INVESTIGATION IN LOADING 5-FLUOROURACIL ABILITY OF IRON-ORGANIC FRAMEWORKS

Materials MIL-53(Fe), MIL-88(Fe) and MIL-100(Fe) have the ability to absorb many different compounds. In addition, the materials are small in size, highly bio-compatible, with no human toxicity. These materials were chosen to carry 5-fluorouracil (5-FU) for cancer treatment. Synthetic materials were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and surface characteristics (BET). 5-FU loading and releasing ability of MIL-53(Fe), MIL-88(Fe) and MIL-100(Fe) have been investigated by UV-Vis spectrophotometer. The results showed that the MIL-53(Fe), MIL-88(Fe), and MIL-100(Fe) are capable of carrying 5-FU with capacity exceeding 0.131 g/g, 0.28 g/g, 0.66 g/g respectively and mostly released after 10 days. The cancer cell toxicity and slow drug release ability of MIL(Fe)@5-FU were also tested by in-vitro method. Iron-organic frameworks are promising materials for cancer treatment

Cấp cứu ngoại khoa về bụng

138 tr. ; 24 c

Bệnh học ngoại : Tuỵ - Lách : Sau đại học

216 tr. ; 21 c

Phẫu thuật ống tiêu hoá

225 tr. ; 24 cm

Hỏi và đáp về môn học tư tưởng Hồ Chí Minh : Dùng cho sinh viên các hệ đào tạo đại học, cao đẳng

258 tr. ; 21 cm

Pháp luật học

234 tr. ; 24 cm

Cloning and constructing transformation vector carrying IbOr from the sweetpotato (Impomea batatas L.) involved in accumulation of carotenoids

The Orange gene (Or gene) is known as a gene encoding the protein containing a cysteine-rich Zinc finger domain. The Or protein is an important component of the structure that involes in accumulation of carotenoids and enhancing the resistant ability of plant to enviromental stresses. In an attempt to improve the nutritional quality and resistance to adverse environmental conditions of maize, in this article, we present the results on cloning IbOr gene from the sweetpotato cultivar Hoang Long and constructing transformation vectors carrying cloned IbOr gene under control of Ubiquitin (Ubi) or Globulin 1 (Glo1) promoter. The cloned IbOr gene was 942 bp in size and had similarity level of 100% comparing to the IbOr gene that was deposited in GenBank with Accession number HQ828087.1. The IbOr gene from sweedpotato Hoang Long was registered in GenBank under Accession number KX792094.1. The deduced protein consisted of 313 amino acids having Zin finger domain of DnaJ and HSP40 proteins. We have also constructed two transformation vectors pCambia2300/Ubiquitin/IbOr/Nos and pCambia2300/Glo1/IbOr/Nos. These vectors were transformed successfully into Agrobacterium tumefaciens strain C58. The presence of the target gene (IbOr) and promoter (Ubi or Glo1) in the A. tumefaciens strains were confirmed by colony-PCR amplification with respective specific primer pairs and digested by suitable restriction enzymes. These vectors will provide important materials for further gene transfer research for the expression of IbOr gene and production of transgenic maize with ability to accumulate high carotenoids