12 research outputs found
A Nocardia cyriacigeorgica strain causing severe bovine mastitis
Nocardia cyriacigeorgica ΡΠ΅ ΡΠ·ΡΠΎΡΠ½ΠΈΠΊ ΠΊΠΎΡΠΈ ΡΠ΅ ΡΠ²Π΅ ΡΠ΅ΡΡΠ΅ ΠΈΠ·ΠΎΠ»ΡΡΠ΅ Ρ ΡΠ»ΡΡΠ°ΡΠ΅Π²ΠΈΠΌΠ° Π½ΠΎΠΊΠ°ΡΠ΄ΠΈΠΎΠ·Π΅ ΡΡΠ΄ΠΈ, ΠΌΠ΅ΡΡΡΠΈΠΌ ΡΠ΅Π³ΠΎΠ²Π° ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΠΊΠ°ΡΠΈΡΠ° Π΄ΠΎ Π½ΠΈΠ²ΠΎΠ° Π²ΡΡΡΠ΅ ΠΏΡΠ΅Π΄ΡΡΠ°Π²ΡΠ° ΠΈΠ·Π°Π·ΠΎΠ² Π·Π° Π²Π΅ΡΠΈΠ½Ρ ΠΌΠΈΠΊΡΠΎΠ±ΠΈΠΎΠ»ΠΎΡΠΊΠΈΡ
Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠΈΡΠ°. ΠΠ½ΡΠ΅ΠΊΡΠΈΡΠ΅ ΠΈΠ·Π°Π·Π²Π°Π½Π΅ ΠΎΠ²ΠΎΠΌ Π°ΠΊΡΠΈΠ½ΠΎΠΌΠΈΡΠ΅ΡΠΎΠΌ ΡΠ΅ ΡΠ΅Π»Π°ΡΠΈΠ²Π½ΠΎ ΡΠ΅ΡΠΊΠΎ ΡΡΡΡΠ΅ΡΡ Ρ Π²Π΅ΡΠ΅ΡΠΈΠ½Π°ΡΡΠΊΠΎΡ ΠΊΠ»ΠΈΠ½ΠΈΡΠΊΠΎΡ ΠΏΡΠ°ΠΊΡΠΈ ΠΏΡΠΈ ΡΠ΅ΠΌΡ Π΄ΠΎΡΡΡΠΏΠ½ΠΈ Π»ΠΈΡΠ΅ΡΠ°ΡΡΡΠ½ΠΈ ΠΏΠΎΠ΄Π°ΡΠΈ ΠΎΠ±ΡΡ
Π²Π°ΡΠ°ΡΡ ΠΌΠ°Π»ΠΈ Π±ΡΠΎΡ ΠΎΠΏΠΈΡΠ°Π½ΠΈΡ
ΡΠ»ΡΡΠ°ΡΠ΅Π²Π° ΠΏΠΈΠΎΠ³ΡΠ°Π½ΡΠ»ΠΎΠΌΠ°ΡΠΎΠ·Π½ΠΈΡ
Π»Π΅Π·ΠΈΡΠ° ΠΊΠΎΠ΄ ΠΆΠΈΠ²ΠΎΡΠΈΡΠ°. ΠΡΠΈΠΊΠ°Π·Π°Π½Π° ΡΡΡΠ΄ΠΈΡΠ° ΠΎΠΏΠΈΡΡΡΠ΅ ΡΠ»ΡΡΠ°Ρ ΡΠ΅ΡΠΊΠΎΠ³ ΠΌΠ°ΡΡΠΈΡΠΈΡΠ° Π³ΠΎΠ²Π΅Π΄Π° Π½Π° ΠΌΠ°Π»ΠΎΠΌ Π³Π°Π·Π΄ΠΈΠ½ΡΡΠ²Ρ Ρ ΠΠΎΡΠ½ΠΈ ΠΈ Π₯Π΅ΡΡΠ΅Π³ΠΎΠ²ΠΈΠ½ΠΈ ΠΈΠ·Π°Π·Π²Π°Π½ΠΎΠ³ ΡΠ·ΡΠΎΡΠ½ΠΈΠΊΠΎΠΌ N. cyriacigeorgica ΠΊΠΎΡΠΈ ΡΠ΅ Π±Π΅Π·ΡΡΠΏΠ΅ΡΠ½ΠΎ ΡΡΠ΅ΡΠΈΡΠ°Π½ ΠΏΡΠΈΠΌΠ΅Π½ΠΎΠΌ ΡΡΠ°Π½Π΄Π°ΡΠ΄Π½Π΅ Π°Π½ΡΠΈΠ±ΠΈΠΎΡΡΠΊΠ΅ ΡΠ΅ΡΠ°ΠΏΠΈΡΠ΅. Π‘ ΠΎΠ±Π·ΠΈΡΠΎΠΌ Π΄Π° ΠΈΠ·ΠΎΠ»Π°Ρ Π½ΠΈΡΠ΅ ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΠΊΠΎΠ²Π°Π½ ΠΏΡΠΈΠΌΠ΅Π½ΠΎΠΌ ΠΊΠΎΠ½Π²Π΅Π½ΡΠΈΠΎΠ½Π°Π»Π½ΠΈΡ
ΠΌΠΈΠΊΡΠΎΠ±ΠΈΠΎΠ»ΠΎΡΠΊΠΈΡ
ΡΠ΅Ρ
