Convenio de cooperación técnica: Reutilización agrícola forestal de aguas tratadas en Ingeniero Jacobacci

El uso de aguas residuales tratadas en riego agrícola forestal es una alternativa para evitar el vuelco a cuerpos receptores hídricos, que resulta de especial interés en áreas donde la escasez de agua afecta las actividades humanas. La zona centro de la provincia de Río Negro integra los dos tercios de la superficie en la Argentina que presenta balances hídricos negativos la mayor parte del año, esto representa una importante limitación para todo tipo de actividades productivas y en muchos casos hasta se dificulta el abastecimiento de agua para consumo humano.Estación Experimental Agropecuaria BarilocheFil: Cremona, Maria Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Recursos Naturales; ArgentinaFil: Velasco, Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Agencia de Extensión Rural Ingeniero Jacobacci; ArgentinaFil: Zuñiga, Aldo Mario. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Recursos Naturales; ArgentinaFil: Tanzer, Laura. Departamento Provincial de Aguas. Rio Negro; ArgentinaFil: Magnin, Santiago. Departamento Provincial de Aguas. Rio Negro; ArgentinaFil: Fernandez, Patricia. Departamento Provincial de Aguas. Rio Negro; ArgentinaFil: Alemanni, Maria Eugenia. Departamento Provincial de Aguas. Rio Negro; ArgentinaFil: Riat, Martha. Universidad Nacional de Rio Negro; ArgentinaFil: Laos, Francisca. Universidad Nacional de Rio Negro; ArgentinaFil: Capuano, Ana. Universidad Nacional de Rio Negro; ArgentinaFil: Güevara, Tomas. Universidad Nacional de Rio Negro; ArgentinaFil: Fornasa, Alejandro. Cooperativa de agua y otros servicios públicos de Ingeniero Jacobacci; ArgentinaFil: Mellado, José. Cooperativa de agua y otros servicios públicos de Ingeniero Jacobacci; ArgentinaFil: López, Oscar. Cooperativa de agua y otros servicios públicos de Ingeniero Jacobacci; ArgentinaFil: Currumán, Antonio. Municipalidad de Ingeniero Jacobacci; ArgentinaFil: Nasif, Abdel. Ente para el Desarrollo de la Línea y región Sur; Argentin

Modified Adenovirus Reduces De Novo Peritoneal Adhesions in Rats and Limits Off-Target Transfection. Role of EZH2 in Adhesion Formation

Aim of the study: Adenovector encoding tissue plasminogen activator (tPA) was shown to reduce experimental peritoneal adhesion. We investigated the targeting potential of our modified adenovector, its ability to reduce adhesions and the epigenetic role of histone methyltransferase EZH2 in adhesion formation. Materials and methods: Control lacZ, nonmodified tPA or modified tPA vectors were instilled in the peritoneal cavity after injury in de novo adhesions or after lysis of adhesions in recurrent adhesions. Adhesion severity was scored and adhesions and liver tissues were examined for adenovirus E4 gene and tPA mRNA expression. Levels of tPA, plasminogen activator inhibitor-1 (PAI-1), transforming growth factor-β1 (TGF-β1), and EZH2 expression were measured. Results: E4 transcripts were detected in adhesions of nonmodified and modified and in livers of nonmodified but not in livers of modified de novo adhesions. Both nonmodified (p = 0.021) and modified vectors (p = 0.036) reduced the severity of de novo adhesions compared to lacZ vector. Levels of tPA in nonmodified (p = 0.021) and modified adhesions (p = 0.001) were elevated while PAI-1 (p = 0.013 and p = 0.001, respectively) and TGF-β1 levels (p = 0.002 and p = 0.016, respectively) were reduced compared with lacZ group. All vectors were not expressed in recurrent adhesions and severity score were not different among groups. EZH2 levels were elevated in de novo nontreated (p = 0.001) and was further increased in recurrent (p = 0.001) nontreated adhesions compared with noninjured peritoneum. Conclusion: Modified adenovirus successfully targeted de novo adhesions but not liver tissues and reduced the severity of de novo adhesions. EZH2 is involved in the development and progression of peritoneal adhesions

Diagnostic value of fibronectin discriminant score for predicting liver fibrosis stages in chronic hepatitis C virus patients

Background. Several noninvasive predictive models were developed to substitute liver biopsy for fibrosis assessment.Aim. To evaluate the diagnostic value of fibronectin which reflect extracellular matrix metabolism and standard liver functions tests which reflect alterations in hepatic functions.Material and methods. Chronic hepatitis C (CHC) patients (n = 145) were evaluated using ROC curves and stepwise multivariate discriminant analysis (MDA) and was validated in 180 additional patients. Liver biochemical profile including transaminases, bilirubin, alkaline phosphatase, albumin, complete blood count were estimated. Fibronectin concentration was determined using monoclonal antibody and ELISA.Results. A novel index named fibronectin discriminant score (FDS) based on fibronectin, APRI and albumin was developed. FDS produced areas under ROC curves (AUC) of 0.91 for significant fibrosis and 0.81 for advanced fibrosis. The FDS correctly classified 79% of the significant liver fibrosis patients (F2–F4) with 87% sensitivity and 75% specificity. The relative risk [odds ratio (OR)] of having significant liver fibrosis using the cut-off values determined by ROC curve analyses were 6.1 for fibronectin, 4.9 for APRI, and 4.2 for albumin. FDS predicted liver fibrosis with an OR of 16.8 for significant fibrosis and 8.6 for advanced fibrosis. The FDS had similar AUC and OR in the validation group to the estimation group without statistically significant difference. Conclusion. FDS predicted liver fibrosis with high degree of accuracy, potentially decreasing the number of liver biopsy required