Identifikasi Boraks, Formalin dan Kandungan Gizi Serta Nilai Tipe pada Bakso yang Dijual di Lingkungan Perguruan Tinggi di Kota Kupang

Penelitian ini bertujuan untuk mengidentifikasi penambahan boraks, formalin dan uji kandungan gizi serta TPC pada bakso daging sapi yang dijual di Lingkungan perguruan tinggi di Kota Kupang. Boraks dan formalin merupakan agen anti bakteri yang dapat menghambat pertumbuhan bakteri, dan apabila dicampurkan kedalam makanan dapat berbahaya bagi kesehatan karena boraks dan formalin bersifat karsinogenik. Lokasi pengambilan sampel dilakukan di kantin Universitas di Kota Kupang yaitu kantin Undana (2), kantin Unwira (3), kantin Politani (2) dan kantin PGRI (1), total sampel pengujian yaitu 8 sampel. Pengujian boraks dan formalin dilakukan pada laboratorium FKH Undana, dan diidentifikasikan menggunakan kit Easy Test®, pengujian kandungan gizi total protein kasar dan lemak kasar dilakukan di laboratorium Kimia dan Pakan Fakultas Peternakan Undana, serta pengujian TPC dilakukan pada laboratorium Bioreproduksi dan Kesehatan Ternak Fakultas Peternakan Undana. Hasil penelitian menunjukkan negatif untuk identifikasi boraks dan formalin. Berdasarkan SNI 01-3818-1995, standar yang ditetapkan untuk kandungan protein adalah minimal 9,0%, dan lemak maksimal 2,0%. Hasil pengujian protein menunjukkan 3 dari 8 sampel atau 37,5% sampel yang tidak memenuhi standar SNI. Hasil pengujian lemak terdapat 5 dari 8 sampel atau 62,5% sampel yang melebihi batas maksimum SNI. Berdasarkan SNI 01-3818-1995 batas cemaran mikroba pada bakso adalah 1x10³, dan pada hasil penelitian ini didapatkan bahwa terdapat 3 sampel dari 8 sampel atau sebanyak 37,5% sampel yang melebihi batas cemaran maksimum. Dari data diatas dapat disimpulkan, 2 dari 8 sampel atau 25% dari total sampel bakso daging sapi yang dijual di Lingkungan perguruang Tinggi di Kota Kupang aman dari bahaya boraks, formalin, dan kandungan gizi serta bahaya mikroorganisme

Pengaruh Pemberian Ekstrak Etanol Daun Sambiloto( Andrographis Paniculata Nees) terhadap Histopatologi Pankreas Mencit ( Mus Musculus ) Diabetes Melitus (DM) Tipe I

Diabetes mellitus (DM) is a metabolic disorder which damage the beta cells (β) which led to the destruction of langerhans of pancreas gland. Diabetes mellitus is characterized by increased blood glucose levels and progressive changes to the structure of the histopathology of the pancreas. The purpose of this study was to determine the effect of ethanol extract of sambiloto leaf ( Andrographis paniculata Nees) on pancreatic histopathology of Alloxan induced diabetic mice. This research is an experimental research design by Post Test Randomized Control Group Design Experim ental animals used were mice ( Mus musculus) 12 sample of mice ±2 weeks old were adapted for 5 days. Mice were divided into 4 groups, namely, negative control, positive contro l (3 days of alloxan injection) and diabetic mice treated with the ethanol extract of sambiloto leaf with 2 different doses (doses I =2,2mg/kg and dosesII=4,4mg/kg) for 14 days. On day15, mice were necropsy and pancreas were collected for further Hematoxiline Eosin (HE) coloration. The results showed that DM alloxan induced type 1 mice (Mus  musclus), the pancreas characterized by the occurrence of necrosis, langerhans islet was not bounded clear and breakthe linkage of the langerhans islet with a Cinar while on ethanol extract therapy of sambiloto leaf dose 2,2 mg/kg and 4,4 mg/kg in mice DM type I showed the results an improvement on the langerhans islet. Based on these results, it can be concluded that a dose of 2,2 mg/kg and 4,4 mg/kg is effective dose to repair the structure of langerhans islet of mice Type I DM, but it is more adv isable to us a therapeutic dose of 4,4 mg/kg because it changes the structure of Langerhans islet

Efek Pemaparan Deltamethrin pada Broiler terhadap Aktivitas Enzim Alanin Aminotransferase, Aspartat Aminotransferase dan Gambaran Histopatologi Hepar