Π½ΠΈΠΊΠ°, ΠΈΠ·Π²ΡΡΠ΅Π½Π° ΡΠ΅ MALDI-ToF MS Π°Π½Π°Π»ΠΈΠ·Π° ΡΠΈΡΠΈ ΡΡ ΡΠ΅Π·ΡΠ»ΡΠ°ΡΠΈ Π±ΠΈΠ»ΠΈ Π½Π΅Π΄ΠΎΠ²ΠΎΡΠ½ΠΎ ΠΏΠΎΡΠ·Π΄Π°Π½ΠΈ. Π£ ΡΠΈΡΡ ΡΡΠ²ΡΡΠΈΠ²Π°ΡΠ° Π²ΡΡΡΠ΅ Π°Π½Π°Π»ΠΈΠ·ΠΈΡΠ°Π½Π° ΡΠ΅ ΡΠ΅ΠΊΠ²Π΅Π½ΡΠ° Π³Π΅Π½Π° 16S ΡΡΠ±ΡΠ΅Π΄ΠΈΠ½ΠΈΡΠ΅ ΡΠΈΠ±ΠΎΠ·ΠΎΠΌΠ°Π»Π½Π΅ ΡΠΈΠ±ΠΎΠ½ΡΠΊΠ»Π΅ΠΈΠ½ΡΠΊΠ΅ ΠΊΠΈΡΠ΅Π»ΠΈΠ½Π΅ (16S rRNK). ΠΠ°Π²Π΅Π΄Π΅Π½ΠΈ Π½Π°Π»Π°Π· ΡΠ΅ Π΄ΠΎΠ΄Π°ΡΠ½ΠΎ ΠΏΠΎΡΠ²ΡΡΠ΅Π½ ΠΏΡΠΈΠΌΠ΅Π½ΠΎΠΌ ΡΠ΅Ρ
Π½ΠΈΠΊΠ΅ ΡΠ΅ΠΊΠ²Π΅Π½ΡΠΈΡΠ°ΡΠ° Π²ΠΈΡΠ΅ Π³Π΅Π½ΡΠΊΠΈΡ
Π»ΠΎΠΊΡΡΠ° (MLST) ΠΏΡΠΈ ΡΠ΅ΠΌΡ ΡΡ ΠΊΠΎΡΠΈΡΡΠ΅Π½Π΅ ΡΠ΅ΠΊΠ²Π΅Π½ΡΠ΅ gyrB, 16S rRNK, secA1 ΠΈ hsp65. ΠΠ΅ΡΠΎΠ΄Π° Π΄ΠΈΡΠΊ Π΄ΠΈΡΡΠ·ΠΈΡΠ΅ Π·Π° ΠΈΡΠΏΠΈΡΠΈΠ²Π°ΡΠ΅ ΠΎΡΠ΅ΡΡΠΈΠ²ΠΎΡΡΠΈ ΠΈΠ·ΠΎΠ»Π°ΡΠ° N. cyriacigeorgica Π½Π° Π°Π½ΡΠΈΠ±ΠΈΠΎΡΠΈΠΊΠ΅ ΡΠ΅ ΠΏΡΠΈΠΌΠ΅ΡΠ΅Π½Π° Ρ ΡΠΊΠ»Π°Π΄Ρ ΡΠ° ΠΏΡΠ΅ΠΏΠΎΡΡΡΠ΅Π½ΠΈΠΌ ΠΏΡΠ±Π»ΠΈΠΊΠΎΠ²Π°Π½ΠΈΠΌ ΡΠΏΡΡΡΡΠ²ΠΈΠΌΠ°. ΠΠΎΠ»ΠΈΠΊΠΎ ΡΠ΅ Π°ΡΡΠΎΡΠΈΠΌΠ° ΠΏΠΎΠ·Π½Π°ΡΠΎ, ΠΎΠ²ΠΎ ΠΏΡΠ΅Π΄ΡΡΠ°Π²ΡΠ° ΠΏΡΠ²ΠΈ ΡΠ»ΡΡΠ°Ρ ΠΌΠ°ΡΡΠΈΡΠΈΡΠ° Π³ΠΎΠ²Π΅Π΄Π° ΠΈΠ·Π°Π·Π²Π°Π½ ΡΠ·ΡΠΎΡΠ½ΠΈΠΊΠΎΠΌ N. cyriacigeorgica Ρ ΠΠ²ΡΠΎΠΏΠΈ, Π°Π»ΠΈ ΠΈ ΠΏΡΠ²ΠΈ ΠΈΠ·ΠΎΠ»Π°Ρ Nocardia spp. ΠΏΠΎΡΠ΅ΠΊΠ»ΠΎΠΌ ΠΎΠ΄ ΠΆΠΈΠ²ΠΎΡΠΈΡΠ° Π·Π° ΡΠΈΡΡ ΡΠ΅ ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΠΊΠ°ΡΠΈΡΡ ΠΏΡΠΈΠΌΠ΅ΡΠ΅Π½ MLST.Although Nocardia cyriacigeorgica is recognized as a cause of human nocardiosis and is regarded as an emerging pathogen, many clinical laboratories find it difficult to identify this organism at the species level. The infections caused by this actinomycete are relatively uncommon in veterinary clinical practice, and the limited information currently available mostly relates to several cases of pyogranulomatous lesions in animals. We describe a case of severe bovine mastitis in a small holding in Bosnia and Herzegovina caused by N. cyriacigeorgica that did not improve after standard antibiotic therapy. The causative agent could not be identified using standard microbiological techniques and MALDI-ToF MS analysis was performed, yielding unclear results. Subsequently, the species N. cyriacigeorgica was confirmed by 16S rRNA sequence analysis, which was further validated by multilocus sequence analysis (MLSA) using the gyrB, 16S rRNA, secA1, and hsp65 sequences. The disc diffusion method was used to test for antibiotic susceptibility in accordance with published guidelines. To the best of our knowledge, this is the first report of N. cyriacigeorgica isolation from a clinical case of bovine mastitis in Europe, as well as the first time a strain of Nocardia spp. isolated from animals has been distinguished using the MLSA method.Zbornik kratkih sadrΕΎaj
Differences in antimicrobial consumption, prescribing and isolation rate of multidrug resistant Klebsiella pneumoniae, Pseudomonas aeruginosa and Acinetobacter baumannii on surgical and medical wards.
