Effect of using different levels of pistachio by-products silage on composition and blood parameters of Holstein dairy cows

The goal of this research was to study about the effect of using pistachio hulls silage in cow's nutrition. The initial experiment was about the effect of several feed additives on chemical and fermentative characteristic of pistachio hulls silages. This experiment was designed with 5 treatments and 4 replications in each treatment in a complete randomized design. Additives for preparing pistachio hulls silage were in the sequence of 1.5% citrus pulp (in base of dry matter), 1.5% ground barley, 1.5% beet pulp, 1.5% molasses and one control treatment without any additives. Silages were sampled for chemical analysis and fermentative characteristics. Results of the analysis showed no significant variation between the treatments in respect of ash. In the second experiment, the application of pistachio hulls silage in the nutrition of lactating cows was studied. Eight lactating cows in one Latin square design experiment were used. Treatments included various amounts of pistachio hulls silage replaced with corn silage (in base of dry mater). The results show no significant variation between the treatments in milk, blood, rumen factors and digestibility factors that can show pistachio hulls silage that can be replaced with corn silage up to 15% (%dry matter) in dairy cows ration without any significant variation in cows production.Key words: Dairy cattle's, milk production, pistachio by-product, silage, tannin

Potential nutritive value of some forage species used as ruminants feed in Iran

A study was conducted to determine rumen degradability (in sacco) of dry matter and in vitro gas production of the most important forage species grown in Iran, to compare forage species according to calculated degradation and in vitro gas production parameters, and to establish prediction equations for relative feed value (RFV) from gas production parameters. Thus, six forage species consisting of Lucerne, Eruca sativa, Crocus sativus, Cardaria draba, Setaria Spp., and Triticum aestivum forages were evaluated. Crude protein (CP) contents in the forages ranged from 139.60 to 246.30 g kg-1 DM. The neutral detergent fiber (NDF), acid detergent fiber (ADF) and organic matter (OM) were 273.30 to 596.60, 210.00 to 310.00, and 820.00 to 946.70 g kg-1 DM, respectively. The highest DM degradation and in vitro gas producton parameters were found for E. sativa. Also the relative feed value (RFV), relative forage quality (RFQ), dry mater intake and effective dry matter digestibility calculated for E. sativa was significantly (P < 0.05) higher than other forages. The variation of RFV explained by the gas production parameters ranged (R2) from 0.023 to 0.846. The gas production at 6, 24 and 48 h incubation times explained 0.836, 0.800 and 0.805 of variation of RFV, respectively. There was a negative correlation between in vitro gas production in different time incubation with NDF, ADF and a positive correlation between gas production parameters and CP content of forage species. The study shows that these forages relatively had a good nutritive value in comparison of Lucerne, and therefore, may serve as potential supplements for ruminants in Iran, and it seems that RFV index of six forage species such as used in this present study may be predicted from in vitro gas production parameters.Keywords: Forage species, in vitro gas production, in situ degradability, relative feed valu

The effects of addition of omega-3, 6, 9 fatty acids on the quality of bovine chilled and frozen-thawed sperm

This study was aimed to investigate the effects of omega-3, 6, 9 fatty acids on the characteristics of bovine chilled and frozen-thawed semen. For this purpose, oil containing different levels of omega-3, 6, 9 fatty acids were added to semen extender. To emulsify the oil in semen extender, polyethylene glycol (PEG) was added as a suitable solvent and the solution was finally sonicated. Five proven Holstein bulls were randomly selected and their ejaculates were collected using an artificial vagina. Groups were designed as control, treatments 1, 2, 3 and 4. The control group contained only the basic extender (Tris-citrate buffer, egg yolk and glycerol) without any additives. In treatment 1, only 5% PEG was added to the diluent; while in treatments 2, 3 and 4 different concentrations of omega-3, 6, 9 fatty acids (1.0, 2.5 and 5.0%) in combination with PEG were added to the basic extender. After dilution, the semen samples were packaged into 0.5 ml straws, a process that was followed by cooling the semen straws. Motility, viability and morphology of semen samples were evaluated after 24 and 48 h of storage in refrigerator (5 ˚C) or after one month of storage in the liquid nitrogen. Immotility was increased and all the other parameters including motility, viability and morphology were significantly decreased in all the groups compared with fresh samples during cold storage and freezing-thawing. Our results demonstrated the following: 1) PEG has significant detrimental effects, especially on the sperm motility; 2) addition of omega-3, 6, 9 fatty acids could not improve the sperm motility in chilled storage condition and after freezing-thawing; and 3) omega-3, 6, 9 fatty acidscould not also attenuate the other deleterious effects of PEG. In conclusion, our findings reveal that addition of these fatty acids to the semen extender does not enhance the resistance of the bovine sperm membrane to cooling and freezing-thawing and that further studies are required to find suitable candidate compounds that can boost the quality of semen that is chilled and freeze-thawed.Keywords: Bull, Cyropreservation, Omega-3, 6, 9 fatty acids, Semen qualit

Potential use of high-temperature and low-temperature steam treatment, sodium hydroxide and an enzyme mixture for improving the nutritional value of sugarcane pith

The effectiveness of different treatment methods to improve the nutritional value of the sugarcane by-products (pith or bagasse) has been evaluated. The treatment methods included a high-pressure steam treatment (HPST; 19 bar, 3 min), treating the products with sodium hydroxide, sulphuric acid plus an enzyme mixture, or low-temperature steam treatment (LTST) under different conditions. Gas production (GP), two-step in vitro digestibility (IVD) and in situ degradability (ISD) techniques were used to monitor the effectiveness of the treatments. HPST resulted in a significant increased in the total soluble sugar (TSS) content of unsteamed pith (USP), 20 vs. 123.75 mg/100 mL. Except for the enzyme treatment, the other treatments led to a significant improvement in the nutritional value of sugarcane by-products, as measured by the IVD method. LTST resulted in an increase in potential GP (B) at higher temperature, reaction time and amount of acid. The highest potential GP (110.92 mL/300 mg DM) was achieved under the conditions, 134 °C, 18 g acid/kg DM, 120 min, and the lowest (72.4 mL/300 mg DM) under the conditions, 121 °C, no acid, 40 min. In situ dry matter degradability (ISDMD) was unaffected by LTST. Dry matter digestibility results indicated that the optimal treatments for treating pith were HPST and NaOH, but that enzymes were ineffective. Furthermore, considering treatment cost (creating high-pressure are more expensive than low temperature treatments), potential environmental health problems and the relative improvement in the nutritional value of pith achieved by the LTST + acid method, compared to the HPST method (as measured using GP), these results suggested that the methods based on the use of LTST and acid (especially under harsher conditions), have the best potential to improve the nutritive value of sugarcane by-products.Keywords: Saccharum officinarum, steam, bagasse, raw or unsteam-treated pit