MOESM5 of Blocking ERK1/2 signaling impairs TGF-β1 tumor promoting function but enhances its tumor suppressing role in intrahepatic cholangiocarcinoma cells

Additional file 5. Steady state levels of TGF-β1, activin and nodal expression in KKU-M213 cells. Levels of TGF-β1, activin and nodal mRNA were determined by SYBR-green-based qRT-PCR using RNA extracted from 80% confluent cells cultured in 10% FBS media. Relative mRNA levels were calculated using 2−ΔCt formula compared to that of 18 s rRNA