Feature Matrix for Development of Models for Estimating the Likelihood of PCR Amplification

This data set contains pairs of primers and immunoglobulin heavy chain variable sequences with annotated experimental amplification status according to gel electrophoresis. The data set tabulates the features (e.g. annealing temperature, mismatches) that determine whether a primer leads to the successful amplification of a template

PCR Results for Immunogloublin Heavy Chain Variable Sequences

This data set indicates the amplification status for pairs of primers and immunoglobulin heavy chain variable sequences. CSV files were generated on the basis of raw gel electrophoresis data (XLS file). In the files, cells with an entry of "1" indicate that a template was amplified by the corresponding primer, while a "0" indicates that this was not the case. Measurements were performed in triplicates. Two primer sets were used. Files containing "Tiller" represent primers from Set2, while file containing "Opt" represent primers from set 1. For each primer set, there are five files. Each file represent the labeling of the gel electrophoresis bands by a single person