Inhibition of vascular smooth muscle growth via signaling crosstalk between AMP-activated protein kinase and cAMP-dependent protein kinase

Abnormal vascular smooth muscle (VSM) growth is central in the pathophysiology of vascular disease yet fully effective therapies to curb this growth are lacking. Recent findings from our lab and others support growth control of VSM by adenosine monophosphate (AMP)-based approaches including the metabolic sensor AMP-activated protein kinase (AMPK) and cAMP-dependent protein kinase (PKA). Molecular crosstalk between AMPK and PKA has been previously suggested, yet the extent to which this occurs and its biological significance in VSM remain unclear. Considering their common AMP backbone and similar signaling characteristics, we hypothesized that crosstalk exists between AMPK and PKA in the regulation of VSM growth. Using rat primary VSM cells (VSMC), the AMPK agonist AICAR increased AMPK activity and phosphorylation of the catalytic Thr172 site on AMPK. Interestingly, AICAR also phosphorylated a suspected PKA-inhibitory Ser485 site on AMPK, and these cumulative events were reversed by the PKA inhibitor PKI suggesting possible PKA-mediated regulation of AMPK. AICAR also increased PKA activity in a reversible fashion. The cAMP stimulator forskolin increased PKA activity and completely ameliorated Ser/Thr protein phosphatase-2C activity, suggesting a potential mechanism of AMPK modulation by PKA since inhibition of PKA by PKI reduced AMPK activity. Functionally, AMPK inhibited serum-stimulated cell cycle progression and cellular proliferation; however, PKA failed to do so. Moreover, AMPK and PKA reduced PDGF-β-stimulated VSMC migration. Collectively, these results show that AMPK is capable of reducing VSM growth in both anti-proliferative and anti-migratory fashion. Furthermore, these data suggest that AMPK may be modulated by PKA and that positive feedback may exist between these two systems. These findings reveal a discrete nexus between AMPK and PKA in VSM and provide basis for metabolically-directed targets in reducing pathologic VSM growth

Assessing Rural and Urban Community Assets and Needs to Inform Extension Program Planning

A needs assessment is a useful tool for prioritizing community needs and allocating resources. Prioritizing community needs helps ensure Extension programs are relevant and targeted towards specific audiences. This study prioritized normative needs of urban and rural Utah residents using a needs assessment framework. Convenience data were gathered from 1,043 adult Utah residents, and the raking method was used to weigh the sample by selected population characteristics. Descriptive statistics (frequencies and nonparametric statistics) were used for data analysis. A calculated Point-Score represented the difference between residents’ perceived importance and satisfaction of various community assets. Results showed affordable housing, affordable medical clinics, well-paying jobs, quality public schools, and affordable internet were the top five needs in urban areas. Utah rural residents ranked well-paying jobs, quality public schools, steady jobs, emergency healthcare, and affordable housing as high priority needs in their communities. Utah State University Extension should tailor existing programs to address urban and rural community needs, allocate resources to create new programs aligned to those needs, facilitate collaborations with local organizations, and conduct timely needs assessments to monitor changing community needs

Application of a Three-Phase Needs Assessment Framework to Identify Priority Issue Areas for Extension Programming

Cooperative Extension strives to deliver relevant programming to residents. However, problems facing communities are increasingly complex. We used a three-phase needs assessment to describe Utah residents\u27 perceptions of issue areas for Extension programming. We gathered data from 1,043 Utah residents. Results highlighted four priority issue areas for Extension programming. These were environmental quality, conservation capacity, community development, and agriculture and food safety. On the basis of our work, we recommend that Extension allocate resources to addressing priority issue areas and implement a formal needs assessment framework for monitoring issue areas to inform relevant and quality programming in response to emerging needs

A Descriptive Analysis of Remote Work Adoption in the United States

Advances in information and communications technology have enabled organizations to shift traditional work functions away from place or where work is accomplished to how work is accomplished (i.e., task facilitation). With the rise in remote work, there was a need to describe the adoption process by organizations in the United States. Given that the practice of remote work is considered an innovation, this quantitative study was guided by the theory of Diffusion of Innovations and followed a nonexperimental design with a correlational analysis, collecting cross-sectional data from a sample of organizational leaders in the United States (N = 1,259). Results describe where organizations range in the innovation-decision process of remote work adoption and categorize organizations based on innovativeness. This research demonstrates the role of COVID-19 in precipitating organizations’ rapid implementation of remote work during a pandemic. Findings hold implications for leaders deciding whether to adopt remote work as a formal workplace practice and can assist them in making informed operational decisions. Findings also provide Extension professionals with insights into responding to the social and economic consequences of the widespread adoption of remote work with relevant, research-based educational programming in their local communities

Kinetic equation approach to graphene in strong external fields

The report presents the results of using the nonperturbative kinetic approach to describe the excitation of plasma oscillations in a graphene monolayer. As examples the constant electric field as well as an electric field of short high-frequency pulses are considered. The dependence of the induced conduction and polarization currents characteristics on the pulse intensity, pulse duration, and polarization is investigated. The characteristics of secondary electromagnetic radiation resulting from the alternating currents is investigated. The nonlinear response to the external electric field characterizes graphene as an active medium. Qualitative agreement is obtained with the existing experimental result of measurements of currents in constant electric fields and radiation from graphene in the case of excitation by means of the infrared and optical pulses.Comment: 12 pages, 14 figures, 1 table, version published in Particle

