Dividend Policy and Firm Performance with Moderating Effect of Ownership Structure: Evidence from The Manufacturing Firms in Pakistan

The objective of this paper is to check the impact of dividend policy and firm performance with a moderating effect of ownership structure. Many studies have been made to check the impact of dividend policy on firm performance but there is not a single study that has been made yet to check the impact of dividend policy and firm performance with moderating effect of ownership structure. This research had been carried out on different non-financial sectors of Pakistan covering the firms of automobile assembler and parts manufacturing, cement and chemical manufacturing, fertilizer manufacturing, pharmaceuticals, and textile manufacturing etc. The data used in carrying out this research was taken from the companies that was paying dividends and were listen in KSE-100 index of Pakistan Stock Exchange. Ownership data of the firms were collected by the company’s pattern of shareholding which are published or mentioned in the company’s annual report as per Securities and Exchange Commission of Pakistan (SECP) requirements that can be taken from the company’s website. The study shows significant positive relationship between dividend policy and firm performance. The study also shows that there is positive significant relationship between dividend policy and firm performance with moderating effect of ownership structure. Dividend policy can also have implications for a company\u27s investment and growth prospects. In general, firms that retain more of their earnings for reinvestment are likely to have higher levels of capital expenditures and research and development spending. However, dividend payments can also signal to investors that a company has confidence in its future growth prospects

Sequence Diversity of MAOA Gene within Wild and Docile Animal Species

Background: Molecular characterization of MAOA gene was performed to investigate aggressive behaviour within wild (lion, leopard, and wolf) as opposed to docile animal (sheep, goat) species living in different habitats, by undertaking sequence diversity analysis of this gene.Methods: The MAOA gene was partially amplified by PCR for wild and docile animal species. Amplified DNA was sequenced and then analyzed using BioEdit and Sequencher softwares, while multiple sequence alignment and phylogenetics analysis were conducted through MEGA software. Bioinformatics tool like Prosite scan, Motif Scan and Prot Param were used to study properties of mutant proteins of MAOA gene.Results: Different polymorphic sites were observed which included c.956, c.1063 in docile animals and c.2530 in wild animals. Phylogenetic analysis based on this candidate gene endorsed the existing taxonomy of subject animals, while bioinformatics tools explored the altered characteristics of mutant MAOA protein.   Conclusion: The newly found polymorphic loci in wild and docile animals in this study could have a role in behavioral response and acclimatization within their peculiar habitats. This study also highlights the genetic diversity of MAOA gene, which will add knowledge to the existing animal genetic resource of Pakistan.

Molecular Characterization of EGLN1 Gene in Fast and Slow Moving Animals of Diverse Terrain

Background: Process of animal migration from their habitat to a new environment is always problematic due to low adaptive tendency, which ultimately affect their production, behavior and overall performance. Current study is focused to explore the sequence diversity of EGLN1 gene in relation to animal acclimatization at higher altitude under deprived oxygen or ability of better utilization of oxygen which is considered to be liable for their agility in diverse terrains. Fast and slow moving animals of plain and hilly terrains are potential species to study this phenomenon.Methods: Molecular characterization of EGLN1 gene was performed in sheep, goat, buffalo and camels of Pakistan as slow moving candidate species, while tiger, leopard, wolf, ibex, urial and markhor as fast moving candidate species of diverse terrains by extraction their DNA from whole blood, followed by PCR amplification and sequence analysis of EGLN1 gene through BioEdit software. Later on, certain bioinformatics tools like MEGA, protparam and blast2sequence are used for the characterization of the normal and mutant EGLN1 protein.Results: Current study revealed that goat and camel showed a nucleotide change at c.810 position. While all fast moving animals of higher altitude showed a nucleotide change at position c.406 except one sample of markhor.Conclusion: Current study will assist to have an idea of sequence diversity of aforementioned candidate gene responsible for adaption of animals in oxygen deprived environment of diverse terrain and may be responsible for their agile behaviour of movement. Keywords: EGLN1, Fast moving animals, Slow moving animals, Animal acclimatization, Behavioural traits, Pakistani animal

Myeloperoxidase and elastase are only expressed by neutrophils in normal and in inflammed liver

Myeloperoxidase (MPO) is involved in acute and chronic inflammatory diseases. The source of MPO in acute liver diseases is still a matter of debate. Therefore, we analysed MPO-gene expression on sections from normal and acutely damaged [carbon tetrachloride-(CCl4) or whole liver γ-Irradiation] rat liver by immunohistochemistry, real time PCR and Western blot analysis of total RNA and protein. Also total RNA and protein from isolated Kupffer cells, hepatic stellate cells, Hepatocytes, endothelial cells and neutrophil granulocytes (NG) was analysed by real time PCR and Western blot, respectively. Sections of acutely injured human liver were prepared for MPO and CD68 immunofluorescence double staining. In normal rat liver MPO was detected immunohistochemically and by immunofluorescence double staining only in single NG. No MPO was detected in isolated parenchymal and non-parenchymal cell populations of the normal rat liver. In acutely damaged rat liver mRNA of MPO increased 2.8-fold at 24 h after administration of CCl4 and 3.3-fold at 3 h after γ-Irradiation and MPO was detected by immunofluorescence double staining only in elastase (NE) positive NGs but not in macrophages (ED1 or CD68 positive cells). Our results demonstrate that, increased expression of MPO in damaged rat and human liver is due to recruited elastase positive NGs

