Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Energy Efficient Lighting Design Using LEDs and Other Technologies (semester?), IPRO 337: Energy Efficient Lighting IPRO 337 Abstract Sp07

• Incorporation of energy efficient lighting technologies into an existing 6,000 sq ft space, thereby adding to the transformation of the space into a new useful zero-energy lab. • Testing and measurement of energy consuming Devices that are scaled for renewable energy systems such as photovoltaic, battery storage, and fuel cells. • Demonstration of the use of day lighting for ambient light. Demonstration of use of low energy consuming systems for task lighting and night time lighting. • Development of low energy consuming lighting systems including fixture mock ups, lamp mock ups, day lighting harvesting systems, fiber optic day light utilization systems, fixtures utilizing LED, low wattage fluorescent and direct and indirect lighting fixture design.Deliverables for IPRO 337: Energy Efficient Lighting Design Using LEDs and Other Technologies for the Spring 2007 semeste

Energy Efficient Lighting Design Using LEDs and Other Technologies (semester?), IPRO 337: Energy Efficient Lighting IPRO 337 IPRO Day Presentation Sp07

• Incorporation of energy efficient lighting technologies into an existing 6,000 sq ft space, thereby adding to the transformation of the space into a new useful zero-energy lab. • Testing and measurement of energy consuming Devices that are scaled for renewable energy systems such as photovoltaic, battery storage, and fuel cells. • Demonstration of the use of day lighting for ambient light. Demonstration of use of low energy consuming systems for task lighting and night time lighting. • Development of low energy consuming lighting systems including fixture mock ups, lamp mock ups, day lighting harvesting systems, fiber optic day light utilization systems, fixtures utilizing LED, low wattage fluorescent and direct and indirect lighting fixture design.Deliverables for IPRO 337: Energy Efficient Lighting Design Using LEDs and Other Technologies for the Spring 2007 semeste

Energy Efficient Lighting Design Using LEDs and Other Technologies (semester?), IPRO 337: Energy Efficient Lighting IPRO 337 Midterm Report Sp07

• Incorporation of energy efficient lighting technologies into an existing 6,000 sq ft space, thereby adding to the transformation of the space into a new useful zero-energy lab. • Testing and measurement of energy consuming Devices that are scaled for renewable energy systems such as photovoltaic, battery storage, and fuel cells. • Demonstration of the use of day lighting for ambient light. Demonstration of use of low energy consuming systems for task lighting and night time lighting. • Development of low energy consuming lighting systems including fixture mock ups, lamp mock ups, day lighting harvesting systems, fiber optic day light utilization systems, fixtures utilizing LED, low wattage fluorescent and direct and indirect lighting fixture design.Deliverables for IPRO 337: Energy Efficient Lighting Design Using LEDs and Other Technologies for the Spring 2007 semeste

Energy Efficient Lighting Design Using LEDs and Other Technologies (semester?), IPRO 337: Energy Efficient Lighting IPRO 337 Project Plan Sp07

• Incorporation of energy efficient lighting technologies into an existing 6,000 sq ft space, thereby adding to the transformation of the space into a new useful zero-energy lab. • Testing and measurement of energy consuming Devices that are scaled for renewable energy systems such as photovoltaic, battery storage, and fuel cells. • Demonstration of the use of day lighting for ambient light. Demonstration of use of low energy consuming systems for task lighting and night time lighting. • Development of low energy consuming lighting systems including fixture mock ups, lamp mock ups, day lighting harvesting systems, fiber optic day light utilization systems, fixtures utilizing LED, low wattage fluorescent and direct and indirect lighting fixture design.Deliverables for IPRO 337: Energy Efficient Lighting Design Using LEDs and Other Technologies for the Spring 2007 semeste

Energy Efficient Lighting Design Using LEDs and Other Technologies (semester?), IPRO 337

• Incorporation of energy efficient lighting technologies into an existing 6,000 sq ft space, thereby adding to the transformation of the space into a new useful zero-energy lab. • Testing and measurement of energy consuming Devices that are scaled for renewable energy systems such as photovoltaic, battery storage, and fuel cells. • Demonstration of the use of day lighting for ambient light. Demonstration of use of low energy consuming systems for task lighting and night time lighting. • Development of low energy consuming lighting systems including fixture mock ups, lamp mock ups, day lighting harvesting systems, fiber optic day light utilization systems, fixtures utilizing LED, low wattage fluorescent and direct and indirect lighting fixture design.Deliverables for IPRO 337: Energy Efficient Lighting Design Using LEDs and Other Technologies for the Spring 2007 semeste

Identification of a Small Molecule Compound Active against Antibiotic-Tolerant <i>Staphylococcus aureus</i> by Boosting ATP Synthesis

Antibiotic tolerance poses a threat to current antimicrobial armamentarium. Bacteria at a tolerant state survive in the presence of antibiotic treatment and account for persistence, relapse and recalcitrance of infections. Antibiotic treatment failure may occur due to antibiotic tolerance. Persistent infections are difficult to treat and are often associated with poor prognosis, imposing an enormous burden on the healthcare system. Effective strategies targeting antibiotic-tolerant bacteria are therefore highly warranted. In this study, small molecule compound SA-558 was identified to be effective against Staphylococcus aureus that are tolerant to being killed by conventional antibiotics. SA-558 mediated electroneutral transport across the membrane and led to increased ATP and ROS generation, resulting in a reduction of the population of antibiotic-tolerant bacteria. In a murine chronic infection model, of which vancomycin treatment failed, we demonstrated that SA-558 alone and in combination with vancomycin caused significant reduction of MRSA abundance. Our results indicate that SA-558 monotherapy or combinatorial therapy with vancomycin is an option for managing persistent S. aureus bacteremia infection and corroborate that bacterial metabolism is an important target for counteracting antibiotic tolerance

Simeprevir Potently Suppresses SARS-CoV-2 Replication and Synergizes with Remdesivir

International audienc

Simeprevir Potently Suppresses SARS-CoV-2 Replication and Synergizes with Remdesivir

International audienc