Antibacterial Activity of Some Household Surface Cleaners against Common Pathogens

Surface cleaner has been widely used to control infections and its transmission. In Hospitals Antiseptics are used to control microbial growth on living tissues and inanimate objects. The activity of six commercial household surface cleaner, Dettol, Phenyl, Harpic, Max, sweepy and local surface cleaner were tested against common pathogens of 10 different species of each strains of Staphylococcus aureus, Escherichia coli, Pseudomonas aeroginosa and Bacillus subtilis. Disc diffusion method were implanted with different concentrations of 100%,75%,50%,25% for each surface cleaner and applied on MHA. After incubation of 24 hours inhibition zones were measured as the maximum zone of inhibition were observed by Staphylococcus aureus at 75 % concentration and the minimum zone of inhibition were observed by Bacillus subtilis at 25 % concentrations. This conducted study showed effectively killing of Disinfectant in all test organisms and provide greatest protection in the transmission of diseases

Association patterns of Atrial Fibrillation with symptoms of metabolic syndrome

Background: Atrial fibrillation (AF) is the common cardiac arrhythmia in which heart beats irregularly usually greater than 100 beats per minutes. AF is well-documented public health problem causing substantial mortality and morbidity. Metabolic syndrome (MS) is a collection of metabolic risk factors like diabetes, hypertension, dyslipidemia, obesity and impaired glucose level that exists in one person. The aim of the present study is to find the relation between AF and MS.Methods: 100 patients of AF were sampled from Punjab Institute of Cardiology, Lahore from December, 2014 to June 2015. These patients were divided into two AF groups, 50 without MS and 50 with MS. 25 healthy subjects were also included for the comparison. Lipid profile was assessed by chemistry analyzer and serum insulin was measured by ELISA.Results: In our population, there was significantly high levels of insulin resistance (IR) and obesity in AF groups (without MS and with MS) as compared with healthy subjects (P<0.05). Highly significant differences was observed in relation with other parameters e.g. hypertension, hypertriglyceridemia, HDL-C among the studied groups (P<0.05). Significant positive correlation was observed between insulin and lipid profile (TC, TG, LDL) while inverse with HDL.Conclusion: The indictors of metabolic syndrome were significantly correlated with AF in the studied subjects, while IR was found significantly higher in MS group.

Artificially remediated plants impact soil physiochemical properties along the riparian zones of the three gorges dam in China

River ecosystem biodiversity and biogeochemical processes are shaped largely by riverside vegetation and soil. Moreover, river ecosystems provide ecological services influenced by the surrounding vegetation and soil interactions. However, the mechanisms by which artificially remediated plants (ARPs) and riparian soil interact to provide these benefits are still unclear among various ARPs. This study fills this gap and examines the impact of ARPs along the riparian zones of Three Gorges Dam (TGD) in Chongqing City, China. We sampled four varieties of ARPs from the Ruxi River Basin in the TGD. These varieties included Cynodon dactylon, Hemarthria altissima, Taxodium disticum, and Salix mastudana. Our results indicated substantial changes in soil physicochemical parameters. Comparably, T. distigum contains significantly higher soil chemical contents. Interestingly, principal component analysis explained almost 100% of the variance for all plant species in this study. Moreover, different vegetation types and soil chemical properties were positively correlated using Pearson correlation analysis (p &lt; 0.05). Furthermore, all plant species exhibited strong negative correlations with physical characteristics (up to r = −1.00). Specifically, these mechanisms explain the interactions between ARPs and soil from riparian areas in the TGD. Hence, this study may facilitate ecological restoration and land management in degraded riparian areas

Maternal and peri-natal outcomes of dengue fever with special emphasis on vertical transmission

Objectives: To investigate the maternal and peri-natal outcomes of dengue infection and frequency of dengue immunoglobulin G positivity in pregnant women along with pregnancy outcomes. Method: The observational two-phase study was conducted from 2012 to 2015 in Lahore, Pakistan. I In phase 1, pregnant women who had acute dengue fever were prospectively and retrospectively enrolled from 4 tertiary care hospitals. Demographic data, clinical/laboratory parameters and maternal/foetal outcomes were recorded for each subject. In the second phase, normal pregnant women with no current or past history of dengue fever were enrolled from Gynaecology Department of Shaikh Zayed Hospital, Lahore, to determine the frequency of dengue immunoglobulin G positivity and pregnancy outcome. Data was analysed using SPSS 20. ---Continu

Carbazole Alkaloids from Stem Bark of Murraya koenigii (L.) Spreng

Murraya koenigii (L.) Spreng(curry patta) has different therapeutic uses and rich source of carbazole alkaloids. Phytochemical studies on the stem bark of M. koenigii yielded one new carbazole alkaloid, afifine, along with two known carbazole alkaloids, mahinimbine and girinimbine. These compounds were isolated using chromatographic methods and identified using spectroscopic techniques

