Root uptake under mismatched distributions of water and nutrients in the root zone

Most plants derive their water and nutrient needs from soils where the resources are often scarce, patchy, and ephemeral. It is not uncommon for plant roots to encounter mismatched patches of water-rich and nutrient-rich regions in natural environments. Such an uneven distribution of resources necessitates plant reliance on strategies for exploring and acquiring nutrients from relatively dry patches. We conducted a laboratory study that elucidates the biophysical mechanisms that enable this adaptation. The roots of tomato (Solanum lycopersicum) seedlings were laterally split and grown in two adjacent, hydraulically disconnected pots, which permitted precise control of water and nutrient applications to each compartment. We observed that the physical separation of water-rich and nutrient-rich compartments (one received 90% water and 0% nutrients and the other received 10% water and 100% nutrients) does not significantly stunt plant growth and productivity compared to two control treatments (control 1: 90% water and 100% nutrients versus 10% water and 0% nutrients; control 2: 50% water and 50% nutrients in each compartment). Specifically, we showed that soil dryness does not reduce nutrient uptake, vegetative growth, flowering, and fruiting compared to control treatments. We identified localized root proliferation in nutrient-rich dry soil patches as a critical strategy that enabled nutrient capture. We observed nocturnal rewetting of the nutrient-rich but dry soil zone (10% water and 100% nutrients) but not in the nutrient-free and dry zone of the control experiment (90% water and 100% nutrients). We interpreted the rewetting as the transfer of water from the wet to dry zones through roots, a process commonly known as hydraulic redistribution (HR). The occurrence of HR likely prevents the nutrient-rich soil from drying due to permanent wilting and the subsequent decline of root functions. Sustaining rhizosphere wetness is also likely to increase nutrient mobility and uptake. Lack of HR in the absence of nutrients suggests that HR is not entirely a passive, water-potential-gradient driven flow. The density and size of root hairs appeared to be higher (qualitative observation) in the nutrientrich and dry compartments than in the nutrient-free and dry compartments. We also observed organic coating on sand grains in the rhizosphere of the nutrient-rich and dry compartments. The observations are consistent with prior observations that root hairs and rhizodeposition aid rhizosphere wetting. These findings were synthesized in a conceptual model that explains how plants of dry regions may be adapted to mismatched resources. This study also suggests that separating the bulk of applied nutrients from the frequently irrigated soil region can increase nutrient use efficiency and curtail water pollution from intensive agricultural systems

Synergy between compost and cover crops in a Mediterranean row crop system leads to increased subsoil carbon storage

Subsoil carbon (C) stocks are a prime target for efforts to increase soil C storage for climate change mitigation. However, subsoil C dynamics are not well understood, especially in soils under long-term intensive agricultural management. We compared subsoil C storage and soil organic matter (SOM) composition in tomato-corn rotations after 25 years of differing C and nutrient management in the California Central Valley: CONV (mineral fertilizer), CONV+WCC (mineral fertilizer and cover crops), and ORG (composted poultry manure and cover crops). The cover crop mix used in these systems is a mix of oat (Avena sativa L.), faba bean (Vicia faba L.), and hairy vetch (Vicia villosa Roth). Our results showed a ∼19Mgha-1 increase in soil organic C (SOC) stocks down to 1m under ORG systems, no significant SOC increases under CONV+WCC or CONV systems, and an increased abundance of carboxyl-rich C in the subsoil (60-100cm) horizons of ORG and CONV+WCC systems. Our results show the potential for increased subsoil C storage with compost and cover crop amendments in tilled agricultural systems and identify potential pathways for increasing C transport and storage in subsoil layers. Copyright

Effect of cover crop on carbon distribution in size and density separated soil aggregates

Increasing soil organic carbon (SOC) stocks in agricultural soils can contribute to stabilizing or even lowering atmospheric greenhouse gas (GHG) concentrations. Cover crop rotation has been shown to increase SOC and provide productivity benefits for agriculture. Here we used a split field design to evaluate the short-term effect of cover crop on SOC distribution and chemistry using a combination of bulk, isotopic, and spectroscopic analyses of size-and density-separated soil aggregates. Macroaggregates (\u3e250 µm) incorporated additional plant material with cover crop as evidenced by more negative δ13C values (−25.4%∘ with cover crop compared to −25.1%∘without cover crop) and increased phenolic (plant-like) resonance in carbon NEXAFS spectra. Iron EXAFS data showed that the Fe pool was composed of 17–21% Fe oxide with the remainder a mix of primary and secondary minerals. Comparison of oxalate and dithionite extractions suggests that cover crop may also increase Fe oxide crystallinity, especially in the dense (\u3e2.4 g cm−3) soil fraction. Cover crop δ13C values were more negative across density fractions of bulk soil, indicating the presence of less processed organic carbon. Although no significant difference was observed in bulk SOC on a mass per mass basis between cover and no cover crop fields after one season, isotopic and spectroscopic data reveal enhanced carbon movement between aggregates in cover crop soil

Effect of Cover Crop on Carbon Distribution in Size and Density Separated Soil Aggregates

Increasing soil organic carbon (SOC) stocks in agricultural soils can contribute to stabilizing or even lowering atmospheric greenhouse gas (GHG) concentrations. Cover crop rotation has been shown to increase SOC and provide productivity benefits for agriculture. Here we used a split field design to evaluate the short-term effect of cover crop on SOC distribution and chemistry using a combination of bulk, isotopic, and spectroscopic analyses of size-and density-separated soil aggregates. Macroaggregates (>250 µm) incorporated additional plant material with cover crop as evidenced by more negative δ13C values (−25.4‰ with cover crop compared to −25.1‰ without cover crop) and increased phenolic (plant-like) resonance in carbon NEXAFS spectra. Iron EXAFS data showed that the Fe pool was composed of 17–21% Fe oxide with the remainder a mix of primary and secondary minerals. Comparison of oxalate and dithionite extractions suggests that cover crop may also increase Fe oxide crystallinity, especially in the dense (>2.4 g cm−3) soil fraction. Cover crop δ13C values were more negative across density fractions of bulk soil, indicating the presence of less processed organic carbon. Although no significant difference was observed in bulk SOC on a mass per mass basis between cover and no cover crop fields after one season, isotopic and spectroscopic data reveal enhanced carbon movement between aggregates in cover crop soil