271 research outputs found

    Formation of Galactic Center Magnetic Loops

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    A survey for the molecular clouds in the Galaxy with NANTEN mm telescope has discovered molecular loops in the Galactic center region. The loops show monotonic gradients of the line of sight velocity along the loops and the large velocity dispersions towards their foot points. It is suggested that these loops are explained in terms of the buoyant rise of magnetic loops due to the Parker instability. We have carried out global three-dimensional magneto-hydrodynamic simulations of the gas disk in the Galactic center. The gravitational potential is approximated by the axisymmetric potential proposed by Miyamoto & Nagai (1975). At the initial state, we assume a warm (~ 10^4 K) gas torus threaded by azimuthal magnetic fields. Self-gravity and radiative cooling of the gas are ignored. We found that buoyantly rising magnetic loops are formed above the differentially rotating, magnetically turbulent disk. By analyzing the results of global MHD simulations, we have identified individual loops, about 180 in the upper half of the disk, and studied their statistical properties such as their length, width, height, and velocity distributions along the loops. Typical length and height of a loop are 1kpc and 200pc, respectively. The line of sight velocity changes linearly along a loop and shows large dispersions around the foot-points. Numerical results indicate that loops emerge preferentially from the region where magnetic pressure is large. We argue that these properties are consistent with those of the molecular loops discovered by NANTEN.Comment: 16pages, 10figures. Accepted for publication in PASJ. Replace to higher resolution versio

    40Ar-39Ar analysis of phlogopite in the Horoman Peridotite Complex, Hokkaido, Japan and implications for its origin

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    金沢大学理工研究域自然システム学系40Ar-39Ar analysis of phlogopite separated from a plagioclase lherzolite of the Horoman Peridotite Complex, Hokkaido, Japan, has yielded a plateau age of 20.6 ± 0.5 Ma in an environment where the metamorphic fluid was characterized by an almost atmospheric Ar isotopic ratio. The age spectrum is slightly saddle-shaped, implying some incorporation of excess 40Ar during the formation of the phlogopite at a depth. As the phlogopite has been inferred to have formed in veins and/or interstitials during exhumation of the peridotite body, metasomatic fluids, to which ground- and sea water might have contributed, were probably involved in the formation of phlogopite in the crustal environment. A total 40Ar-39Ar age of 129 Ma of a whole rock sample of the plagioclase lherzolite, from which the phlogopite was separated and is representative of the main lithology of the Horoman Peridotite Complex, indicates the occurrence of excess 40Ar. Hence, the age has no geological meaning

    Three novel oligosaccharides synthesized using Thermoanaerobacter brockii kojibiose phosphorylase

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    <p>Abstract</p> <p>Background</p> <p>Recently synthesized novel oligosaccharides have been produced primarily by hydrolases and glycosyltransferases, while phosphorylases have also been subject of few studies. Indeed, phosphorylases are expected to give good results via their reversible reaction. The purpose of this study was to synthesis other novel oligosaccharides using kojibiose phosphorylase.</p> <p>Results</p> <p>Three novel oligosaccharides were synthesized by glucosyltransfer from β-D-glucose 1-phosphate (β-D-G1P) to xylosylfructoside [<it>O</it>-α-D-xylopyranosyl-(1→2)-β-D-fructofuranoside] using <it>Thermoanaerobacter brockii </it>kojibiose phosphorylase. These oligosaccharides were isolated using carbon-Celite column chromatography and preparative high performance liquid chromatography. Gas liquid chromatography analysis of methyl derivatives, MALDI-TOF MS and NMR measurements were used for structural characterisation. The <sup>1</sup>H and <sup>13</sup>C NMR signals of each saccharide were assigned using 2D-NMR including COSY (correlated spectroscopy), HSQC (herteronuclear single quantum coherence), CH<sub>2</sub>-selected E-HSQC (CH<sub>2</sub>-selected Editing-HSQC), HSQC-TOCSY (HSQC-total correlation spectroscopy) and HMBC (heteronuclear multiple bond correlation).</p> <p>Conclusion</p> <p>The structure of three synthesized saccharides were determined, and these oligosaccharides have been identified as <it>O</it>-α-D-glucopyranosyl-(1→2)-<it>O</it>-α-D-xylopyranosyl-(1→2)-β-D-fructofuranoside (saccharide <b>1</b>), <it>O</it>-α-D-glucopyranosyl-(1→2)-<it>O</it>-α-D-glucopyranosyl-(1→2)-<it>O</it>-α-D-xylopyranosyl-(1→2)-β-D-fructofuranoside (saccharide <b>2</b>) and <it>O</it>-α-D-glucopyranosyl-(1→[2-<it>O</it>-α-D-glucopyranosyl-1]<sub>2</sub>→2)-<it>O</it>-α-D-xylopyranosyl-(1→2)-β-D-fructofuranoside (saccharide <b>3</b>).</p

    Effect of three metal priming agents on the bond strength of adhesive resin cement to Ag-Zn-Sn-In alloy and component metals.

