Electric field-induced structural rearrangements in biomembranes

Neumann E. Electric field-induced structural rearrangements in biomembranes. Electrochimica Acta. 1989;34(12):1905-1907

Thermodynamic investigation of the helix-coil transitions of a polyribonucleotide system

Neumann E, Ackermann T. Thermodynamic investigation of the helix-coil transitions of a polyribonucleotide system. Journal of Physical Chemistry. 1969;73(7):2170-2178

Kinetics and mechanism for the conformational transition in p-guanidinobenzoate bovine trypsinogen induced by the isoleucine-valine dipeptide

Nolte H-J, Neumann E. Kinetics and mechanism for the conformational transition in p-guanidinobenzoate bovine trypsinogen induced by the isoleucine-valine dipeptide. Biophysical Chemistry. 1979;10(3-4):253-260

Experimental thermodynamics of the helix-random coil transition. II. Calorimetric measurement of transition enthalpies of poly A in acid aqueous solution

Klump H, Ackermann T, Neumann E. Experimental thermodynamics of the helix-random coil transition. II. Calorimetric measurement of transition enthalpies of poly A in acid aqueous solution. Biopolymers. 1969;7(3):423-431.The enthalpy change accompanying the double helix-coil transition of polyriboadenylic acid (poly A) in aqueous solution has been measured optically and calorimetrically in the pH range 5.7-4.5. The course of this cooperative transition was followed optically by measuring changes in ultraviolet absorption as a function of temperature at different pH values, and calorimetrically by determining the heat capacity of the solution through the transition region. From the latter measurements, the enthalpy of transition was calculated. It is shown, that H is dependent on pH as it is expected from the influence of protonation of the double helix of poly A

Electrotransformation of intact and osmotically sensitive cells of Corynebacterium glutamicum

Wolf H, Pühler A, Neumann E. Electrotransformation of intact and osmotically sensitive cells of Corynebacterium glutamicum. Applied Microbiology and Biotechnology. 1989;30(3):283-289

Mechanisms of membrane processes, electric gene transfer and cell fusion Report on the workshop held at the Zentrum für Interdisziplinäre Forschung in Bielefeld on 3–4 April 1986

Dorfmüller T, Förster W, Neumann E. Mechanisms of membrane processes, electric gene transfer and cell fusion Report on the workshop held at the Zentrum für Interdisziplinäre Forschung in Bielefeld on 3–4 April 1986. Bioelectrochemistry and Bioenergetics. 1987;17(1):7-8

The nematocyst extract of Hydra attenuata causes single channel events in lipid bilayers

Weber J, Schürholz T, Neumann E. The nematocyst extract of Hydra attenuata causes single channel events in lipid bilayers. Toxicon. 1990;28(4):403-409

Extension of Ahmed & Thompson Theory to General Elastic Plane Quasi-Wave Propagation in Textured Polycrystalline Material

In polycrystalline materials, such as columnar grained austenitic weld metal, grain scattering occurs. The extent of the scattering depends on the elastic anisotropy of the grains and the geometric features of the grains and grain boundaries. Therefore these properties have to be incorporated in any theoretical modelling

Kinetic calibration of the calcium indicator arsenazo III. I. Stopped-flow spectroscopy

Clemen S, Rabl C-R, Neumann E. Kinetic calibration of the calcium indicator arsenazo III. I. Stopped-flow spectroscopy. Biochimica et Biophysica Acta (BBA) - Biomembranes. 1988;938(3):456-460

Control by pulse parameters of electric field-mediated gene transfer in mammalian cells

Wolf H, Rols MP, Boldt E, Neumann E, Teissie J. Control by pulse parameters of electric field-mediated gene transfer in mammalian cells. Biophysical Journal. 1994;66(2):524-531.Electric field-mediated gene transfer in mammalian cells (electrotransformation) depends on the pulsing conditions (field intensity, pulse duration, number of pulses). The effect of these parameters was systematically investigated using the transient expression of the chloramphenicol acetyltransferase and the beta-galactosidase activities in Chinese hamster ovary cells. Pulsing conditions inducing reversible permeabilization of the cell plasma membrane are not sufficient to induce gene transfer. The plasmid must be present during the electric pulse if it is to be transferred across the membrane into the cytoplasm. Only the localized part of the cell membrane brought to the permeabilized state by the external field is competent. Pulse duration plays a key role in the magnitude of the transfer. The field induces a complex reaction between the membrane and the plasmid that is accumulated at the cell interface by electrophoretic forces. This leads to an insertion of the plasmid, which can then cross the membrane