In vivo development of vitrified COCs from C57BL/6J mouse after in vitro fertilization.

<p>In vivo development of vitrified COCs from C57BL/6J mouse after in vitro fertilization.</p

The effects of cumulus cells on the fertility and developmental ability of vitrified C57BL/6J mouse oocytes after IVF.

<p>IVF was carried out using frozen-thawed BDF1 mouse sperm. Data are means±S.E.M. ^a-b,c-d,e-f Different superscripts denote significant difference (<i>P</i><0.05). In each treatment group, more than 100 oocytes were examined.</p

The effects of cumulus cells on the fertility and developmental ability of C57BL/6J mouse oocytes after IVF.

<p>Data are means±S.E.M. ^a-b,c-d,e-f Different superscripts denote a significant difference (<i>P</i><0.05). In each treatment group, more than 100 oocytes were examined. PN, pronuclear stage; COC, cumulus oocyte complexes; DO, denuded oocytes.</p

The kinetics of COOH-PLL in mouse oocytes.

<p>Mouse oocytes were exposed to PB1(-) supplement with FITC-labeled COOH-PLL (5% (w/v)). Green: FITC-labeled COOH-PLL.</p

The effects of COOH-PLL on survival, fertility and developmental ability of vitrified mouse oocytes after IVF.

<p>Data are shown as means ± S.E.M. Different superscripts denote a significant difference (P<0.05). Numbers of oocytes used in each group were described under the treatment group.</p

Cryoprotectants in equilibration and vitrification medium for mouse oocytes.

<p>*These cryoprotectants were added into calcium free PB1 supplemented with 20% FCS.</p><p>^# These cryoprotectants were added into calcium free PB1 supplemented with 20% FCS and 0.5 M sucrose.</p

In vivo development of mouse oocytes vitrified with COOH-PLL.

<p>In vivo development of mouse oocytes vitrified with COOH-PLL.</p