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    The aim of this study was to investigate the effect of three metal priming agents on the bond strength of adhesive resin cement to Silver-Zinc-Tin-Indium (Ag-Zn-Sn-In) alloy and pure Ag, Zn, Sn, and In. The specimens were air-abraded with alumina and then primed with one of three metal priming agents: V-Primer, Estenia Opaque Primer, or Alloy Primer. The metal disks were bonded with adhesive resin cement (Super-Bond Bulk-mix technique). Shear bond strengths (n=10/group) were determined before and after 50,000 thermocycles for Ag-Zn-Sn-In alloy as well as after 5,000 thermocycles for pure Ag, Zn, Sn and In. For Ag-Zn-Sn-In alloy, the post-thermocycling bond strength of the Alloy Primer group was significantly higher than that of the other primers. It can be concluded that Alloy Primer containing both the vinyl-thione monomer (VBATDT) and hydrophobic phosphate monomer (MDP) is effective for bonding Ag-Zn-Sn-In alloy and pure Ag, Zn and Sn.福岡歯科大学2017年

    Ycf12 is a core subunit in the photosystem II complex

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    AbstractThe latest crystallographic model of the cyanobacterial photosystem II (PS II) core complex added one transmembrane low molecular weight (LMW) component to the previous model, suggesting the presence of an unknown transmembrane LMW component in PS II. We have investigated the polypeptide composition in highly purified intact PS II core complexes from Thermosynechococcus elongatus, the species which yielded the PS II crystallographic models described above, to identify the unknown component. Using an electrophoresis system specialized for separation of LMW hydrophobic proteins, a novel protein of ∼5 kDa was identified as a PS II component. Its N-terminal amino acid sequence was identical to that of Ycf12. The corresponding gene is known as one of the ycf (hypothetical chloroplast reading frame) genes, ycf12, and is widely conserved in chloroplast and cyanobacterial genomes. Nonetheless, the localization and function of the gene product have never been assigned. Our finding shows, for the first time, that ycf12 is actually expressed as a component of the PS II complex in the cell, revealing that a previously unidentified transmembrane protein exists in the PS II core complex

    Discovery of Molecular Loop 3 in the Galactic Center: Evidence for a Positive-Velocity Magnetically Floated Loop towards L=355359L=355^\circ-359^\circ

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    We have discovered a molecular dome-like feature towards 355l359355^{\circ} \leq l \leq 359^{\circ} and 0b20^{\circ} \leq b \leq 2^{\circ}. The large velocity dispersions of 50--100 km s1^{-1} of this feature are much larger than those in the Galactic disk and indicate that the feature is located in the Galactic center, probably within 1\sim1 kpc of Sgr A^{*}. The distribution has a projected length of 600\sim600 pc and height of 300\sim300 pc from the Galactic disk and shows a large-scale monotonic velocity gradient of 130\sim130 km s 1^{-1} per 600\sim600 pc. The feature is also associated with HI gas having a more continuous spatial and velocity distribution than that of 12^{12}CO. We interpret the feature as a magnetically floated loop similar to loops 1 and 2 and name it "loop 3". Loop 3 is similar to loops 1 and 2 in its height and length but is different from loops 1 and 2 in that the inner part of loop 3 is filled with molecular emission. We have identified two foot points at the both ends of loop 3. HI, 12^{12}CO and 13^{13}CO datasets were used to estimate the total mass and kinetic energy of loop 3 to be \sim3.0 \times 10^{6} \Mo and 1.7×1052\sim1.7 \times 10^{52} ergs. The huge size, velocity dispersions and energy are consistent with the magnetic origin the Parker instability as in case of loops 1 and 2 but is difficult to be explained by multiple stellar explosions. We argue that loop 3 is in an earlier evolutionary phase than loops 1 and 2 based on the inner-filled morphology and the relative weakness of the foot points. This discovery indicates that the western part of the nuclear gas disk of 1\sim1 kpc radius is dominated by the three well-developed magnetically floated loops and suggests that the dynamics of the nuclear gas disk is strongly affected by the magnetic instabilities.Comment: 30 pages, 10 figures. High resolution figures are available at http://www.a.phys.nagoya-u.ac.jp/~motosuji/fujishita09_figs

