Recovery of adiponectin from five different dried blood spot concentrations.

<p>Recovery of adiponectin from five different dried blood spot concentrations.</p

Typical calibration curve.

<p>Whole bloodspot adiponectin standards at 3.75, 7.5, 15.0, 30.46 µg/ml.</p

Adiponectin levels in boys and girls by demographic and clinical characteristics, N = 13,329.

<p>SD = standard deviation; CI = confidence interval.</p>a<p>Linear regression coefficient and 95% CI (age-adjusted, accounting for clustering by polyclinic).</p>b<p>Normal weight, overweight and obese as defined by the International Obesity Task Force BMI cut-offs. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071315#pone.0071315-Cole1" target="_blank">[44]</a>.</p>c<p>Centiles derived from NHANES European age and sex-specific thresholds (reference) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071315#pone.0071315-Fernandez1" target="_blank">[45]</a>.</p>d<p>Centile derived from sample.</p>e<p>Quartiles derived from NHSR age-and sex-specific thresholds <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071315#pone.0071315-McDowell1" target="_blank">[46]</a>.</p>f<p>Quartiles derived from sample.</p

Comparison of adiponectin levels measured in PROBIT with other studies in children of a similar age.

<p>SD = standard deviation; m = months;</p>a<p>median (interquartile range);</p>b<p>interquartile range;</p>c<p>95% CI.</p

Comparison of adiponectin concentrations measured from 50 paired plasma and whole bloodspot samples.

<p>Comparison of adiponectin concentrations measured from 50 paired plasma and whole bloodspot samples.</p

Stability of adiponectin concentrations (at A. low, B. medium and C. high values) measured from dried blood spots and stored at −80°C for 30 months.

<p>Adiponectin concentrations of internal quality control (IQC) bloodspots (low, medium and high values) analysed at regular intervals over 30 months. For the ‘low’ IQC sample (Fig. 2A), the middle red line is the mean adiponectin concentration and the upper and lower red lines are the 95% reference range (calculated from the standard deviation of 24 replicates); the respective lines showing the mean and 95% reference range for the ‘medium’ (Fig. 2B) and ‘high’ (Fig. 2C) IQC samples are also shown.</p

Bland-Altman plot for insulin values from fresh minus frozen samples.

<p>The difference in fasted bloodspot insulin from fresh sample minus the same sample frozen for 8 weeks at −80°C.</p

Stability of insulin concentrations measured from dried blood spots at +30°C for 1 weeks.

<p>Bloodspot fasting insulin concentrations from three samples analysed at baseline (time = 0), and after 1 hour, 12 hours, 24 hours, 48 hours and 1 week at +30°C.</p

Association between Teacher Assessed Strengths and Difficulties Questionnnaire (SDQ) and ChEAT scores ≥85^th percentile.

†<p>ORs adjusted for age, sex and cluster (polyclinic site). ^‡ORs adjusted for age, sex, cluster (polyclinic site), treatment arm, child's BMI at age 6.5 years and number of older children in household * (n = x, y, z): x =  total number of children in group, y =  total number of females in group, z =  total number of males in group.</p>§<p>Teacher SDQ associations also adjusted for teacher ID as a cluster variable.</p><p>Teacher SDQ measures have been categorized as “normal”, “borderline” and “abnormal”, according to standardized cut-off points for the SDQ, for the presentation of results, although SDQ score was included as a continuous, standardized variable in mixed-effects logistic regression models.</p

Stability of fasting insulin stored at −80°C for 26 months.

<p>Fasting insulin concentrations of three bloodspot samples analysed prior to freezing (time 0) and then at regular intervals over 26 months. For sample 1, the middle red line is the insulin concentration at time 0 and the upper and lower red lines are the 95% reference range (calculated from the standard deviation of 30 replicates of the time 0 value). For samples 2 and 3, the lines are coloured green and blue, respectively.</p