2 research outputs found
Exploration of hypoglycemic effect of an extract from leaves of a plant from Tunisian pharmacopeia: Artemisia campestris (Asteraceae)
Background and objectives: A lot of research has been directed towards
medicinal plants which are considered as a source of multiple
phytotherapic substances endowed with hypoglycemic activities that
could be used to treat diabetes and its complications. Our study was
carried out in Wistar rats to investigate the hypoglycemic effect of
n-Butanol Fraction from Artemisia campestris leaf Methanolic Extract
(BFACME). Methods: Two experimental models were used in rats: orally
induced hyperglycemia (OGTT) and isolated perfused liver (IPRL).
Results: BFACME at 550 mg/kg BW dose significantly reduced fasting
glucose level in normal rats as compared to controls. The decrease of
glycaemia was 12.6% more significant than that obtained with the
standard drug glibenclamide (10 mg/kg BW), an oral antidiabetic
preparation belonging to sulfonylurea class. In OGTT model, BFACME at
the highest doses of 550 and 400 mg/kg BW significantly reduced the
postprandial hyperglycemic peak compared to controls. In the IPRL
model, treatment with BFACME significantly decreased glucose
concentrations after 30 min of perfusion with 30 mM glucose solely when
insulin was present. The higher doses of BFACME lead to glucose
concentration at basal level as early as 90 min, while the lowest dose
does not restore this concentration even to t = 120min. The best
initial glucose concentration retrieval was obtained with 0.7 mg
BFACME/mL/g liver. At this dose, BFACME improves the decrease of
glucose level caused by only insulin by about 18%. Conclusion: The
BFACME appears to exert a hypoglycemic activity by potentiating the
insulin action