    A Detailed Observational Study of Molecular Loops 1 and 2 in the Galactic Center

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    Fukui et al. (2006) discovered two huge molecular loops in the Galactic center located in (l, b) ~ (355 deg-359 deg, 0 deg-2 deg) in a large velocity range of -180-40 km s^-1. Following the discovery, we present detailed observational properties of the two loops based on NANTEN 12CO(J=1-0) and 13CO(J=1-0) datasets at 10 pc resolution including a complete set of velocity channel distributions and comparisons with HI and dust emissions as well as with the other broad molecular features. We find new features on smaller scales in the loops including helical distributions in the loop tops and vertical spurs. The loops have counterparts of the HI gas indicating that the loops include atomic gas. The IRAS far infrared emission is also associated with the loops and was used to derive an X-factor of 0.7(+/-0.1){\times}10^20 cm^-2 (K km s^-1)^-1 to convert the 12CO intensity into the total molecular hydrogen column density. From the 12CO, 13CO, H I and dust datasets we estimated the total mass of loops 1 and 2 to be ~1.4 {\times} 106 Msun and ~1.9 {\times} 10^6 Msun, respectively, where the H I mass corresponds to ~10-20% of the total mass and the total kinetic energy of the two loops to be ~10^52 ergs. An analysis of the kinematics of the loops yields that the loops are rotating at ~47 km s-1 and expanding at ~141 km s^-1 at a radius of 670 pc from the center. Fukui et al. (2006) presented a model that the loops are created by the magnetic flotation due to the Parker instability with an estimated magnetic field strength of ~150 {\mu}G. We present comparisons with the recent numerical simulations of the magnetized nuclear disk by Machida et al. (2009) and Takahashi et al. (2009) and show that the theoretical results are in good agreements with the observations. The helical distributions also suggest that some magnetic instability plays a role similarly to the solar helical features.Comment: 40 pages, 22 figures, submitted to publication in PAS

    Similarity between the Molecular Loops in the Galactic Center and the Solar Chromospheric Arch Filaments

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    We carried out two-dimensional magnetohydrodynamic simulations of the Galactic gas disk to show that the dense loop-like structures discovered by the Galactic center molecular cloud survey by NANTEN 4 m telescope can be formed by the buoyant rise of magnetic loops due to the Parker instability. At the initial state, we assumed a gravitationally stratified disk consisting of the cool layer (T103T \sim 10^3 K), warm layer (T104T \sim 10^4 K), and hot layer (T105T \sim 10^5 K). Simulation box is a local part of the disk containing the equatorial plane. The gravitational field is approximated by that of a point mass at the galactic center. The self-gravity, and the effects of the galactic rotation are ignored. Numerical results indicate that the length of the magnetic loops emerging from the disk is determined by the scale height of the hot layer (\sim 100 pc at 1 kpc from the Galactic center). The loop length, velocity gradient along the loops and large velocity dispersions at their foot points are consistent with the NANTEN observations. We also show that the loops become top-heavy when the curvature of the loop is sufficiently small, so that the rising loop accumulates the overlying gas faster than sliding it down along the loop. This mechanism is similar to that of the formation of solar chromospheric arch filaments. The molecular loops emerge from the low temperature layer just like the dark filaments observed in the Hα\alpha image of the emerging flux region of the sun.Comment: 23 pages, 13 figures. Accepted for publication in PAS

    Crucial role of vinexin for keratinocyte migration in vitro and epidermal wound healing in vivo.

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    In the process of tissue injury and repair, epithelial cells rapidly migrate and form epithelial sheets. Vinexin is a cytoplasmic molecule of the integrin-containing cell adhesion complex localized at focal contacts in vitro. Here, we investigated the roles of vinexin in keratinocyte migration in vitro and wound healing in vivo. Vinexin knockdown using siRNA delayed migration of both HaCaT human keratinocytes and A431 epidermoid carcinoma cells in scratch assay but did not affect cell proliferation. Induction of cell migration by scratching the confluent monolayer culture of these cells activated both EGFR and ERK, and their inhibitors AG1478 and U0126 substantially suppressed scratch-induced keratinocyte migration. Vinexin knockdown in these cells inhibited the scratch-induced activation of EGFR, but not that of ERK, suggesting that vinexin promotes cell migration via activation of EGFR. We further generated vinexin (-/-) mice and isolated their keratinocytes. They similarly showed slow migration in scratch assay. Furthermore, vinexin (-/-) mice exhibited a delay in cutaneous wound healing in both the back skin and tail without affecting the proliferation of keratinocytes. Together, these results strongly suggest a crucial role of vinexin in keratinocyte migration in vitro and cutaneous wound healing in vivo
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