PREVENTION OF FALL IN PLATELET COUNT BY CARICA PAPAYA LEAF JUICE IN CARBOPLATIN INDUCED THROMBOCYTOPAENIA IN MICE

ABSTRACT Background: There are no effective methods to treat thrombocytopenia once it occurs. Transfusions, growth factor injections and bone marrow transplant have their limitations. So there is increased need for research of drugs that could prevent and treat thrombocytopenia. The objective the study to determine the effect of different doses of male and female papaya leaf juice on prevention of carboplatin induced thrombocytopenia in mice. Methods: A total of 55 Swiss albino mice were randomly divided into five groups (C, M10, M5, F10 and F5). Thrombocytopaenia was induced in all groups by a single intraperitoneal injection of carboplatin. Male papaya leaf juice was given to prevent of thrombocytopaenia to groups M10 and M5 and female papaya leaf juice was given to F10 and F5. On days 0, 7, 14 and 21 blood samples were collected by cardiac puncture for platelet count. Significance of difference was calculated by one way ANOVA. Results: After carboplatin injection, platelet count decreased. Papaya leaf juice prevented fall in platelet count throughout the study period with p-value < 0.001. Difference between male and female papaya leaf juice was not significant while higher dose (10 ml/kg) produced significantly higher responses as compared to low dose (5 ml/kg). Conclusion: Papaya leaf juice prevents reversible thrombocytopaenia induced by carboplatin in a dose dependent manner. There is no difference between male and female plants in this respect

Multidisciplinary Management and Outcome of Intradural Extramedullary Spinal Tumors

Introduction/Objective:  About fifteen percent of the primary CNS tumors are intraspinal. About two-thirds of tumors are intradural extramedullary (IDEM). This study was conducted to review the outcome of operative management of intradural extramedullary tumors in correlation with the factors, both clinical & histopathological, influencing the neurology of patients & prognosis. Materials and Methods:  It was a multicenter study including 42 patients conducted from December 2018 to December 2020. All patients were diagnosed by MRI with and without contrast. Patients were surgically treated & analyzed for clinical features i.e., pain by visual analog scale (VAS) & neurology by modified McCormick scale both preoperatively & post-operatively. Clinical features & outcomes were correlated with tumor size & histopathology. p-value < 0.05 was considered significant. Results:  This study included 42 cases. The most common diagnosis was schwannoma (76.19%). The average intradural space occupied at presentation was 82%. The most common location was dorsal (90.4%). The visual analog score for pain (VAS) improved in all patients post-operatively from 7 ± 1.9 to 2 ± 0.8 (p = 0.003) & modified McCormick scale from 3.0 ± 1.3 to 2.0 ± 1.0 (p = 0.005). The preoperative symptoms were correlated with the only size of the tumor occupying the intradural space (VAS p = 0.021, modified McCormick scale p = 0.018). Conclusion:  All the tumors excised showed some improvement in neurological status. Therefore, all patients diagnosed with IDEM should be operated on even if present with prolonged symptoms or severe neurological compromise. Keywords:  Intradural Extramedullary, Meningioma, Schwannoma, Intraspinal