Effect of SAHA and AN-7 on the viability of MF/SS cell lines, SPBL and NPBL.

<p>Viability curves based on the MTT assay of MyLa cells, Hut78 cells, (a,b), and SPBL (n = 3) (c,d) compared to NPBL (n = 8) following treatment with SAHA (a,c) and AN-7 (b,d) for 72 h. Also shown are the IC₅₀ and SI values of SAHA and AN-7 in MF/SS cell lines and SPBL and NPBL based on viability curves a-d, and their p values (e).</p

Apoptosis induction of AN-7 and SAHA in SPBL.

<p>PBL from 2 SS patients were plated at a concentration of 0.5x10⁶ cells/mL, and were then treated with SAHA 4 μM or AN-7 200 μM for 48 h. The cells were then stained with annexin V and PI. FACS plots are shown with percent of cells in each quadruplet, and the percent of cells in apoptotic cells (early + late apoptosis) are shown also in column.</p

Toxic and apoptotic effect of SAHA and AN-7 on MF/SS cell lines as a function of exposure time.

<p>Viability curves based on trypan blue staining of MyLa and Hut78 cells following short or long exposure to SAHA (a, c) or AN-7 (b, d). IC₅₀ values of short and long exposure to SAHA and AN-7 in MF/SS cell lines based on viability curves a-d (e). Apoptosis curves based on FACS analysis of annexin V and PI staining (f-i). Percent of apoptotic MyLa cells (early + late apoptosis) after short or long exposure to SAHA (f) or AN-7 (g), and apoptotic Hut78 cells after short or continuous exposure to SAHA (h) or AN-7 (i).</p

Effect of SAHA and AN-7 on specific protein expression and modification in MF/SS cell lines.

<p>Immunoblot of apoptotic and proapoptotic proteins in MyLa and Hut78 cells treated with SAHA 10 μM or AN-7 300 μM for the indicated periods (a). Basal HDAC1 protein expression in NPBL and MF/SS cell lines (b) and in MF/SS cell lines treated with SAHA 10 μM or AN-7 300 μM (c). Acetylated H3 in the nuclear lysate of MF/SS cell lines treated with and the same concentrations of SAHA and AN-7 for the indicated periods (d).</p