradation enzymes, cytosolic ChaC1 and ChaC2 enzymes act on GSH to form 5-L-oxoproline and Cys-Gly specifically in cytosol and share 60% sequence similarities. Here, I report the crystal structures of ChaC2 in two different conformations and compare structural features with other known ฮณ-glutamylcyclotransferase enzymes. The unique domain-swapped loop of ChaC2 seems to function as a gate to achieve specificity for GSH binding and regulate the constant GSH degradation rate. Intensive structural and biochemical analyses of ChaC2 revealed that Glu74 and Glu83 play crucial roles in directing the assembly of the swapping dimer and in modulating the enzyme activity. Based on the docking study of GSH to ChaC2 and binding assay, I propose a substrate binding mode and catalytic mechanism. I also found that overexpression of ChaC2, but not ChaC2 E74Q or ChaC2 E83Q, significantly promoted breast cancer cell line proliferation, suggesting that the GSH degradation role of ChaC protein plays a role in breast cancer cell growth. My structural and functional analyses of ChaC2 will contribute to the development of modulators in the ChaC family, which could effectively regulate the progression of GSH degradation-related diseases and cancers.Glutathione(GSH)์ ๋ถํด๋ GSH์ ํญ์์ฑ์ ์ ์งํ ๋ฟ๋ง ์๋๋ผ, ์ ์ธํฌ์์ ์ธํฌ ์์กด์ ์ค์ํ ์ญํ ์ ํ๋ค๊ณ ์๋ ค์ ธ ์๋ค. ํนํ GSH ๋ถํด๋ฅผ ๋ด๋นํ๋ ํจ์๋ค ์ค ChaC1๊ณผ ChaC2๋ ๊ณตํต์ ์ผ๋ก GSH๋ฅผ ๋ถํดํ์ฌ 5-L-oxoproline ๊ณผ Cys-Gly ํฉํ์ด๋๋ฅผ ๋ง๋๋ ์ญํ ์ ํ๋๋ฐ, ์๋ก์ ์๋ฏธ๋
ธ์ฐ ์์ด์ 60% ์ ๋ ์ ์ฌํ๋ค๊ณ ์๋ ค์ ธ ์๋ค. ๋ณธ ์ฐ๊ตฌ์์๋ ChaC2์ ๊ฐ๊ฐ ๋ค๋ฅธ ๋ ๊ฐ์ง ํํ์ ๊ฒฐ์ ๊ตฌ์กฐ๋ฅผ ๋ฐํ๊ณ , ์ด ๊ตฌ์กฐ๋ค์ ๊ธฐ์กด์ ์๋ ค์ ธ ์๋ ๋ค๋ฅธ ฮณ-glutamylcyclotransferase ๊ณ์ด์ ํจ์๋ค๊ณผ ๋น๊ตํ์๋ค. ChaC2 ๊ฒฐ์ ๊ตฌ์กฐ์์ ๋ฐ๊ฒฌ๋๋ domain-๊ตํ loop์ GSH์์ ๊ฒฐํฉ ํน์ด์ฑ์ ๋ถ์ฌํ๋ ๋ถ๋ถ์ผ๋ก, GSH ๋ถํด ์๋๋ฅผ ์กฐ์ ํ๋ ๋ฐ ์ค์ํ ์ญํ ์ ํ๋ ๊ฒ์ผ๋ก ๋ณด์ธ๋ค. ์ฌ๋ฌ ์ํํ์ ์ธ ์คํ ๊ฒฐ๊ณผ์ ๋จ๋ฐฑ์ง ๊ตฌ์กฐ ๋ถ์์ ํตํ์ฌ Glu74์ Glu83์ด ์ดํฉ์ฒด์ ํ์ฑ๊ณผ ๋จ๋ฐฑ์ง ํ์ฑ์ ์กฐ์ ์ ์ค์ํ ์ญํ ์ ํ๋ค๋ ๊ฒ์ ์ ์ ์์๊ณ , GSH์ ChaC2 ์ฌ์ด์ docking ์ฐ๊ตฌ์ ๊ฒฐํฉ ์คํ์ ํตํด GSH์ ChaC2์ ์ฌ์ด์ ๊ฒฐํฉ ๋ฐฉ์๊ณผ ChaC2์ ๋ฐ์ ๋ฉ์ปค๋์ฆ์ ์ ์ํ์๋ค. ๋ฟ๋ง ์๋๋ผ, ChaC2์ ๊ณผ๋ฐํ์ด E74Q์ E83Q ๋ณ์ด ๋จ๋ฐฑ์ง๋ค์ ๋นํด ์ ๋ฐฉ์ ์ธํฌ์ ์์กด์ ์ฆ๊ฐ์ํค๋ ์คํ ๊ฒฐ๊ณผ๋ฅผ ํ ๋๋ก ChaC2์ ์ํ GSH์ ๋ถํด๊ฐ ์ ๋ฐฉ์์ ๋ฐ๋ฌ์ ํน์ ์ญํ ์ ํ ๊ฒ์ด๋ผ ์ ์ํ ์ ์์๋ค.
