IgG and IgA antibody response against selected peptides in shigellosis patients’ versus control sera.

<p>IgG and IgA antibody response against selected peptides in shigellosis patients’ versus control sera.</p

IgA antibody response in BALB/c mice against all five peptides and their mixture inoculated by intranasal route.

<p>Antibody titers in sera of mice primed intranasally with peptide + CFA and boosted twice with peptide + IFA are shown. The X-axis depicts all five peptides, their mixture inoculated with adjuvant and respective control (PBS+ adjuvant) for each peptide. OD values of peptides vs. controls are depicted on Y- axis. Absorbance was measured at 450nm. Data collected from 6 mice per group (2 separate experiments) are expressed as geometric mean concentration (± 2SD) at serum dilution of 1:160. Statistical significance was determined by Mann-Whitney U test and the p-value of < 0.05 was taken as significant.</p

Different serotypes and strains of <i>Shigella</i> used in local database creation.

<p>Different serotypes and strains of <i>Shigella</i> used in local database creation.</p

TNF-α levels in BALB/c mice against all peptides and their mixture inoculated by intraperitoneal route.

<p>On the X-axis are shown all five peptides with the adjuvant, their mixture and controls (PBS+ Adjuvant) inoculated intraperitoneally. Mice were primed with peptide + CFA and two booster doses at two weeks interval each were given with peptide + IFA. TNF-α levels in pg/ml in the undiluted sera are shown on Y-axis. Data collected from 6 mice per group (2 separate experiments) are expressed as geometric mean concentration (± 2SD). Statistical significance was determined by Mann-Whitney U test and the p-value of < 0.05 was taken as significant.</p

IFN-γ levels in BALB/c mice against all peptides and their mixture inoculated by intranasal route.

<p>On the X-axis are shown all five peptides with the adjuvant, their mixture and controls (PBS + Adjuvant) for each peptide inoculated intranasally. Mice were primed with peptide + CFA and two booster doses at two weeks interval each were given with peptide + IFA. IFN-γ levels in pg/ml in the undiluted sera are shown on Y-axis. Data collected from 6 mice per group (2 separate experiments) are expressed as geometric mean concentration (± 2SD). Statistical significance was determined by Mann-Whitney U test and the p-value of < 0.05 was taken as significant.</p

IgG antibody response in BALB/c mice against all five peptides and their mixture inoculated by intraperitoneal route.

<p>Antibody titers in sera of mice primed intraperitoneally with peptide + CFA and boosted twice with peptide + IFA are shown. The X-axis depicts all five peptides, their mixture inoculated with adjuvant and respective control (PBS+ adjuvant) for each peptide. OD values of peptides vs. controls are depicted on Y- axis. Absorbance was measured at 450nm. Data collected from 6 mice per group (2 separate experiments) are expressed as geometric mean concentration (± 2SD) at serum dilution of 1:160. Statistical significance was determined by Mann-Whitney U test and the p-value of < 0.05 was taken as significant.</p

IgG antibody response in BALB/c mice against all five peptides and their mixture inoculated by subcutaneous route.

<p>Antibody titers in sera of mice primed subcutaneously with peptide + CFA and boosted twice with peptide + IFA are shown. The X-axis depicts all five peptides, their mixture inoculated with adjuvant and respective control (PBS+ adjuvant) for each peptide. OD values of peptides vs. controls are depicted on Y- axis. Absorbance was measured at 450nm. Data collected from 6 mice per group (2 separate experiments) are expressed as geometric mean concentration (± 2SD) at serum dilution of 1:160. Statistical significance was determined by Mann-Whitney U test and the p-value of < 0.05 was taken as significant.</p

TNF-α levels in BALB/c mice against all peptides and their mixture inoculated by subcutaneous route.

<p>On the X-axis are shown all five peptides with the adjuvant, their mixture and controls (PBS + Adjuvant) inoculated subcutaneously. Mice were primed with peptide + CFA and two booster doses at two weeks interval each were given with peptide + IFA. TNF-α levels in pg/ml in the undiluted sera are shown on Y-axis. Data collected from 6 mice per group (2 separate experiments) are expressed as geometric mean concentration (± 2SD). Statistical significance was determined by Mann-Whitney U test and the p-value of < 0.05 was taken as significant.</p

The peptides identified by in-silico analysis and used for in-vivo experiments.

<p>The peptides identified by in-silico analysis and used for in-vivo experiments.</p

IgA antibody response in BALB/c mice against all five peptides and their mixture inoculated by subcutaneous route.

<p>Antibody titers in sera of mice primed subcutaneously with peptide + CFA and boosted twice with peptide + IFA are shown. The X-axis depicts all five peptides, their mixture inoculated with adjuvant and respective control (PBS+ adjuvant) for each peptide. OD values of peptides vs. controls are depicted on Y- axis. Absorbance was measured at 450nm. Data collected from 6 mice per group (2 separate experiments) are expressed as geometric mean concentration (± 2SD) at serum dilution of 1:160. Statistical significance was determined by Mann-Whitney U test and the p-value of < 0.05 was taken as significant.</p