بررسی ارتباط فاکتور CA19_9 با کنسرهای گوارشی و مرحله آنها در شهر قزوین در سال ۹۴_۹۸

عنوان مطالعه : بررسی ارتباط فاکتور CA19_9 با کنسرهای گوارشی و مرحله آنها در شهر قزوین در سال ۹۴_۹۸ زمینه و هدف : با توجه به شیوع بالای کنسر های گوارشی بخصوص کنسر پانکراس در این طرح با بررسی ارتباط فاکتور CA19_9 با فاکتور های تعیین کننده یک کنسر میتوان از نتایج آن در پروگنوز بهتر کنسر ها استفاده کرد تومور مارکر ها همواره یکی از روش های تشخیصی سرطان ها در کنار روش های رادیولوژیک بوده اند. تومور مارکر CA19_9 یکی از تومور مارکر های مورد استفاده بخصوص در کنسر پانکراس میباشد. در این طرح به بررسی قدرت تشخیصی این تومور مارکر در انواع سرطان های گوارشی پرداخته میشود مواد و روش ها : در این طرح ابتدا با استفاده از مستندات پاتولوژی موجود در پرونده بیمارانی که در بیمارستان ولایت شهر قزوین مورد آزمایش قرار گرفته بودند، مواردی که در آنها فاکتور CA19_9 افزایش یافته دارند، استخراج میشود. سپس با استفاده از اطلاعات بیماران موجود در بررسی پاتولوژی انجام گرفته، بیماران را از نظر وجود یا عدم وجود کنسر گوارشی بررسی کرده. یافته ها: از نتایج حاصل شده مشخص شد که حساسیت CA19_9 در تشخیص سرطان های گوارشی 23% و ویژگی آن 71% میباشد. همپنین مشخص شد که بین مارکر CA19_9 با سن (P= 0.277 ) و جنسیت (P=0.215) و با لنف نود درگیر (P=0.561 ) و با سایز تومور (P=0.172) و با مرحله سرطان (P=0.297 ) ارتباط معناداری وجود نداشت. نتیجه گیری کلی: طبق نتایج این مطالعه تومور مارکر CA19_9 حساسیت بالایی در تشخیص سرطان های گوارشی ندارد و در موارد خوش خیم گوارشی مثل انواع گاستریت ها نیز افزایش میابد. کلید واژه ها: سرطان گوارشی – CA19-9 –_سرطان پانکراس _ سرطان کولورکتال_ تومور مارک

The Association of Methylation Status and Expression Level of MyoD1 with DNMT1 Expression Level in Breast Cancer Patients

Background: Breast cancer (BC) is the most common malignancy in women worldwide. The methylation status of MyoD1, a tumor suppressor gene, is enrolled in various cancers, i.e., BC. Various studies showed the impact of MyoD1 epigenetic dysregulation in BC. This study aimed to investigate the methylation status and expression level of MyoD1 in BC patients and its association with the expression of DNMT1. Materials and Methods: This case-control study was conducted on 30 cases (pathology-confirmed ductal carcinoma) and 18 controls (fibroadenoma and fibrocystic masses), referred to Velayat Hospital, Qazvin, Iran. The expression of the MyoD1 and DNMT1 and the promoter methylation of the MyoD1 were evaluated in tissue blocks of BC patient masses using qRT-PCR and MS-PCR assays, respectively. SPSS 24.0 was used to analyze the data. Results: The MyoD1 promoter is hypermethylated in BC patients compared to controls (p =0.001). The expression level of MyoD1 in BC patients was significantly reduced compared to controls (fold change =0.13, p =0.042). In addition, in BC patients, the reduced expression level of MyoD1 was significantly associated with methylation of the MyoD1 promoter (p =0.001). There is no significant difference between the expression level of DNMT1 in BC patients and controls (p =0.197). A significant association is found between the expression of DNMT1 and the methylation status of the MyoD1 promoter (p =0.038). Discussion: The expression level of MyoD1 is affected by the methylation status of the promoter of this gene. Moreover, the expression level and methylation status of MyoD1 are correlated with clinical parameters

Detection of bacterial agents causing prostate infection by culture and molecular methods from biopsy specimens