The purpose of this study is to determine the effects of deltamethrin exposure on the Broiler\u27s liver histopathological feature, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) enzyme activity. Fourty DOC Broilers strain New Loghman are divided into four group of ten and they were adapted for 5 days prior to the treatment. Group I (KI) is a control group, group II were given 20 mg/L deltamethrin, group III were given 10 mg/L deltamethrin and exposure deltamethrin concentration 10 mg/L and group IV were given 5 mg/L delthametrin. Deltamethrin was mixed with drinking water and then was given to the treatment group for 30 days. Blood samples were taken on day 0, day 15 and day 30 of treatment to determine of ALT and AST enzyme activity. On day 35, all animal were sacrificed, liver were taken out and fixed in 10% of buffer formalin for microscopic examination. Results of the AST enzyme activity shows that exposure to 20 mg/L and 10 mg/L deltamethrin for 30 days resulted in the histopathological changes of the liver, such as, fatty degeneration, necrosis on liver cells, inflamation of the liver, and necrosis on liver cells without the infiltration of inflamation cells. It is conclude that exposure to 20 mg/L deltamethrin for 30 days resulted in an increase in AST enzyme activity which is supported liver histopathological changes: fatty degeneration, necrosis, inflamation, and necrosis on liver cells without the infiltration of inflamation cells

Identifikasi Kandungan Logam Berat Kadmium (Cd) pada Sapi Potong yang Dipelihara di Tempat Pembuangan Akhir (Tpa) Kecamatan Alak, Kota Kupang

One of m ajor obstacle for cattle breeding enterprise in an urban area called Kupang city is the limited availability of grazing land . Attempting to overcome this , farmers in Kupang to utilize Alak final disposal (landfill) WITHOUT graz land of Alak. The purpose of this research was to identify the content of heavy metal cadmium (Cd) in cattle reared on waste/final disposal in Alak, Kupang . 12 blood samples were taken on the basis of various criteria , namely from the age group of 0 to 1.5 years of age; age group of 1.5 to 3 years and a group of more than 3 years. 3 ml blood samples were determined/ were measured by atomic absorption spectrophotometry in the Quality Testing Center and Animal Product Certification in Bogor. Results showed that the highest number was 0 .0297 ug/ml in the age group of 0 to 1.5 years) and the lowest was 0.0170 ug/ml in the age group over 3 years. Results showed that the highest number was 0.0297 ug/ml in the age group of 0 to 1.5 years) and the lowest was 0.0170 ug/ml in the age group ove r 3 years. In conclusion, 100% of heavy metal Cadmium was accumulated in beef cattle raised on Alak landfills and it might harmful to be consumed by people who live in kupang

Efektivitas Bakteri Asam Laktat yang Diisolasi dari Susu Kuda Sumba terhadap Kualitas Silase Jerami Padi

This study aims to evaluate the ability of lactic acid bacteria isolated from Sumba horse milk as a starter in improving the quality of rice straw silage. Determination of the quality of rice straw silage through organoleptic tests, measurement of percentage of silage damage and silage pH. there are 7 treatments based on silage compositions and lactic acid bacteria. The results showed the presence of golden color in all treatments as the original color, the texture was very soft to soft and not slimy, and the presence of a distinctive silage odor or close to the typical smell of silage in all treatments. The presentation of silage damage to rice straw in the positive control group showed the highest percentage of damage that was equal to 43.58%, while the percentage of damage in the treatment group ranged from 28.57% to 41.02%. The pH results showed the control group had a pH of 3.6 and the P1-P6 group had an average pH ranging from 5.7 to 6.5. The addition of lactic acid bacteria in the manufacture of silage in rice straw provides good quality silage results

Uji Potensi Antibakteri Ekstrak Etanol Daun Kayu Metang (Lunasia Amara Blanco) terhadap Staphylococcus Aureus

Staphylococcus aureus is a normal flora in living things found on the skin and mucous membranes. Staphylococcus aureus can potentially cause infections of the skin and mammary glands. Natural ingredients that have the potential as antibacterial are Kayu Metang Leaves (Lunasia amara blanco) Manggarai people use the leaves to treat diabetes and wounds on the skin. The purpose of this study was to determine the secondary metabolites contained in Kayu Metang leaves and to determine the level of effective concentration as an antibacterial Staphylococcus aureus. This study used four replications and there were three variations of the ethanol extract concentration, namely 5%, 10%, and 20%. Data analysis in this study employed One-Way Anova followed by normality test and homogeneity test with 95% confidence level (a = 0.05). The results revealed that the inhibition zones resulting from concentrations of 5%, 10%, and 20% had different diameter values ​​and had the same average antibacterial strength, namely the strong category because the range of inhibition zones formed from 9.525 mm to 14.65 mm. The result of the one-way Anova test was a significance of p = 0.397. There was no significant difference in each concentration of metang wood leaf extract with a p value> 0.05. The use of metang wood extract had a smaller inhibitory power than the use of the antibiotic ciprofloxacin, but the use of metang wood extract has been shown to inhibit S. aureus bacteria. This study concluded that the leaves of metang wood (Lunasia amara blanco) have been shown to contain alkaloids, flavonoids, saponins, steroids and triterpenoids, and antibacterial compounds to the growth of Staphylococcus aureus