Carbazole Alkaloids from Stem Bark of Murraya koenigii (L.) Spreng

Murraya koenigii (L.) Spreng(curry patta) has different therapeutic uses and rich source of carbazole alkaloids. Phytochemical studies on the stem bark of M. koenigii yielded one new carbazole alkaloid, afifine, along with two known carbazole alkaloids, mahinimbine and girinimbine. These compounds were isolated using chromatographic methods and identified using spectroscopic techniques

Association of adiponectin gene expression with atrial fibrillation in a Pakistani populace

Abstract Adiponectin, an adipocytokine produced and secreted by adipose tissue, has anti-diabetic, anti-atherogenic, and anti-inflammatory properties. This case-control study was aimed to assess the expression and serum levels of adiponectin in subject suffereing from atrial fibrillation (AF). The study's subjects (n = 690) were enrolled from the Punjab Institute of Cardiology, Lahore and were grouped into control, AF without Metabolic syndrome (MetS), and AF with MetS groups. Along with the collection of demographic data, an analysis of adiponectin and biochemical parameters were performed. A highly significant difference in serum levels of adiponectin was observed among the control, AF without MetS, and AF with MetS groups (61.61 ± 45.30 ng/ml, 37.20 ± 19.46 ng/ml, 63.78 ± 61.69 ng/ml). The expression analysis of adiponectin was decreased (n-fold = ̴ 0.30) in AF without MetS group as compared to control group (n-fold =  ~ 1.16) but increased in AF with MetS group (n-fold = ̴ 6.26). The correlation analysis revealed a highly significant positive relationship between the expression of the adiponectin gene with waist-to-hip ratio (WHR) in AF without MetS group. Whereas, serum adiponectin was negatively related to serum triglycerides (TG) in AF with MetS group. In multiple regression analysis using adiponectin expression as the dependent variable, WHR was a determinant in AF without MetS. Whereas, when serum adiponectin was used as the dependent variable, serum TG was the determinant in group AF with MetS. The present study implicates that decreased expression and serum levels of adiponectin were associated with the development of AF in which WHR and serum TG also contributed towards the onset of atrial fibrillation

Identification, pyramid and candidate genes of QTLs for associated traits based on a dense erect panicle rice CSSL-Z749 and five SSSLs, three DSSLs and one TSSL

Abstract Background Seed-set density is an important agronomic trait in rice. However, its genetic mechanism is complex. Chromosome segment substitution lines (CSSLs) are ideal materials for studying complex traits. Results A rice CSSL, Z749, with a dense and erect panicle phenotype, was identified among progeny of the recipient parent Nipponbare and the donor parent Xihui 18. Z749 carried seven substitution segments (average length 2.12 Mb). Compared with Nipponbare, Z749 showed significant increases in the numbers of primary (NPB) and secondary branches (NSB), number of spikelets (SPP) and grains per panicle (GPP), seed-set density (SSD), and decrease in panicle length (PL). A secondary F2 population derived from a cross between Nipponbare and Z749 was used to map quantitative trait loci (QTLs) for associated traits. Fifteen QTLs distributed on chromosomes 5, 7, 8, and 10 were detected. The QTL qPL7 might be an allele of OsFAD8 and the remaining 14 QTLs (e.g., qSSD5 and qSSD10 etc.) might be novel. Fourteen QTLs were verified using five single-segment substitution lines (SSSLs). The seed-set density of Z749 was controlled predominantly by one major QTL (qSSD10) and two minor QTLs (qSSD5 and qSSD8). The QTLs qSSD10, qSSD5, and qSSD8 were fine-mapped to intervals of 1.05, 1.46, and 1.53 Mb on chromosomes 10, 5, and 8, respectively. Analysis of QTL additive effects indicated that qSSD5, qSSD8, and qSSD10 from Xihui18 increased seed-set density of Z749 by 14.10, 11.38, and 5.11 spikelets per 10 cm panicle, respectively. Analysis of QTL epistatic effects revealed that pyramiding of qSSD5 and qSSD8, qSSD5 and qSSD10, qSSD8 and qSSD10, and qSSD5, qSSD8 and qSSD10 produced novel genotypes with increased seed-set density. Conclusions Inheritance of seed-set density in Z749 was controlled predominantly by one major QTL (qSSD10) and two minor QTLs (qSSD5 and qSSD8). Then, they were fine-mapped to intervals of 1.05, 1.46, and 1.53 Mb on chromosomes 10, 5, 8, respectively. Two MAPK genes (OsMPK9 and OsMPK17) and one gene (candidate gene 6) involved in auxin metabolism might be candidate genes for qSSD5, and OsSAUR32 might be the candidate gene for qSSD8. Pyramiding of qSSD5, qSSD8, and qSSD10 enhanced seed-set density