Background and Objectives: Prostatitis affects about 16% of men in their lifetime and sometimes leading to prostate cancer. Bacterial infections are the most common causes of prostatitis. Diagnosis of the causative agents of bacterial prostate infections plays an essential role in timely treating and preventing secondary complications. This study isolated bacterial infectious agents in patients’ surgical prostate and evaluated them by routine and molecular microbiological methods. Materials and Methods: In this cross-sectional study, 72 prostate biopsy specimens were collected from the Orology Departmen of hospitals of Qazvin University of Medical Sciences. All samples were cultured in aerobic and anaerobic conditions. Antibiotic susceptibility test by Kirby-Bauer standard method was performed for all isolated bacteria. In addition, all isolated bacteria were identified using 16S rDNA PCR and sanger sequencing methods. Also, TaqMan real-time PCR was applied to detect Ureaplasm aurealyticum, Mycoplasma hominins, and Mycoplasma genitalium. Results: In conventional culture method, out of 18 positive samples, 15 samples (83.3%) were Gram-negative bacteria and 3 samples (16.6%) were Gram-positive bacteria, containing Escherichia coli (55.5%), Klebsiella pneumoniae (11.1%), Enterobacter cloacae (5.5%), Pseudomonas aeruginosa (11.1%), Staphylococcus aureus (11.1%), and Enterococcus faecalis (5.5%). The results of molecular identification methods were the same as conventional culture results. Also, four patients were Ureaplasm aurealyticum, and three patients were positive for Mycoplasma hominis. Conclusion: Most bacteria isolated from prostate specimens belonged to the Enterobacteriaceae family, especially Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae. Staphylococcus aureus and Enterococcus faecalis were cocci isolated in the specimens too. Also, Ureaplasma urealyticum, and Mycoplasma hominis were identified in prostatitis. Keywords: Prostatitis; Pathogens; Enterobacteriaceae; 16s rDNA; Real-time polymerase chain reactio

The Relationship Between Gastric Cancer and Helicobacter Pylori in Formaldehyde Fixed Paraffin Embedded Gastric Tissues of Gastric Cancer Patients-Scorpion Real-Time PCR Assay Findings

Abstract Gastric cancer is the second leading cause of cancerrelated deaths worldwide and it seems that environmental and lifestyle factors and infection with Helicobacter pylori (H.pylori) have had a major role in the etiology of gastric cancer. The aim of this study was to investigate the presence of H. pylori DNA in archival gastric tissues of patients with gastric cancer disease by rapid, sensitive and specific technique of Scorpion Realtime PCR. This retrospective cross-sectional study was performed on 285 paraffin embedded gastric specimens of patients who were pathologically proved for gastric cancer admitted in Bou- Ali, Shahid Rajaie and Dehkhoda hospitals and Bahar and Farzam private laboratory in Qazvin city in Iran during 2009 and 150 paraffin embedded pathological specimens of patients with other proved diagnosis other than gastric cancer. Results of our Scorpion Realtime PCR analysis showed that DNA of H. pylori DNA was present in 78.42 % of our total specimens. Modified McMullen’s Staining of paraffin embedded sections was positive in 210 patients. Also we were not able to finding significant relationship between demographic characteristics of our studied patients and presence of H. pylori DNA in their formaldehyde fixed paraffin embedded gastric tissues samples. Existence of H. pylori in gastric tissue samples of patients with gastric cancer is controversial and our results indicated that in our studied specimens prevalence of H.pylori was significantlymore than recent published reports

Development of a SYBR Green Multiplex Real Time PCR for Simultaneous Detection of Mycobacterium Tuberculosis and Nocardia Asteroides in Respiratory Samples

BACKGROUND፡ Nocardia asteroides and Mycobacterium tuberculosis are worldwide-distributed bacteria. These infectious agents can cause many infections in humans, especially in immunocompromised individuals. Pulmonary infections are more common and have similar clinical symptoms. Proper diagnosis and treatment of these patients are important for accurate treatment and could be lifesaving. METHODS: In this study, a multiplex real-time PCR assay was established for the simultaneous detection of the N. asteroides and M. tuberculosis. Both this homemade multiplex real time PCR and routine commercial tuberculosis tests were performed on 150 pulmonary specimens collected from individuals suspected to have tuberculosis. RESULTS: From 150 specimens, 20 samples were acid fast positive, 14 positives for M. tuberculosis by singleplex real time PCR, 10 positives for N. asteroides by singleplex real time PCR and 2 positives for M. tuberculosis and N. asteroides by multiplex real time PCR whereas 14 samples were positive for M. tuberculosis with commercial test. Differential diagnosis of pulmonary tuberculosis is useful for their proper treatment. CONCLUSION: Our test had good performance for differential diagnosis of tuberculosis and nocardiosis. Therefore, it is recommended to be used to diagnose such patients. KEYWORDS: Mycobacterium tuberculosis, Nocardia asteroides, Respiratory sample