1 research outputs found
Isolation and characterization of the tissue and development-specific potato snakin-1 promoter inducible by temperature and wounding
snakin-1 (StSN1) is a broad-spectrum antimicrobial peptide isolated
from Solanum tuberosum . Homologous proteins have been identified in a
wide range of species but there is no apparent consensus in the roles
they play. A 1394 bp fragment of the 5'upstream region of StSN1 gene,
designated PStSN1, was isolated from the potato genome and sequenced.
Bioinformatics analyses revealed a total of 55 potential regulatory
motifs related to tissue-specificity, stress, defence and hormone
responsiveness, among others. PStSN1 spatial and temporal activity was
studied in transgenic Arabidopsis plants expressing a reporter gene
under this promoter control (PStSN1::GUS). Histochemical staining
revealed PStSN1::GUS expression in the root vasculature, cotyledons,
young leaves and floral organs. Moreover, GUS staining was detected in
young developmental stages gradually decreasing as the plant aged.
Stress treatments on transgenic plants showed that PStSN1 activity was
induced by high/low temperature and wounding. The characterization of
PStSN1 in a model plant establishes a framework for the understanding
of its possible biological functions and provides a potential tool for
plant modification through genetic engineering