In order to provide guidance data for clinically rational use of an antibiotics consuption, prescribing and prevalence of multidrug resistant (MDR) Klebsiella pneumoniae, Pseudomonas aeruginosa and Acinetobacter baumannii were monitored on the surgical (S) and medical (M) wards of the University Hospital Center "Dr. Dragisa Misovic-Dedinje" (Belgrade, Serbia), in the study period from 2012 to 2015. Appropriateness of antimicrobial use was evaluated using the Global-Prevalence Survey method designed by the University of Antwerp. The percentages of MDR pathogens relative to the total number of isolates of K. pneumoniae and P. aeruginosa were higher on the S (86.2% and 49.1%) than on the M (63.2% and 36.9%) wards. The percentage of MDR A. baumannii was not different between S (93.7%) and M (79.5%) wards. An overall antibiotics consumption (defined daily doses/100 bed-days) during study was 369.7 and 261.5 on the S and M wards, respectively. A total of 225 prescriptions of antimicrobials were evaluated in138 adults admitted to wards on the day of the survey. The percentage of antimicrobials prescribed for prophylaxis on the M and S wards were 0% and 25%, respectively. Therapies were more frequently empiric (S, 86.8% and M, 80%). The percentages of medical errors on the S and M wards were 74.6% and 27.3%, respectively. The quality indicators for antibiotic prescribing on the S and M wards were as follows: the incorrect choice of antimicrobials (35.6% vs. 20.0%), inappropriate dose interval (70.6% vs. 16.9%) or duration of therapy (72.5% vs. 23.1%), a non-documented stop/review data (73.6% vs. 16.9%) and divergence from guidelines (71.9% vs. 23.1%). Treatment based on biomarkers was more common on the M wards as compared to the S wards. The increasing prevalence of MDR pathogens, a very high consumption and incorrect prescribing of antimicrobials need special attention, particularly on the S wards
Quality of antimicrobial prescribing obtained by Global-Point Prevalence Survey method.
<p>Quality of antimicrobial prescribing obtained by Global-Point Prevalence Survey method.</p
Reasons for antibiotics prescribing obtained by Global-Point Prevalence Survey method.
<p>Reasons for antibiotics prescribing obtained by Global-Point Prevalence Survey method.</p
Association between antimicrobial consumption and isolation rate of multi drugs resistant <i>K</i>. <i>pneumoniae</i>, <i>P</i>. <i>aeruginosa</i> and <i>A</i>. <i>baumannii</i> at surgical (A) and medical (B) wards.
<p>Association between antimicrobial consumption and isolation rate of multi drugs resistant <i>K</i>. <i>pneumoniae</i>, <i>P</i>. <i>aeruginosa</i> and <i>A</i>. <i>baumannii</i> at surgical (A) and medical (B) wards.</p
Resistance rate (%) of <i>Klebsiella pneumoniae</i>, <i>Pseudomonas aeruginosa</i> and <i>Acinetobacter baumannii</i> strains isolated from 2012β2015.
<p>Resistance rate (%) of <i>Klebsiella pneumoniae</i>, <i>Pseudomonas aeruginosa</i> and <i>Acinetobacter baumannii</i> strains isolated from 2012β2015.</p
Annual distribution of isolation rates and patients on surgical and medical wards.
<p>Annual distribution of isolation rates and patients on surgical and medical wards.</p
Types and frequency of appearance of bacterial strains from 2012 to 2015.
<p>Types and frequency of appearance of bacterial strains from 2012 to 2015.</p
Main indications for antimicrobial therapy from 2012 to 2015.
<p>Main indications for antimicrobial therapy from 2012 to 2015.</p
Trends of antibiotic consumption by individual drugs on surgical (A) and medical (B) wards from 2012 to 2015.
<p>A. *There was a statistically significant decrease in the use of amoxicillin/clavulanate (b = -2.881; p = 0.014). B. *There was a statistically significant decrease in the use of ceftriaxone (b = -2.925; p = 0.047). **There was a statistically significant increase in the use of levofloxacin (b = 2.745; p = 0.014). ***There was a statistically significant increase in the use of metronidazole (b = 1.385; p = 0.029).</p