1 research outputs found
Self-Assembling DNA Dendrimer for Effective Delivery of Immunostimulatory CpG DNA to Immune Cells
DNA
dendrimers consisting of several branched DNA units connected
to each other using DNA ligase were quite effective for the delivery
of immunostimulatory CpG DNA to immune cells. Therapeutic application
of such DNA dendrimers, however, is hampered by the use of the ligase.
Here, we report that self-assembling DNA dendrimers with high immunostimulatory
potency can be prepared without DNA ligases. Annealing of DNA consisting
of DNA units with elongated adhesive ends resulted in the formation
of DNA dendrimers. Atomic force microscopy revealed that the several
preparations of DNA dendrimers resulted in dendritic structures as
designed. The cellular uptake of DNA dendrimers by mouse macrophage-like
RAW264.7 cells and subsequent release of tumor necrosis factor-α
were dependent on the structural complexity of the dendrimers. These
results indicate that the ligation-free, self-assembling DNA dendrimers
are a potent system for the delivery of immunostimulatory CpG DNA
to immune cells