In vitro analysis of the effect of Go Ark on Human Peripheral Blood Lymphocytes

Cow is worshiped in India as “Gomata” since ancient time. Its values have been signified in Vedas, Puranas & Ayurveda. Its urine/Go Ark is used in rituals & medicines traditionally in India. The Significance of Cow Urine has been studied by many workers. Now it is available in the market as distillate. Hence this study was designed to assess the potential of Fresh Go Ark (FGA) and Distillate Go Ark (DGA) on Human Peripheral blood lymphocytes (PBL) in Vitro using MTT Assay. It was found that FGA & DGA both had the potential to enhance the cell viability of Human PBL. FGA showed greater potential towards the enhancement of cell viability on Human PBL than that of DGA. However the difference between the impacts of FGA & DGA was not found to be significant when tested through Two way ANOVA

Go Ark; an ameliorative bio-product (in vitro) on Phenyl induced cytotoxicity

Phenyl (PHY) is one of the chemicals which are used as a disinfectant in the world due to its toxic potential. Cleaning workers are directly exposed to it in institutes, hospitals and houses. Cow urine/Go Ark (GA) has been proved as a bioenhancer in many studies. The present study dealt with the in vitro analysis of PHY induced cytotoxicity (CT) on human peripheral blood lymphocytes and ameliorative potential of Distillate cow urine/Go Ark (DGA) and Fresh Go Ark (FGA) as GA is believed to be an elixir in Ayurved. MTT assay was used to study CT and Cell viability % on Human peripheral blood lymphocytes (HPBL) in vitro. CT of PHY was found to be higher than that of DGA and FGA treated groups. This showed that when PHY induced cells were treated with DGA and FGA, they showed increase in the cell viability %. It was also found that FGA had more potential for enhancing cell viability % of HPBL than that of DGA. We suggest that GA can be used as an ameliorative agent on PHY induced CT. It can be explored by in vivo experiments further for its detoxification properties. Now a day, PHY is used in combination with GA for cleaning purposes as “Gonyl”, it may be safe for cleaning workers to use GA based disinfectants to diminish the CT induced due to PHY exposure at the time of cleaning