1 research outputs found
Nanopillar Based Enhanced-Fluorescence Detection of Surface-Immobilized Beryllium
The
unique properties associated with beryllium metal ensures the continued
use in many industries despite the documented health and environmental
risks. While engineered safeguards and personal protective equipment
can reduce risks associated with working with the metal, it has been
mandated by the Environmental Protection Agency (EPA) and Occupational
Safety and Health Administration (OSHA) that the workplace air and
surfaces must be monitored for toxic levels. While many methods have
been developed to monitor levels down to the low μg/m<sup>3</sup>, the complexity and expense of these methods have driven the investigation
into alternate methodologies. Herein, we use a combination of the
previously developed fluorescence Be(II) ion detection reagent, 10-hydroxybenzo[h]quinoline
(HBQ), with an optical field enhanced silicon nanopillar array, creating
a new surface immobilized (si-HBQ) platform. The si-HBQ platform allows
the positive control of the reagent for demonstrated reusability and
a pillar diameter based tunable enhancement. Furthermore, native silicon
nanopillars are overcoated with thin layers of porous silicon oxide
to develop an analytical platform capable of a 0.0006 μg/L limit
of detection (LOD) using sub-μL sample volumes. Additionally,
we demonstrate a method to multiplex the introduction of the sample
to the platform, with minimal 5.2% relative standard deviation (RSD)
at 0.1 μg/L, to accommodate the potentially large number of
samples needed to maintain industrial compliance. The minimal sample
and reagent volumes and lack of complex and highly specific instrumentation,
as well as positive control and reusability of traditionally consumable
reagents, create a platform that is accessible and economically advantageous