Protein folding and unfolding: proline cis-trans isomerization at the c subunits of F1FO-ATPase might open a high conductance ion channel

The c subunits, which constitute the c-ring apparatus of the F1 FO -ATPase, could be the main components of the mitochondrial permeability transition pore (mPTP). The well-known modulator of the mPTP formation and opening is the cyclophilin D (CyPD), a peptidyl-prolyl cis-trans isomerase. On the loop, which connects the two hairpin α-helix of c subunit, is present the unique proline residue (Pro40 ) that could be a biological target of CyPD. Indeed, the proline cis-trans isomerization might provide the switch that interconverts the open/closed states of the pore by pulling out the c-ring lipid plug

Optical variability of the strong-lined and X-ray bright source 1WGA J0447.9-0322

We present the historic light curve of 1WGA J0447.9-0322, spanning the time interval from 1962 to 1991, built using the Asiago archive plates. The source shows small fluctuations of about 0.3 mag around B=16 until 1986 and a fast dimming of its average level by about 0.5 mag after that date, again with small short term variations. The variability pattern is within the values shown by other QSOs with long term monitoring, notwithstanding its high X-ray/optical ratio. We present also its overall SED using literature data and recent UV-optical SWIFT observations.Comment: 17 pages, 4 figures, accepted by The Astronomical Journal. Table 2 available upon reques

Inflammation, Mitochondria and Natural Compounds Together in the Circle of Trust

Human diseases are characterized by the perpetuation of an inflammatory condition in which the levels of Reactive Oxygen Species (ROS) are quite high. Excessive ROS production leads to DNA damage, protein carbonylation and lipid peroxidation, conditions that lead to a worsening of inflammatory disorders. In particular, compromised mitochondria sustain a stressful condition in the cell, such that mitochondrial dysfunctions become pathogenic, causing human disorders related to inflammatory reactions. Indeed, the triggered inflammation loses its beneficial properties and turns harmful if dysregulation and dysfunctions are not addressed. Thus, reducing oxidative stress with ROS scavenger compounds has proven to be a successful approach to reducing inflammation. Among these, natural compounds, in particular, polyphenols, alkaloids and coenzyme Q10, thanks to their antioxidant properties, are capable of inhibiting the activation of NF-κB and the expression of target genes, including those involved in inflammation. Even more, clinical trials, and in vivo and in vitro studies have demonstrated the antioxidant and anti-inflammatory effects of phytosomes, which are capable of increasing the bioavailability and effectiveness of natural compounds, and have long been considered an effective non-pharmacological therapy. Therefore, in this review, we wanted to highlight the relationship between inflammation, altered mitochondrial oxidative activity in pathological conditions, and the beneficial effects of phytosomes. To this end, a PubMed literature search was conducted with a focus on various in vitro and in vivo studies and clinical trials from 2014 to 2022

The Long Term Optical Variability of the BL Lac object S5 0716+714: Evidence for a Precessing Jet

We present the historic light curve of the BL Lac object S5 0716+714, spanning the time interval from 1953 to 2003, built using Asiago archive plates and our recent CCD observations, together with literature data. The source shows an evident long term variability, over which well known short term variations are superposed. In particular, in the period from 1961 to 1983 the mean brightness of S5 0716+714 remained significantly fainter than that observed after 1994. Assuming a constant variation rate of the mean magnitude we can estimate a value of about 0.11 magnitude/year. The simultaneous occurrence of decreasing ejection velocities of superluminal moving components in the jet reported by Bach et al. (2005) suggests that both phenomena are related to the change of the direction of the jet to the line of sight from about 5 to 0.7 degrees for an approximately constant bulk Lorentz factor of about 12. A simple explanation is that of a precessing relativistic jet, which should presently be close to the smallest orientation angle. One can therefore expect in the next ten years a decrease of the mean brightness of about 1 magnitude.Comment: to appear on The Astronomical Journal, 17 pages, 7 figures. Fig.2 is given as a separated jpg fil

Understanding differential aspects of microdiffusion (channeling) in the Coenzyme Q and Cytochrome c regions of the mitochondrial respiratory system

Over the past decades, models of the organization of mitochondrial respiratory system have been controversial. The goal of this perspective is to assess this “conflict of models” by focusing on specific kinetic evidence in the two distinct segments of Coenzyme Q- and Cytochrome c-mediated electron transfer. Respiratory supercomplexes provide kinetic advantage by allowing a restricted diffusion of Coenzyme Q and Cytochrome c, and short-range interaction with their partner enzymes. In particular, electron transfer from NADH is compartmentalized by channeling of Coenzyme Q within supercomplexes, whereas succinate oxidation proceeds separately using the free Coenzyme Q pool. Previous evidence favoring Coenzyme Q random diffusion in the NADH-dependent electron transfer is due to downstream flux interference and misinterpretation of results. Indeed, electron transfer by complexes III and IV via Cytochrome c is less strictly dependent on substrate channeling in mammalian mitochondria. We briefly describe these differences and their physiological implications

The BeppoSAX view of the hot cluster Abell 2319

We present results from a BeppoSAX observation of the rich cluster Abell 2319. The broad band spectrum (2-50 keV) of the cluster can be adequately represented by an optically thin thermal emission model with a temperature of 9.6+/-0.3 keV and a metal abundance of 0.25+/-0.03 in solar units, and with no evidence of a hard X-ray excess in the PDS spectrum. From the upper limit to the hard tail component we derive a lower limit of ~0.04 \muG for the volume-averaged intracluster magnetic field. By performing spatially resolved spectroscopy in the medium energy band (2-10 keV), we find that the projected radial temperature and metal abundance profiles are constant out to a radius of 16 arcmin (1.4 Mpc). A reduction of the temperature of 1/3, when going from the cluster core out to 16 arcmin, can be excluded in the present data at the 99% confidence level. From the analysis of the temperature and abundance maps we find evidence of a temperature enhancement and of an abundance decrement in a region localized 6 arcmin--8 arcmin NE of the core, where a merger event may be taking place. Finally, the temperature map indicates that the subcluster located NW of the main cluster may be somewhat cooler than the rest of the cluster.Comment: To appear in ApJ-Letter

Optical and Radio monitoring of S5 1803+74

The optical (BVRI) and radio (8.4 GHz) light curves of S5 1803+784 on a time span of nearly 6 years are presented and discussed. The optical light curve showed an overall variation greater than 3 mag, and the largest changes occured in three strong flares. No periodicity was found in the light curve on time scales up to a year. The variability in the radio band is very different, and shows moderate oscillations around an average constant flux density rather than relevant flares, with a maximum amplitude of $\sim$30%, without a simultaneous correspondence between optical and radio luminosity. The optical spectral energy distribution was always well fitted by a power law. The spectral index shows small variations and there is indication of a positive correlation with the source luminosity. Possible explanations of the source behaviour are discussed in the framework of current models.Comment: 25 pages, 12 figure

S5 1803+78 Revisited

We report on our optical monitoring of the BL Lac object S5 1803+78 from 1996 to 2011. The source showed no clear periodicity, but a time scale of about 1 300 days between major flares is possibly present. No systematic trend of the color index with flux variations is evident, at variance with other BL Lacs. In one flare, however, the source was bluer in the rising phase and redder in the falling one. Two ?-ray flares were detected by Fermi-GST during our monitoring: on the occasion of only one of them we found simultaneous optical brightening. A one-zone Synchrotron Self Compton (SSC) model appears too simple to explain the source behavior