Additional file 1: of Differential gene expression in response to Fusarium oxysporum infection in resistant and susceptible genotypes of flax (Linum usitatissimum L.)

Genes for which expression was specifically changed in resistant genotypes of flax in response to Fusarium oxysporum infection. (XLSX 9584 kb

Additional file 2: of Differential gene expression in response to Fusarium oxysporum infection in resistant and susceptible genotypes of flax (Linum usitatissimum L.)

Expression profiles of genes for selected gene ontology (GO) terms in susceptible and resistant flax genotypes in response to Fusarium oxysporum infection. (XLSX 861 kb

Principal components biplot on variance stabilized data, color-coded by condition-sex.

<p>PCA scores plot obtained from analysis of gene expression profiles. Proportion of the variance explained is 97.1% for PC1 and 1.1% for PC2.</p

List of unique genes which are up- and down-regulated in both sexes after a particular treatment.

*<p>Gene names were obtained from <a href="http://flybase.org" target="_blank">http://flybase.org</a>.</p>**<p><a href="http://www.uniprot.org" target="_blank">http://www.uniprot.org</a>.</p>***<p>According to Gene Expression Atlas, <a href="http://www.ebi.ac.uk/gxa" target="_blank">http://www.ebi.ac.uk/gxa</a>.</p

Gene-concept networks by gene ontology analysis for toluene treated males and females.

<p><i>Genes, up-regulated in males:</i> Upregulated genes were known to be involved in the protein folding, circadian sleep/wake regulation, positive regulation of transcription from RNA polymerase II promoter and proteolysis regulation. <i>Genes, down-regulated in males:</i> The large number of downregulated genes in this treatment were functionally clustered to four main groups: response to stress (including related functional category of response to heat), response to biotic stimulus, proteolysis and oxidation-reduction process. <i>Genes, up-regulated in females:</i> The overexpressed genes were functionally clustered into four main clusters: oxidation-reduction process, proteolysis, response to stress, response to biotic stimulus and the smaller cluster of cellular response to heat. <i>Genes, down-regulated in females:</i> The cell communication functional category was extremely downregulated in this treatment. Smaller gene clusters, involved in the phototransduction and regulation of response to external stimulus, were also revealed.</p

Gene-concept networks by gene ontology analysis for dioxin treated males and females.

<p><i>Genes, up-regulated in males:</i> Most of upregulated gene the involved in biological regulation, G-protein coupled receptor signaling pathway and regulation of cell differentiation. Smaller functional groups of genes, involved in the regulation of oskar mRNA translation, spermatogenesis, regulation of reproductive process and phospholipase C-activating G-protein coupled receptor signaling pathway were also upregulated. <i>Genes, down-regulated in males:</i> The genes characterized with decreased expression were mostly known to be involved in the cellular respiration and related functional category of mitochondrial ATP synthesis coupled electron transport. Small independent cluster of carboxylic acid metabolic process was also downregulated. <i>Genes, up-regulated in females:</i> Most of genes were annotated as involved in the processes of the proteolysis, defense response, and response to biotic stimulus. The smaller clusters of cellular response to heat and humoral immune response were revealed. <i>Genes, down-regulated in females:</i> The most of downregulated genes were annotated as involved in cell communication. Related functional categories, such as homophilic cell adhesion, were also revealed.</p

Heat map of up-regulated biological processes based on Gene Ontology IDs for treated males (m) and females (fm).

<p>The color scale indicates the number of genes in a group.</p

Diagrams representing the quantity of shared genes between the different treatments.

<p>Diagrams representing the quantity of shared genes between the different treatments.</p

Heat map of molecular pathways based on KEGG analysis IDs for treated males (m) and females (fm), color-coded by enrichment-score.

<p>Heat map of molecular pathways based on KEGG analysis IDs for treated males (m) and females (fm), color-coded by enrichment-score.</p

Relative mRNA level for 8 genes under different influences.

<p>Note: qPCR data. R – relative mRNA level (n-fold increase), in parenthesis a range of mRNA level differences is shown. M – male; F – female.</p