๊ฒฐ๊ณผ์ ์ผ๋ก, ChaC2์ ๊ตฌ์กฐ์ ๊ธฐ๋ฅ์ ๋ํ ๋ณธ ์ฐ๊ตฌ๋ ChaC ๊ณ์ด ๋จ๋ฐฑ์ง๋ค์ ์กฐ์ ํ ์ ์๋ ๋ถ์์ ๊ฐ๋ฐ์ ๊ธฐ์ฌํ๊ณ , ๋ ๋์๊ฐ GSH ๋ถํด์ ๊ด๋ จ๋ ์ง๋ณ๊ณผ ์์ ํจ์จ์ ์ผ๋ก ์กฐ์ ํ ์ ์๋ ์น๋ฃ์ ๊ธฐ๋ฐ์ ์ ๊ณตํ๋ค.Abstract i
Table of Contents ii
List of Tables iv
List of Figures v
Abbreviations vi
I. Introduction ๏ผ
1. Study Background ๏ผ
2. Purpose of Research ๏ผ
II. Materials and methods ๏ผ
1. Bioinformatics analysis ๏ผ
2. Cloning and protein production ๏ผ
2.1. Cloning and protein production of ChaC2 ๏ผ
2.2. Cloning and protein production of ChaC1 10
3. Mutagenesis 13
4. Crystallization 13
4.1. Crystallization of ChaC2 13
4.2. Crystallization of ChaC1 15
5. X-ray diffraction data collection, structure determination 18
6. Preparation of ChaC2-overexpressing cells 20
7. GSH degradation activity of ChaC enzymes by LC-MS 21
8. GSH assays 22
9. Viability assay (MTT assay) 22
10. Colony-forming assay 23
11. Immunoblotting 23
12. Docking study 23
13. Surface plasmon resonance experiment 23
14. Statistical analysis 24
15. Accession numbers 23
III. Results 25
1. ChaC2 structure was determined through rational Met 25
2. The overall structure of ChaC2 adopts the GGCT fold 31
3. Domain-swapping structure of the ChaC2 homodimer. 33
4. Single mutations of E74Q and E83Q induced conformational 36
5. Structural comparison of human ChaC2 and other GGCT 47
6. Purified ChaC proteins have GGCT activity in vitro 51
7. ChaC2 E74Q and ChaC2 E83Q mutation significantly reduced 53
8. ChaC2 overexpression promotes cell proliferation in MCF-7 55
IV. Discussion 58
1. Role of domain-swapped ChaC2 homodimer conformation 58
2. Proposed mechanism of substrate recognition and GSH... 61
3. Correlation of ChaC2, GSH degradation, and breast cancer 68
Reference 70
Abstract in Korean 73
Acknowledgements 74Docto
Evaluation of Resuscitation Cardiopulmonary Training for the New Nurse
This study aimed to define effective cardiopulmonary resuscitation training for new nurses. Nurses who respond to emergency situations are required to have the sensitivity and professional skills to respond effectively. Thus, improving resuscitation knowledge and technique for nurses is critical in order to enhance the quality and safety of patient care. This study used a pretest and posttest research design with convenience sampling; the sample consisted of 30 nurses who worked at the study hospital. As a result, new nursesโ knowledge improved from 7% to 73% after training. The psychomotor development of skills after the cardiopulmonary resuscitation training improved to 57% from 43%. It is recommended that knowledge and practice skills be maintained by training to ensure ongoing competency
Case Study Methods for Teaching Business and Economics Students
Nowadays more and more universities consider to use The case method in improving teaching
method in business subjects. Case studies will prove learners and students with basic knowledge and
background, for instance, about business situations, then it applies theories to a real situation/event
with clear and understandable examples.
