Colecistectomia videolaparoscópica em paciente submetido a transplante cardíaco

Cardiac transplant has been performed with an increased frequency as the treatment for end-stage cardiac disease. Although cholelithiasis is more frequent in both pretransplant and posttransplant patients, no standard management approach exists. Pretransplant patients are well recognized for cardiac events, and posttransplant immunossupressed patients are at a considerable risk for septic complications. Because the first presentation of gallstones in this population is often acute cholecystitis, asymptomatic calculi cannot be considered benign and it seems reasonable to recommend pretransplant screening and posttransplant surveillance for gallstones. Prophylatic laparoscopic cholecistectomy should be undertaken in the stable patient to avoid the substantial mortality associated with postoperative acute cholecystitis and urgent cholecystectomy. In this case report we present a 44 year-old male with acute cholecystitis after cardiac transplantation who was submitted to a safe laparoscopic cholecystectomy one year and seven months later

Bloqueio por clipagem de gânglios simpáticos torácicos no tratamento da hiper-hidrose

FUNDAMENTOS: Bloqueio simpático videotoracoscópico no tratamento da hiper-hidrose é realizado por clipagem do tronco simpático, com possibilidade de reversão em casos de sudorese compensatória intensa. OBJETIVO: Avaliar sucesso terapêutico, satisfação e sudorese compensatória nos pacientes submetidos a essa técnica. MÉTODO: Estudo prospectivo em que 45 pacientes foram divididos em dois grupos. Grupo I: um paciente com hiper-hidrose palmar e 20 com hiper-hidrose palmar e plantar submetidos a bloqueio de T3; Grupo II: quatro pacientes com hiper-hidrose axilar , dois com hiper-hidrose axilar e palmar, dois com hiper-hidrose axilar e plantar e 16 com hiperidrose axilar, palmar e plantar submetidos a bloqueio de T3 e T4. RESULTADOS: No grupo I 95,2% dos pacientes tinham hiper-hidrose palmar e plantar, e no grupo II 66,7% tinham hiperidrose axilar, palmar e plantar. Na região palmar, resultados excelentes ou bons ocorreram em 95,3% do grupo I e em 94,4% do grupo II; na região plantar 40% do grupo I e 44,5% do grupo II apresentaram bons resultados; e na região axilar, 95,8% relataram resultados excelentes ou bons. Em seis meses, havia sudorese compensatória em 76,2% do grupo I e 91,7% do grupo II, mas a sudorese compensatória intensa ocorreu em apenas três pacientes do grupo II. CONCLUSÕES: Esse tratamento foi eficiente para o tratamento da hiper-hidrose. Ao final de seis meses, todos os pacientes do grupo I e 95,9% dos pacientes do grupo II estavam satisfeitos com os resultados

Regeneration of Tracheal Tissue in Partial Defects Using Porcine Small Intestinal Submucosa

Background. Surgical correction of tracheal defects is a complex procedure when the gold standard treatment with primary end-to-end anastomosis is not possible. An alternative treatment may be the use of porcine small intestinal submucosa (SIS). It has been used as graft material for bioengineering applications and to promote tissue regeneration. The aim of this study was to evaluate whether SIS grafts improved tracheal tissue regeneration in a rabbit model of experimental tracheostomy. Methods. Sixteen rabbits were randomized into two groups. Animals in the control group underwent only surgical tracheostomy, while animals in the SIS group underwent surgical tracheostomy with an SIS graft covering the defect. We examined tissues at the site of tracheostomy 60 days after surgery using histological analysis with hematoxylin and eosin (H&E) staining and analyzed the perimeter and area of the defect with Image-Pro® PLUS 4.5 (Media Cybernetics). Results. The average perimeter and area of the defects were smaller by 15.3% (p=0.034) and 21.8% (p=0.151), respectively, in the SIS group than in the control group. Histological analysis revealed immature cartilage, pseudostratified ciliated epithelium, and connective tissue in 54.5% (p=0.018) of the SIS group, while no cartilaginous regeneration was observed in the control group. Conclusions. Although tracheal SIS engraftment could not prevent stenosis in a rabbit model of tracheal injury, it produced some remarkable changes, efficiently facilitating neovascularization, reepithelialization, and neoformation of immature cartilage

Fluorescence properties of curcumin-loaded nanoparticles for cell tracking

Background: Posttransplant cell tracking, via stem cell labeling, is a crucial strategy for monitoring and maximizing benefits of cell-based therapies. The structures and functionalities of polysaccharides, proteins, and lipids allow their utilization in nanotechnology systems. Materials and methods: In the present study, we analyzed the potential benefit of curcumin-loaded nanoparticles (NPC) using Vero cells (in vitro) and NPC-labeled adipose-derived mesenchymal stem cells (NPC-ADMSCs) (in vivo) in myocardial infarction and sciatic nerve crush preclinical models. Thereafter, transplantation, histological examination, real time imaging, and assessment of tissue regeneration were done. Results: Transplanted NPC-ADMSCs were clearly identified and revealed potential benefit when used in cell tracking. Conclusion: This approach may have broad applications in modeling labeled transplanted cells and in developing improved stem cell therapeutic strategies.Fil: Mogharbel, Bassam Felipe. Pelé Pequeno Príncipe Institute; BrasilFil: Francisco, Julio Cesar. Pelé Pequeno Príncipe Institute; BrasilFil: Irioda, Ana Carolina. Pelé Pequeno Príncipe Institute; BrasilFil: Dziedzic, Dilcele Silva Moreira. Pelé Pequeno Príncipe Institute; BrasilFil: Ferreira, Priscila Elias. Pelé Pequeno Príncipe Institute; BrasilFil: de Souza, Daiany. Pelé Pequeno Príncipe Institute; BrasilFil: de Souza, Carolina Maria Costa Oliveira. Pelé Pequeno Príncipe Institute; BrasilFil: Neto, Nelson Bergonse. Pelé Pequeno Príncipe Institute; BrasilFil: Guarita Souza, Luiz Cesar. Universidade Federal do Paraná; BrasilFil: Franco, Celia Regina Cavichiolo. Universidade Federal do Paraná; BrasilFil: Nakamura, Celso Vataru. Universidade Estadual de Maringá; BrasilFil: Kaplum, Vanessa. Universidade Estadual de Maringá; BrasilFil: Mazzarino, Letícia. Universidade Federal de Santa Catarina; BrasilFil: Lemos Senna, Elenara. Universidade Federal de Santa Catarina; BrasilFil: Borsali, Redouan. Universite Grenoble Alpes; FranciaFil: Soto, Paula Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Setton, Clara Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Abdelwahid, Eltyeb. Northwestern University; Estados UnidosFil: Athayde Teixeira de Carvalho, Katherine. Pelé Pequeno Príncipe Institute; Brasi