The effects of finasteride (FIN) and thioacetamide (TAA) on acetylcholinesterase (AchE) activity in various brain regions.

<p>The significance of the difference was estimated by ANOVA with Fisher’s <i>post hoc</i> test (*p<0.05 and **p<0.01 <i>vs</i>. control in the same region). For details see caption for Figs <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0134434#pone.0134434.g001" target="_blank">1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0134434#pone.0134434.g002" target="_blank">2</a>.</p

The effects of finasteride (FIN) and thioacetamide (TAA) on (a) malondialdehyde (MDA) level and (b) superoxide dismutase (SOD) activity in various brain regions.

<p>Four brain regions (dorsolateral frontal cortex, hippocampus, thalamus and caudate nucleus) were isolated 24 h after administration of the last dose of TAA and crude synaptosomal fractions were prepared for determination of parameters of oxidative stress. The significance of the difference was estimated by ANOVA with Fisher’s <i>post hoc</i> test (*p<0.05 and **p<0.01 <i>vs</i>. control in the same region). For details see caption for <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0134434#pone.0134434.g001" target="_blank">Fig 1</a>.</p

The effects of finasteride (FIN) and thioacetamide (TAA) on (a) reduced glutathione (GSH) level, (b) glutathione peroxidase (GPx), (c) glutathione reductase (GR) and (d) catalase activity in various brain regions.

<p>The significance of the difference was estimated by ANOVA with Fisher’s <i>post hoc</i> test (*p<0.05 and **p<0.01 <i>vs</i>. control, #p<0.05 and ##p<0.01 <i>vs</i>. TAA group in the same region). For details see caption for Figs <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0134434#pone.0134434.g001" target="_blank">1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0134434#pone.0134434.g002" target="_blank">2</a>.</p

The effects of finasteride (FIN) and thioacetamide (TAA) on expression of cytosolic (SOD1) and mitochondrial SOD izoenzyme (SOD2) in the (a) cortex and (b) the hippocampus.

<p>For determination of expression polyclonal goat anti-SOD1 (1:500) and polyclonal goat anti-SOD2 (1:500) were used. After incubation with primary antibodies the membranes were incubated with the horseradish peroxidase (HRP) labeled secondary anti-goat antibody (1:2000) in TBST for 1 hour at room temperature. Five subsequent washes with 0.1% TBST were performed between each step. All membranes were stripped and re-probed with mouse monoclonal anti-actin antibodies (1:10000) to ensure that all wells were equally loaded. The signal was detected by enhanced chemiluminescence and subsequent exposure on an X-ray film. The significance of the difference was estimated by ANOVA with Fisher’s <i>post hoc</i> test (*p<0.05 and **p<0.01 <i>vs</i>. control, ##p<0.01 <i>vs</i>. TAA group, †p<0.01 <i>vs</i>. FIN group). For details see caption for <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0134434#pone.0134434.g001" target="_blank">Fig 1</a>.</p

The effect of finasteride (FIN) and thioacetamide (TAA) on plasma ammonia concentration.

<p>Daily doses of FIN (50 mg/kg) and TAA (300 mg/kg) were administered intraperitoneally in three subsequent days and in FIN+TAA group FIN was administered 2 h before every dose of TAA. Blood samples were collected from the right side of the heart 24 h after administration of the last dose of TAA. The significance of the difference was estimated by ANOVA with Fisher’s <i>post hoc</i> test (**p<0.01 <i>vs</i>. control).</p