Action Anthropology and Pedagogy: University-Community Collaborations in Setting Policy

This article describes a student-led, community-participatory project focused on reducing the burden of childhood lead poisoning in rental housing. A multidisciplinary group of students and faculty worked with community members. We compiled the social, public health, economic, and policy information on the human and fiscal costs of childhood lead poisoning. This analysis was done for community advocates to use to persuade policymakers to enact a local law strengthening the prevention of childhood lead poisoning in rental property. In conducting this work, the students gained experience in qualitative research methods, quantitative data analysis, the health consequences of lead exposure, health policy, urban health, science writing, and public presentation

Evaluating Utah\u27s Rural Online Initiative: Empowering Organizational Leaders Through Remote Work

Compared to urban counties, Utah\u27s rural counties experienced high levels of unemployment. Informed by a statewide needs assessment, Utah State University Extension developed a remote work leadership course to equip business leaders with knowledge and skills to create remote jobs as a solution to rural unemployment. This descriptive evaluation study collected data from course participants (N = 62). Findings showed short-term outcomes were achieved; participants experienced increases in knowledge and skills and had more positive intentions toward creating remote jobs and hiring employees from rural counties. Extension professionals can design and evaluate their programs using the framework in this study

Nonperturbative Kinetic Description of Electron-Hole Excitations in Graphene in a Time Dependent Electric Field of Arbitrary Polarization

On the basis of the well-known kinetic description of e − e + vacuum pair creation in strong electromagnetic fields in D = 3 + 1 QED we construct a nonperturbative kinetic approach to electron-hole excitations in graphene under the action of strong, time-dependent electric fields. We start from the simplest model of low-energy excitations around the Dirac points in the Brillouin zone. The corresponding kinetic equations are analyzed by nonperturbative analytical and numerical methods that allow to avoid difficulties characteristic for the perturbation theory. We consider different models for external fields acting in both, one and two dimensions. In the latter case we discuss the nonlinear interaction of the orthogonal currents in graphene which plays the role of an active nonlinear medium. In particular, this allows to govern the current in one direction by means of the electric field acting in the orthogonal direction. Investigating the polarization current we detected the existence of high frequency damped oscillations in a constant external electric field. When the electric field is abruptly turned off residual inertial oscillations of the polarization current are obtained. Further nonlinear effects are discussed

Quality of reporting of trial abstracts needs to be improved: using the CONSORT for abstracts to assess the four leading Chinese medical journals of traditional Chinese medicine

<p>Abstract</p> <p>Background</p> <p>Due to language limitations, the abstract of journal article may be the only way for people of non-Chinese speaking countries to know about trials in traditional Chinese medicine (TCM). However, little is known about the reporting quality of these trial abstracts. Our study is to assess the reporting quality of abstracts of randomized controlled trials (RCT) published in four leading Chinese medical journals of TCM, and to identify any differences in reporting between the Chinese and English version of the same abstract publication.</p> <p>Method</p> <p>Two reviewers hand-searched the Chinese Journal of Integrated Traditional and Western Medicine, the Chinese Journal of Integrative Medicine, the China Journal of Chinese Materia Medica and the Chinese Acupuncture & Moxibustion for all abstracts of RCTs published between 2006 and 2007. Two reviewers independently assessed the reporting quality of the Chinese and English version of all eligible abstracts based on a modified version of the CONSORT for reporting randomised trials in journal and conference abstracts (CONSORT for abstracts).</p> <p>Results</p> <p>We identified a total of 345 RCTs of TCM with both a Chinese and English abstract. More than half of Chinese abstracts reported details of the trial participants (68%; 234/345), control group intervention (52%; 179/345), the number of participants randomized (73%; 253/345) and benefits when interpreting the trial results (55%; 190/345). Reporting of methodological quality or key features of trial design and trial results were poor; only 2% (7/345) included details of the trial design, 3% (11/345) defined the primary outcome, 5% (17/345) described the methods of random sequence generation, and only 4% (13/345) reported the number of participants analyzed. No abstracts provided details on allocation concealment and trial registration. The percentage agreement in reporting (between the Chinese and English version of the same abstract) ranged from 84% to 100% across individual checklist item.</p> <p>Conclusion</p> <p>The reporting quality of abstracts of RCTs published in these four TCM journals needs to be improved. Since none of the four journals adopted CONSORT for Abstracts, we hope that the introduction and adoption of CONSORT for Abstracts by TCM journals will lead to an improvement in reporting quality.</p

Action Anthropology and Pedagogy: University-Community Collaborations in Setting Policy

This article describes a student-led, community-participatory project focused on reducing the burden of childhood lead poisoning in rental housing. A multidisciplinary group of students and faculty worked with community members. We compiled the social, public health, economic, and policy information on the human and fiscal costs of childhood lead poisoning. This analysis was done for community advocates to use to persuade policymakers to enact a local law strengthening the prevention of childhood lead poisoning in rental property. In conducting this work, the students gained experience in qualitative research methods, quantitative data analysis, the health consequences of lead exposure, health policy, urban health, science writing, and public presentation