242 research outputs found
Photonic applications of azobenzene molecules embedded in amorphous polymer
AbstractThe incorporation of azobenzene chromophores into polymer systems gives rise to a number of unique effects under UV and visible light irradiation. The light-driven isomerization of the azobenzene element acts as a light-to-mechanical energy converter, translating the nanoscopic structural movement of the isomerization azobenzene into macroscopic topographic film modulation in the form of surface relief. This review focuses on the study of reversible changes in shape in various systems incorporating azobenzene, including large-scale superficial photo-patterned glassy materials, light-driven reshaping of tridimensional superficial azo-textures and contractions of stimuli-responsive liquid crystalline networks (LCNs). Further, promising applications of azo systems are investigated as smart biointerfaces able to mimic time-varying biological systems
A switchable light-responsive azopolymer conjugating protein micropatterns with topography for mechanobiological studies
Stem cell shape and mechanical properties in vitro can be directed by geometrically defined micropatterned adhesion substrates. However conventional methods are limited by the fixed micropattern design, which cannot recapitulate the dynamic changes of the natural cell microenvironment. Current methods to fabricate dynamic platforms usually rely on complex chemical strategies or require specialized apparatuses. Also, with these methods the integration of dynamic signals acting on different length scales is not straightforward, whereas in some applications might be beneficial to act on both a microscale level, i.e. cell shape, and on a nanoscale level, i.e. cell adhesions.
Here, we exploited a confocal laser-based technique on a light-responsive azopolymer displaying micropatterns of adhesive islands. The laser light promotes a directed mass migration and the formation of submicrometric topographic relieves. Also, by changing the surface chemistry, the surfacing topography affects cell spreading and shape. This method enabled us to monitor in a non-invasive manner the dynamic changes in focal adhesions, cytoskeleton structures and nucleus conformation that followed the changes in the adhesive characteristic of the substrate. Focal adhesions reconfigured after the surfacing of the topography and the actin filaments reoriented to co-align with the newly formed adhesive island. Changes in cell morphology also affected nucleus shape, chromatin conformation and cell mechanics with different timescales.
The reported strategy can be used to investigate mechanotransduction-related events dynamically by controlling cell adhesion at a cell shape and focal adhesion levels. The integrated technique enables achieving a submicrometric resolution in a facile and cost-effective manner
Synthesis of semicrystalline nanocapsular structures obtained by Thermally Induced Phase Separation in nanoconfinement
Phase separation of a polymer solution exhibits a peculiar behavior when induced in a nanoconfinement. The energetic constraints introduce additional interactions between the polymer segments that reduce the number of available configurations. In our work, this effect is exploited in a one-step strategy called nanoconfined-Thermally Induced Phase Separation (nc-TIPS) to promote the crystallization of polymer chains into nanocapsular structures of controlled size and shell thickness. This is accomplished by performing a quench step of a low-concentrated PLLA-dioxane-water solution included in emulsions of mean droplet size <500 nm acting as nanodomains. The control of nanoconfinement conditions enables not only the production of nanocapsules with a minimum mean particle diameter of 70 nm but also the tunability of shell thickness and its crystallinity degree. The specific properties of the developed nanocapsular architectures have important implications on release mechanism and loading capability of hydrophilic and lipophilic payload compounds
Hybrid core-shell (HyCoS) nanoparticles produced by complex coacervation for multimodal applications
Multimodal imaging probes can provide diagnostic information combining different imaging modalities. Nanoparticles (NPs) can contain two or more imaging tracers that allow several diagnostic techniques to be used simultaneously. In this work, a complex coacervation process to produce core-shell completely biocompatible polymeric nanoparticles (HyCoS) for multimodal imaging applications is described. Innovations on the traditional coacervation process are found in the control of the reaction temperature, allowing a speeding up of the reaction itself, and the production of a double-crosslinked system to improve the stability of the nanostructures in the presence of a clinically relevant contrast agent for MRI (Gd-DTPA). Through the control of the crosslinking behavior, an increase up to 6 times of the relaxometric properties of the Gd-DTPA is achieved. Furthermore, HyCoS can be loaded with a high amount of dye such as ATTO 633 or conjugated with a model dye such as FITC for in vivo optical imaging. The results show stable core-shell polymeric nanoparticles that can be used both for MRI and for optical applications allowing detection free from harmful radiation. Additionally, preliminary results about the possibility to trigger the release of a drug through a pH effect are reported
Encoding multiple holograms for speckle-noise reduction in optical display.