Changes in gene expression of DOR and other thyroid hormone receptors in rat liver during acute-phase response

Non-thyroidal illness is characterized by low tri-iodothyronine (T3) serum level under acute-phase conditions. We studied hepatic gene expression of the newly identified thyroid hormone receptor (TR) cofactor DOR/TP53INP2 together with TRs in a rat model of aseptic abscesses induced by injecting intramuscular turpentine-oil into each hind limb. A fast (4-6 h) decrease in the serum level of free thyroxine and free T3 was observed. By immunohistology, abundant DOR protein expression was detected in the nuclei of hepatocytes and ED-1+ (mononuclear phagocytes), CK-19+ (biliary cells), and SMA+ (mesenchymal cells of the portal tract) cells. DOR signal was reduced with a minimum at 6-12 h after the acute-phase reaction (APR). Immunohistology also showed a similar pattern of protein expression in TRα1 but without a significant change during APR. Transcripts specific for DOR, nuclear receptor co-repressor 1 (NCoR-1), and TRβ1 were down-regulated with a minimum at 6-12 h, whereas expression for TRα1 and TRα2 was slightly and significantly up-regulated, respectively, with a maximum at 24 h after APR was initiated. In cultured hepatocytes, acute-phase cytokines interleukin-1β (IL-1β) and IL-6 down-regulated DOR and TRβ1 at the mRNA level. Moreover, gene expression of DOR and TRs (TRα1, TRα2, and TRβ1) was up-regulated in hepatocytes by adding T3 to the culture medium; this up-regulation was almost completely blocked by treating the cells with IL-6. Thus, TRβ1, NCoR-1, and the recently identified DOR/TP53INP2 are abundantly expressed and down-regulated in liver cells during APR. Their down-regulation is attributable to the decreased serum level of thyroid hormones and most probably also to the direct action of the main acute-phase cytokines

Comparison of changes in gene expression of transferrin receptor-1 and other iron-regulatory proteins in rat liver and brain during acute-phase response

The “acute phase” is clinically characterized by homeostatic alterations such as somnolence, adinamia, fever, muscular weakness, and leukocytosis. Dramatic changes in iron metabolism are observed under acute-phase conditions. Rats were administered turpentine oil (TO) intramuscularly to induce a sterile abscess and killed at various time points. Tissue iron content in the liver and brain increased progressively after TO administration. Immunohistology revealed an abundant expression of transferrin receptor-1 (TfR1) in the membrane and cytoplasm of the liver cells, in contrast to almost only nuclear expression of TfR1 in brain tissue. The expression of TfR1 increased at the protein and RNA levels in both organs. Gene expression of hepcidin, ferritin-H, iron-regulatory protein-1, and heme oxygenase-1 was also upregulated, whereas that of hemojuvelin, ferroportin-1, and the hemochromatosis gene was significantly downregulated at the same time points in both the brain and the liver at the RNA level. However, in contrast to observations in the liver, gene expression of the main acute-phase cytokine (interleukin-6) in the brain was significantly upregulated. In vitro experiments revealed TfR1 membranous protein expression in the liver cells, whereas nuclear and cytoplasmic TfR1 protein was detectable in brain cells. During the non-bacterial acute phase, iron content in the liver and brain increased together with the expression of TfR1. The iron metabolism proteins were regulated in a way similar to that observed in the liver, possibly by locally produced acute-phase cytokines. The significance of the presence of TfR1 in the nucleus of the brain cells has to be clarified

