About Academy of Wrestling Named After D. G. Mindiashvili’s Activities Optimization Methods

В работе рассматриваются типовые проблемы, присущие спортивным сооружениям со специализацией «единоборства», в частности, рассматривается одна из наиболее актуальных – недостаточно рациональное использование площадей и, как следствие, перегруженность объекта. Авторами приведен пример модернизации комплекса, не требующей значительных изменений конструктива, но способствующей повышению пропускной способности, а также в целом качества и спектра оказываемых услугThe work considers typical problems inherent in sports facilities with the specialization of “martial arts,” in particular, one of the most pressing is considered – the insufficiently rational use of space and, as a result, the congestion of the object. The authors give an example of the modernization of the complex that does not require significant changes in the design, but contributes to an increase in throughput, as well as, in general, the quality and range of services provide

Increasing the Attractiveness of Physical Culture and Sports Services in Krasnoyarsk on the Example of Freestyle Wrestling

В данной статье были рассмотрены способы повышения популярности вольной борьбы в г. Красноярске среди молодежи. Для решения поставленной задачи было проведено онлайн-анкетирование среди жителей города с целью выяснения их отношения к занятиям единоборствами. На основе анализа полученных данных были предложены рекомендации по привлечению населения и популяризации занятий по вольной борьбеIn this article there were considered ways to increase popularity of free-style wrestling in Krasnoyarsk among young people. To solve the problem, an online questionnaire was conducted among the residents of the city in order to clarify their attitude to martial arts. Based on the analysis of the data obtained, recommendations were proposed to attract the population and popularize freestyle wrestling classe

The Role of Transcription Factor PPAR-γ in the Pathogenesis of Psoriasis, Skin Cells, and Immune Cells

The peroxisome proliferator-activated receptor PPAR-γ is one of three PPAR nuclear receptors that act as ligand-activated transcription factors. In immune cells, the skin, and other organs, PPAR-γ regulates lipid, glucose, and amino acid metabolism. The receptor translates nutritional, pharmacological, and metabolic stimuli into the changes in gene expression. The activation of PPAR-γ promotes cell differentiation, reduces the proliferation rate, and modulates the immune response. In the skin, PPARs also contribute to the functioning of the skin barrier. Since we know that the route from identification to the registration of drugs is long and expensive, PPAR-γ agonists already approved for other diseases may also represent a high interest for psoriasis. In this review, we discuss the role of PPAR-γ in the activation, differentiation, and proliferation of skin and immune cells affected by psoriasis and in contributing to the pathogenesis of the disease. We also evaluate whether the agonists of PPAR-γ may become one of the therapeutic options to suppress the inflammatory response in lesional psoriatic skin and decrease the influence of comorbidities associated with psoriasis

Purine bases at position 37 of tRNA stabilize codon–anticodon interaction in the ribosomal A site by stacking and Mg(2+)-dependent interactions

The anticodon loop of tRNA contains a number of conserved or semiconserved nucleotides. In most tRNAs, a highly modified purine is found at position 37 immediately 3′ to the anticodon. Here, we examined the role of the base at position 37 for tRNA(Phe) binding to the A site of Escherichia coli ribosomes. Affinities and rate constants of A-site binding of native yeast peptidyl-tRNA(Phe) with hypermodified G (wybutine), or of unmodified peptidyl-tRNA(Phe) transcripts with G, A, C, or U, at position 37 were measured. The data indicate that purines stabilize binding due to stronger stacking and additional interactions with the ribosome mediated by Mg(2+) ions. Paromomycin, an antibiotic that binds to 16S rRNA in the decoding center, greatly stabilized tRNAs in the A site and abolished the Mg(2+)-dependence of binding. Comparison of binding enthalpies and entropies suggests that hypermodification of the base at position 37 does not affect stacking in the codon–anticodon complex, but rather decreases the entropic penalty for A-site binding. Substitution of purines with pyrimidines at position 37 increases the rates of tRNA binding to and dissociation from the A site. The data suggest that initial binding of tRNA to the A site is followed by a rate-limiting rearrangement of the anticodon loop or the ribosome decoding center that is favored by purines at position 37 and involves stronger stacking, additional Mg(2+) binding, and interactions with 16S rRNA

Fusion of Lysostaphin to an Albumin Binding Domain Prolongs Its Half-Life and Bactericidal Activity in the Systemic Circulation

Antibacterial lysins are promising proteins that are active against both antibiotic-susceptible and antibiotic-resistant bacterial strains. However, a major limitation of antibacterial lysins is their fast elimination from systemic circulation. PEGylation increases the plasma half-life of lysins but renders them inactive. Here we report the construction of a fusion protein of lysostaphin, a potent anti-staphylococcal lysin, and an albumin-binding domain from streptococcal protein G. The resulting fusion protein was less active than the parent enzyme lysostaphin, but it still retained significant antibacterial activity even when bound to serum albumin. The terminal half-life of the fusion protein in rats was five-fold greater than that of lysostaphin (7.4 vs. 1.5 h), and the area under the curve increased more than 115 times. Most importantly, this increase in systemic circulation time compensated for the decrease in activity. The plasma from rats that received an injection of the fusion protein retained bactericidal activity for up to 7 h, while plasma from rats that received plain lysostaphin lacked any detectable activity after 4 h. To the best of our knowledge, this is the first report of an antibacterial lysin with both improved pharmacokinetic parameters and prolonged bactericidal activity in the systemic circulation

