Characterization of extracellular amylase enzyme produced by <i>Aspergillus flavus</i> MV5 isolated from mangrove sediment

170-173Mangroves provide a unique ecological niche to different microbes which play various roles in nutrient recycling as well as various environmental activities. Analysis of microbial biodiversity from these ecosystems will help in isolating and identifying new and potential microorganisms having high specificity for various applications. Mangroves sediment soils are a resource of fungi providing an end number of enzymes that finds their use in industrial processes. This study indicates the extracellular production of amylase by Aspergillus flavus MV5 was confirmed on GYP agar plates. Further maximum enzyme activity was optimized and the DNA of fungal strains were isolated to sequence the ITS region of 5.8s rRNA with an ITS primer. The novelty of the strain was checked by a BLAST analysis for submission to GenBank