Viral selectivity measured in percentage of reads.

<p>(A) Viral selectivity for the tested concentration methods (B) and extraction methods. Each boxplot was made from 12 individual samples (including the four extraction/concentration methods with three replicates each). The bar, box, whiskers and circles represents median, inter-quartile range, inter-quartile range times 1.5, and outliers, respectively. Asterisks represent significance level of a pairwise t-test with “Holm-Bonferroni” adjusted p-values. ** = p < 0.01, *** = p < 0.001.</p

The influence of extraction method on the viral community composition.

<p>PCA plots made by using the relative abundances of the nine most abundant viral families. Separate PCAs were done for (A) samples concentrated with MAF, (B) SMF, (C) GW, and (D) PEG. Sample replicates were individually plotted and grouped according to the extraction method. In cases where only two samples were present, no ellipse representing the cluster was drawn.</p

Detection of pathogenic viral families.

<p>Heatmap of the relative abundance of 14 human pathogenic viral families, detected by the 16 different concentration/extraction combinations. The numbers within each cell represents reads per million. The colours range from green = no detection, to red = high relative abundance.</p

Viral species richness.

<p>(A) Viral Chao 1 species richness of the tested concentration methods, and (B) extraction methods. Each boxplot was made from 12 individual samples (including the four extraction/concentration methods with three replicates each). The bar, box, whiskers and circles represents median, inter-quartile range, inter-quartile range times 1.5, and outliers, respectively. Asterisks represent significance level of a pairwise t-test with “Holm-Bonferroni” adjusted p-values. * = p < 0.05, ** = p < 0.01, *** = p < 0.001.</p

The influence of concentration method on the viral community composition.

<p>PCA plots made by using the relative abundances of the nine most abundant viral families. Separate PCAs were done for (A) samples extracted with QIA, (B) POW, (C) MIN, and (D) NUC. Sample replicates were individually plotted and grouped according to the concentration method. In cases where only two samples were present, no ellipse representing the cluster was drawn.</p