Women Home Based Workers in Rural areas of Pakistan

Home-based work is a “general category of work within the informal or unorganized sector where workers carry out remunerative activities within their homes or in the surrounding areas but not at the premises of an employer”. The women Home-based workers defines as “The Women who work in their own dwelling or home stations. It is the moneymaking activity. The home based work includes the embroidery, handicrafts, stitching, weaving, shoe making, football making, garments, handmade jewelry and decoration piece making etc. The main purpose of this study was to explore awareness among women home based worker about their rights. The study was conducted in District Faisalabad. Multistage sampling technique was used in this study. At first stage, one Tehsil Faisalabad out of five Tehsils was selected by using simple random sampling technique. At second stage 4 union councils including UC-49, UC-157, and UC-274 were selected by using simple purposive sampling technique. At third stage four villages from each union council out of five villages (Chack No- 225 RB, Malkhawala), (Chack No-217 RB, Chakaira), (Chack No-218 RB, Pronkawala) and (Chack No-61 JB, Dharora) were selected purposively. The study was conducted in rural area of District Faisalabad. The sample size of one twenty women was selected by using simple random sampling.  Questionnaire was used as a tool for data collection and collected data were analyzed by using Statistical Package for Social Sciences (SPSS). Results of the study revealed that 61.7% of the respondents were illiterate, about 38.3% of the respondents earned 2501-5000 rupees, the significant majority 86.7% of the respondents belonged to lower class, about one third 40% of the respondents involved in stitching, more than half 51.7% of the respondents worked up to 6 hours per day, the majority 67.5% the of the respondents had muscular pain, and the significant majority 87.5% of the respondents had no awareness about their rights

A Decade of Mighty Lipophagy: What We Know and What Facts We Need to Know?

From Hindawi via Jisc Publications RouterHistory: publication-year 2021, received 2021-02-22, rev-recd 2021-09-30, accepted 2021-10-15, pub-print 2021-11-05, archival-date 2021-11-05Publication status: PublishedLipids are integral cellular components that act as substrates for energy provision, signaling molecules, and essential constituents of biological membranes along with a variety of other biological functions. Despite their significance, lipid accumulation may result in lipotoxicity, impair autophagy, and lysosomal function that may lead to certain diseases and metabolic syndromes like obesity and even cell death. Therefore, these lipids are continuously recycled and redistributed by the process of selective autophagy specifically termed as lipophagy. This selective form of autophagy employs lysosomes for the maintenance of cellular lipid homeostasis. In this review, we have reviewed the current literature about how lipid droplets (LDs) are recruited towards lysosomes, cross-talk between a variety of autophagy receptors present on LD surface and lysosomes, and lipid hydrolysis by lysosomal enzymes. In addition to it, we have tried to answer most of the possible questions related to lipophagy regulation at different levels. Moreover, in the last part of this review, we have discussed some of the pathological states due to the accumulation of these LDs and their possible treatments under the light of currently available findings

A Comparison of Re-Sampling Techniques for Detection of Multi-Step Attacks on Deep Learning Models

The increasing dependence on data analytics and artificial intelligence (AI) methodologies across various domains has prompted the emergence of apprehensions over data security and integrity. There exists a consensus among scholars and experts that the identification and mitigation of Multi-step attacks pose significant challenges due to the intricate nature of the diverse approaches utilized. This study aims to address the issue of imbalanced datasets within the domain of Multi-step attack detection. To achieve this objective, the research explores three distinct re-sampling strategies, namely over-sampling, under-sampling, and hybrid re-sampling techniques. The study offers a comprehensive assessment of several re-sampling techniques utilized in the detection of Multi-step attacks on deep learning (DL) models. The efficacy of the solution is evaluated using a Multi-step cyber attack dataset that emulates attacks across six attack classes. Furthermore, the performance of several re-sampling approaches with numerous traditional machine learning (ML) and deep learning (DL) models are compared, based on performance metrics such as accuracy, precision, recall, F-1 score, and G-mean. In contrast to preliminary studies, the research focuses on Multi-step attack detection. The results indicate that the combination of Convolutional Neural Networks (CNN) with Deep Belief Networks (DBN), Long Short-Term Memory (LSTM), and Recurrent Neural Networks (RNN) provides optimal results as compared to standalone ML/DL models. Moreover, the results also depict that SMOTEENN, a hybrid re-sampling technique, demonstrates superior effectiveness in enhancing detection performance across various models and evaluation metrics. The findings indicate the significance of appropriate re-sampling techniques to improve the efficacy of Multi-step attack detection on DL models

Universal Hints for an All Round Development of Human Personality: Vladimir Lenin as a Case Study 1

The aim of this study is to investigate universal hints for an all-round development of human personality: Vladimir Lenin as a case study, in the light of Stephen R. Covey's suggested habits, expounded in his books, "The Seven Habits of Highly Effective People" and "The 8th Habit: From Effectiveness to greatness", following the most eminent Russian physiologist and psychologist I. P. Pavlov's theory of classical behaviourism. This paper adopts the popped up chunks of I. P. Pavlov's classical behaviourist theory to analyse how the process of habit formation influences the effective and great human personalities of the world. Thus, the present study will enable the readers and researchers to confront Pavlov's classical behaviourist theory of habit formation through conditioned and unconditioned stimuli and reflexes. Readers are also expected to abandon the bad habits and adopt the good ones. Through infrequent but subtle universal hints which will serve as a model of effective and great human personality of the world. Applying I. P. Pavlov's classical behaviourist theory, this paper concentrates on all round development of gallivanting and history making personality of Vladimir Lenin as a case study, who identified himself with history, and present Vladimir Lenin in novel and innovative perspective