From various fields such as medical, education to business, marketing and political science, case
studies are used to apply global values to local companies. Therefore, it not only help learners to
analyze case context but also help them to present many solutions then choose optimal solution
Serological and molecular surveillance of Orientia tsutsugamushi in scrub typhus patients and coinfection of Huaiyangshan banyangvirus
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ผ๋ฌธ (๋ฐ์ฌ) -- ์์ธ๋ํ๊ต ๋ํ์ : ์๊ณผ๋ํ ์๊ณผํ๊ณผ, 2020. 8. ์กฐ๋จํ.1. Introduction: Scrub typhus is a mite-borne infectious disease caused by Orientia tsutsugamushi. Huaiyangshan banyangvirus (formerly Severe fever with thrombocytopenia syndrome virus (SFTSV)) is a tick-borne virus that can cause hemorrhagic fever. Severe fever with thrombocytopenia syndrome (SFTS) is emerging in Eastern Asia, including China, South Korea, and Japan, which are endemic region of scrub typhus. As the two infections overlap epidemiologically and clinically, knowledge on the local prevalence of the vector-borne infections is critical in target-oriented investigation and treatment.2. Methods: To investigate the genotypic diversity of O. tsutsugamushi and potential coinfection with SFTSV in South Korea and Myanmar, 2,328 sera collected from patients with acute febrile illness in South Korea from 2000 to 2003 and 152 sera obtained from suspected patients with scrub typhus in Myanmar during 2018 were examined. I performed serological and molecular diagnosis of scrub typhus and SFTS. Phylogenetic analysis of both pathogens were also performed by sequencing of the amplified genes from O. tsutsugamushi and SFTSV.3. Results: Among 2,328 samples from Korean patients, serological analysis detecting specific IgG against SFTSV NP antigen identified 37 positive samples (1.6%). Molecular detection of SFTSV NP gene and O. tsutsugamushi tsa56 gene by PCR using the 37 specimens revealed 9 positive for SFTSV and 14 positive for O. tsutsugamushi, respectively. Sequencing of 14 Orientia-specific PCR products showed the presence of 3 genotypes, including Boryong (1/37, 2.7%), Karp (8/37, 21.6%) and Gilliam (4/37, 10.8%) genotypes. In addition, 4 specimens were positive for both SFTSV NP gene and O. tsutsugamushi tsa56 gene, indicating coinfections. Among 152 Myanmar samples, 9 samples (5.9%) were positive for O. tsutsugamushi tsa56 gene and sequencing of the PCR products revealed the presence of 5 genotypes, Karp A (4/9, 44,4%), Karp B (1/9, 11.1%), Kato B (2/9, 22.2%), Gilliam (1/9, 11.1%) and JG_C (1/9, 11.1%). 5 samples were positive for SFTSV NP gene-specific PCR (5/152, 3.28%). These 5 SFTSV NP gene positive samples were all negative for O. tsutsugamushi tsa56 gene, but 3 of them carried high titers (โฅ1:2,560) of IgG or IgM or both antibodies specific to O. tsutsugamushi, as measured by indirect immunofluorescence assay.
4. Conclusion: In this study, serological and molecular surveillance of sera collected from suspected scrub typhus patients in South Korea and Myanmar revealed serological prevalence and genotypic heterogeneity of O. tsutsutsugamushi. Moreover, potential coinfection of O. tsutsugamushi and SFTSV was identified by serological and molecular surveillance. These results suggest that SFTSV infections might have been spread more widely in Eastern Asia. Therefore, continuous surveillance of SFTS patients needs to be conducted in the local area. In addition, more reliable differential diagnosis techniques and prevention and control measures are required for better clinical practices and outcomes in the endemic regions of multiple tick-borne and mite-borne pathogens.INTRODUCTION 7
MATERIALS AND METHODS 16
RESULTS 23
DISCUSSION 54
REFERENCES 63
ABSTRACT IN KOREAN 73Docto
Legal System Reform and Challenges on Assurance of Human Rights Standards in Vietnam
Since the Doi Moi (Vietnamโs economic reform) in 1986 to date, Vietnam has continuously made efforts to reform its legal system to better adapt with requirements to develop in different historical periods. Vietnam considers ensuring fundamental human rights as one of the cross-cutting goals in national development policies and strategies. It is undeniable that the outcomes of legal system reform had a positive impact on all aspects of life and on the assurance of human rights and citizenโs rights in particular. However, due to the effects of e international integration and 4.0 industrial revolution the emergence of new-generation trade agreements (FTAs), the current legal system in Vietnam has revealed certain limitations that need to be improved in order to better guarantee fundamental rights. With the objective to bring about the general picture of legal reform on human rights in the recent years in Vietnam, this article will focus on introducing the legal system and its impact on the assurance of human rights standards and highlighting the priorities that need to be revised
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