In digital holography (DH) a mixture of speckle and incoherent additive noise, which appears in numerical as well as in optical reconstruction, typically degrades the information of the object wavefront. Several methods have been proposed in order to suppress the noise contributions during recording or even during the reconstruction steps. Many of them are based on the incoherent combination of multiple holographic reconstructions achieving remarkable improvement, but only in the numerical reconstruction i.e. visualization on a pc monitor. So far, it has not been shown the direct synthesis of a digital hologram which provides the denoised optical reconstruction. Here, we propose a new effective method for encoding in a single complex wavefront the contribution of multiple incoherent reconstructions, thus allowing to obtain a single synthetic digital hologram that show significant speckle-reduction when optically projected by a Spatial Light Modulator (SLM)
distribution and bioactivity of the ret specific d4 aptamer in three dimensional collagen gel cultures
The success of tyrosine kinase inhibitors in cancer therapy prompted intensive research efforts addressed to the development of new specific diagnostics and therapeutics. Targeting large transmembrane molecules, including receptor tyrosine kinases, is a major pharmacologic challenge. The D4 RNA-aptamer, isolated applying the Systematic Evolution of Ligand by Exponential Enrichment procedure on living cells, has been proven a specific inhibitor of the human receptor tyrosine kinase Ret. In our attempts to generate new powerful probes for in vivo applications, in the present study, we addressed the ability of D4 to preserve its biological activity in cells embedded in three-dimensional collagen gels. These matrices provide a microenvironment mimicking the cell organization as seen in vivo , thus representing a suitable tool to approach the use of the aptamer in vivo . By taking advantage of transformed fibroblasts expressing Ret as a model system, we showed that the cells maintain normal phenotype and growth patterns when cultured in three-dimensional matrices and that the D4 aptamer preserves its ability to inhibit Ret on the surface of the cells embedded in collagen. Because the biological activity of RNA aptamers is largely dictated by their folded structure, the results indicate that a folded conformation of D4 responsible of its inhibiting function is preserved in the three-dimensional constructs, thus supporting its use in tumors in vivo . [Mol Cancer Ther 2008;7(10):3381–8
Correction: Rheometry-on-a-chip: measuring the relaxation time of a viscoelastic liquid through particle migration in microchannel flows
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Radiolabeled PET/MRI Nanoparticles for Tumor Imaging
The development of integrated positron emission tomography (PET)/ magnetic resonance imaging (MRI) scanners opened a new scenario for cancer diagnosis, treatment, and follow-up. Multimodal imaging combines functional and morphological information from different modalities, which, singularly, cannot provide a comprehensive pathophysiological overview. Molecular imaging exploits multimodal imaging in order to obtain information at a biological and cellular level; in this way, it is possible to track biological pathways and discover many typical tumoral features. In this context, nanoparticle-based contrast agents (CAs) can improve probe biocompatibility and biodistribution, prolonging blood half-life to achieve specific target accumulation and non-toxicity. In addition, CAs can be simultaneously delivered with drugs or, in general, therapeutic agents gathering a dual diagnostic and therapeutic effect in order to perform cancer diagnosis and treatment simultaneous. The way for personalized medicine is not so far. Herein, we report principles, characteristics, applications, and concerns of nanoparticle (NP)-based PET/MRI CAs
Turn-on fluorescence detection of protein by molecularly imprinted hydrogels based on supramolecular assembly of peptide multi-functional blocks
Supramolecular in-cavity target–peptide complex for self-reporting imprinted polymers
Optical signature of erythrocytes by light scattering in microfluidic flows
A camera-based light scattering approach coupled with a viscoelasticity-induced cell migration technique has been used to characterize the morphological properties of erythrocytes in microfluidic flows. We have obtained the light scattering profiles (LSPs) of individual living cells in microfluidic flows over a wide angular range and matched them with scattering simulations to characterize their morphological properties. The viscoelasticity-induced 3D cell alignment in microfluidic flows has been investigated by bright-field and holographic microscopy tracking, where the latter technique has been used to obtain precise cell alignment profiles in-flow. Such information allows variable cell probability control in microfluidic flows at very low viscoelastic polymer concentrations, obtaining cell measurements that are almost physiological. Our results confirm the possibility of precise, label-free analysis of individual living erythrocytes in microfluidic flows
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