Two-dimensional gel electrophoresis patterns of proteins extracted from sugarcane leaves of the RB867515 cultivar watered (A) with distilled water and (B) after being subjected to 100 mM NaCl for 48 days.

<p>The strips used were 13-linear pH gradient of 3-11, stained with Coomassie G-250. The proteins indicated by numbers (1-4) correspond to those showing at least 1.5-fold difference in expression levels between the two different treatments; proteins indicated with letters (a-h) represent proteins with no difference in expression profile.</p

Net CO₂-exchange (µmol.m⁻²s⁻¹) for (A) cultivar RB855536 and (B) cultivar RB867515 over time.

<p>Leaf water potential (MPa) in sugarcane leaves during 48 days of salt treatment for (C) cultivar RB855536; and (D) cultivar RB867515. Values are presented as mean ± SD (n = 6). "•" are control plants and "▪" are salt-treated plants. *Significant at p≤0.05.</p

Cultivar RB867515 sugarcane leaf proteins differentially expressed between water-treated and salt-treated plants.

<p>Proteins were trypsin digested and identified by MALDI-ToF/ToF MS. “1-4” are differentially expressed proteins and “a-h” are proteins with no difference in protein expression.</p

Shoot dry mass for cultivars(A) RB855536 and (B) RB867515; and root dry mass for cultivars (C) RB855536 and (D) RB867515 after being subjected to 48 days of salt stress (100 mM NaCl).

<p>Lipid peroxidation levels (MDA) in sugarcane leaves during 48 days of salt stress (100 mM NaCl) for (E) cultivar RB855536 and (F) RB867515. Values are presented as mean ± SD (n = 6 plants). "•" are control plants and "▪" are salt-treated plants. *Significant at p≤0.05.</p

Relative volume of protein spots corresponding to differentially expressed proteins.

<p>(A) Quantification of protein expression and (B) Image of protein spots on gels. The left panel shows protein expression on water-treated control plants and right panel shows protein expression on salt-treated plants at day 48. Proteins were identified by mass spectrometry as being (1) fructose 1,6-bisphosphatealdolase; (2) glyceraldehyde-3-phostate dehydrogenase; (3) germin-like protein (4) HSP70. Bars show the mean values of replicate spots.</p