ANTICANCER AND ANTIOXIDANT ACTIVITY OF METHANOL EXTRACT OF HIPPOPHAE SALICIFOLIA IN EAC INDUCED SWISS ALBINO MICE

Objective: Anticancer activity of methanol extract of Hippophae salicifolia (MEHS) bark was evaluated using the Ehrlich Ascites Carcinoma (EAC) cells on Swiss albino mice.Methods: In vitro cytotoxicity assay has been evaluated by using the trypan blue and MTT assay method. The determination of in vivo anticancer activity was performed using EAC cells (2 × 106) induced mice groups (n = 12). After treatment with MEHS at the doses of 50 and 100 mg/kg b. w. respectively for 9 days, half of the mice of each group were sacrificed and the rest were kept for increase in life span determination. The anticancer potential of MEHS was assessed by evaluating tumor volume, viable and nonviable tumor cell count, tumor weight, hematological parameters and biochemical estimations. Furthermore, tissue antioxidant parameters were checked from liver homogenate.Results: In MEHS treated groups (50 and 100 mg/kg b. w.) tumor volume, tumor weight, viable cell count was significantly decreased as compared to that of the EAC control group. Life span increased by 34% and 43% in a dose dependant manner as compared to EAC control group. The hematological, biochemical and liver tissue antioxidant parameter are significantly (p < 0.05) restored towards the normal level after treatment with MEHS.Conclusion: From the above study it can be concluded that the MEHS has significant anticancer activity in the dose dependent manner

Anti - diabetic activity of aeschynanthus sikkimensis (clarke) stapf. extract in streptozotocin induced hyperglycemic rats

Objective: The research was design to conduct anti-diabetic activity of methanol extract of Aeschynanthus sikkimensis (Clarke) Stapf. in streptozotocin induce hyperglycemic rats. Material and method: Streptozotocin 35mg/kg body weight was injected intraperitoneal to produce hyperglycemia rats which were pretreated with a high fat diet.72 hours after introduction of STZ, the hyperglycemic rats received MEAS at dose 200 and 400 mg/kg body weight for 28 days. Reference drug used was Glibenclamide (0.5 mg/kg, orally). During the 28 days treatment fasting blood glucose level were determined on every 7th day. Serum and hepato renal biochemical parameters were estimated. Result: MEAS at the dose of 200 and 400mg/kg outstandingly (P <0.05) and dosage dependently reduce blood glucose levels towards normal as differentiate to STZ control group Serum and hepato renal biochemical parameter were altogether (P<0.05) restore towards the typical levels in MEAS sedated rats as differentiated to STZ control group. Conclusion: It may be concluded that the whole plant extract of Aeschynanthus sikkimensis (Clarke) Stapf which belong to the family Gesneriaceae showed encouraging hypoglycemic action in STZ induced diabetic rats

Anti - Diabetic Activity of Aeschynanthus Sikkimensis (Clarke) Stapf. Extract in Streptozotocin Induced Hyperglycemic Rats

Objective: The research was design to conduct anti-diabetic activity of methanol extract of Aeschynanthus sikkimensis (Clarke) Stapf. in streptozotocin induce hyperglycemic rats. Material and method: Streptozotocin 35mg/kg body weight was injected intraperitoneal to produce hyperglycemia rats which were pretreated with a high fat diet.72 hours after introduction of STZ, the hyperglycemic rats received MEAS at dose 200 and 400 mg/kg body weight for 28 days. Reference drug used was Glibenclamide (0.5 mg/kg, orally). During the 28 days treatment Fasting blood glucose level were determined on every 7th day. Serum and hepato renal biochemical parameters were estimated. Result: MEAS at the dose of 200 and 400mg/kg outstandingly (P <0.05) and dosage dependently reduce blood glucose levels towards normal as differentiate to STZ control group Serum and hepato renal biochemical parameter were altogether (P<0.05) restore towards the typical levels in MEAS sedated rats as differentiated to STZ control group. Conclusion: It may be concluded that the whole plant extract of Aeschynanthus sikkimensis (Clarke) Stapf which belong to the family Gesneriaceae showed encouraging hypoglycemic action in STZ induced diabetic rats