The Influence of Dimerization on the Pharmacokinetics and Activity of an Antibacterial Enzyme Lysostaphin

The increasing prevalence of antibiotic-resistant strains of pathogenic bacteria is a major healthcare problem. Antibacterial lysins are enzymes that cleave the peptidoglycan of the bacterial cell wall. These proteins hold potential as a supplement or an alternative to traditional antibiotics since they are active against antibiotic resistant strains. However, antibacterial lysins are rapidly eliminated from the systemic circulation, which limits their application. Dimerization of an anti-pneumococcal lysin Cpl-1 has been demonstrated to decrease the clearance rate of this protein in mice. In the present work, we constructed a dimer of an anti-staphylococcal lysin lysostaphin by fusing it with an anti-parallel α-helical dimerization domain. Lysostaphin dimer had a more favorable pharmacokinetic profile with increased terminal half-life and area under the curve (AUC) values compared to monomeric lysostaphin. However, the staphylolytic activity of dimerized lysostaphin was decreased. This decrease in activity was likely caused by the dimerization; since the catalytic efficacy of lysostaphin dimer towards pentaglycine peptide was unaltered. Our results demonstrate that, although dimerization is indeed beneficial for the pharmacokinetics of antibacterial lysins, this approach might not be suitable for all lysins, as it can negatively affect the lysin activity

Comparative Analysis of the Training Specialists Directions in Physical Culture and Sports Profiles in Educational Institutions of Higher Education of the Ministry of Sports of the Russian Federation and Federal Universities

Востребованность в специалистах для физкультурно-спортивной отрасли постоянно растет, так как расширение доступной и комфортной спортивной инфраструктуры в большинстве регионов РФ способствует росту желающих систематически заниматься физическими упражнениями для сохранения и укрепления своего здоровья, повышать уровень спортивного мастерства в спорте высших достижений. В РФ более ста образовательных организаций высшего образования (ООВО) готовят профильных специалистов для отрасли, но большинство имеют небольшие квоты бюджетных мест для поступающих. Ведущую роль здесь играют четырнадцать специализированных ООВО Минспорта РФ. Они являются кузницей по выпуску специалистов по направлению подготовки «Спорт» (49.03.04), профили которого охватывают практически все развиваемые в РФ виды спорта. Для подготовки специалистов по направлению «Физическая культура» (49.03.01) существенный вклад вносят девять федеральных университетов. К тому же в пяти из них проводится обучение по педагогическому образованию с двумя профилями подготовки (44.03.05), позволяющему получить комплекс знаний и навыков в двух областях: педагогика и другая смежная дисциплина, которое отсутствует в ООВО Минспорта РФ. Перспективы обеих групп ООВО возрастают с учреждением на их базе Центров спортивной подготовки студенческих сборных команд (ЦСПССК). В ООВО Минспорта РФ проект уже запущен с 1 января 2023 г. В федеральных университетах готовится запуск центров развития студенческого спорта на базе СФУ и УрФУ, что повысит возможности этих ООВО для открытия и расширения направления подготовки «Спорт» (49.03.04) в образовательном процессеThe demand for physical culture and sports industry specialists is constantly growing, as the expansion of affordable and comfortable sports infrastructure in most regions of the Russian Federation contributes to the growth of those who want to engage systematically in physical exercises in order to preserve and strengthen their health, to increase the level of sportsmanship in high- performance sports. More than a hundred institutions of higher education train specialists for the industry in the Russian Federation, but most of them have small quotas of budget places for applicants. The leading role here is played by fourteen specialized institutions of higher education of the Ministry of Sports of the Russian Federation. They are producers of specialists in the direction of training sport: 49.03.04, which profiles professionals cover almost all sports developing in the Russian Federation. Nine federal universities make a significant contribution to the training of specialists in the direction of physical culture: 49.03.01. In addition, five of them provide training in pedagogical education with two training profiles (44.03.05), which allows you to obtain knowledge and skills in two areas: pedagogy and another related discipline, which is not available at the institutions of higher education in the Ministry of Sports of the Russian Federation. The prospects of both groups of institutions of higher education are increasing with the establishment of Sports Training Centers for Student National Teams on their basis. The project has already been launched since January 1, 2023 at the institutions of higher education of the Ministry of Sports of the Russian Federation. Federal universities are getting ready to launch student sports development centers at the base of Siberian and Ural Federal Universities, which will increase the capabilities of these universities to open and expand the direction of sports training (49.03.04) in the educational proces