Influence of L-lysine sulfate on containing of vitamins and minerals in the body of broiler chickens

Experimental researches have shownthatthe L-lysine sulfate (the product of microbiological synthesis) has a positive effect on metabolism of vitamins A, E, C in the liver and minerals calcium and phosphorus in the blood and bones of broiler chickens. The dose of L-lysine sulfate of 800 mg-kg" 1 and 1000 mg-kg-1 weight body has influence on increasing vitamins level in the liver. Was observed, that indexes of calcium and phosphorus are increased in the blood but it’s decreased in the bones of broiler chickens. This results are necessary for Veterinary Pharmacology Consults and official practically using in agricultural sectors of Russian Federatio

Influence of L-lysine sulfate on containing of vitamins and minerals in the body of broiler chickens

Experimental researches have shownthatthe L-lysine sulfate (the product of microbiological synthesis) has a positive effect on metabolism of vitamins A, E, C in the liver and minerals calcium and phosphorus in the blood and bones of broiler chickens. The dose of L-lysine sulfate of 800 mg-kg" 1 and 1000 mg-kg-1 weight body has influence on increasing vitamins level in the liver. Was observed, that indexes of calcium and phosphorus are increased in the blood but it’s decreased in the bones of broiler chickens. This results are necessary for Veterinary Pharmacology Consults and official practically using in agricultural sectors of Russian Federatio

Comparative Study of Cytotoxic and Membranotropic Properties of Betulinic Acid-F16 Conjugate on Breast Adenocarcinoma Cells (MCF-7) and Primary Human Fibroblasts

The present study evaluates the cytotoxicity of a previously synthesized conjugate of betulinic acid (BA) with the penetrating cation F16 on breast adenocarcinoma (MCF-7) and human fibroblast (HF) cell lines, and also shows the mechanism underlying its membranotropic action. It was confirmed that the conjugate exhibits higher cytotoxicity compared to native BA at low doses also blocking the proliferation of both cell lines and causing cell cycle arrest in the G0/G1 phase. We show that the conjugate indeed has a high potential for accumulation in mitochondria, being visualized in these organelles, which is most pronounced in cancer cells. The effect of the conjugate was observed to be accompanied by ROS hyperproduction in both cancerous and healthy cells, despite the lower base level of ROS in the latter. Along with this, using artificial liposomes, we determined that the conjugate is able to influence the phase state of lipid membranes, make them more fluid, and induce nonspecific permeabilization contributing to the overall cytotoxicity of the tested agent. We conclude that the studied BA–F16 conjugate does not have significant selective cytotoxicity, at least against the studied breast cancer cell line MCF-7