45 research outputs found
Bivariate statistics.
<p>(*β significant at 0.01 level of significance, ** β significant at 0.05 level of significance).</p><p>Legend: 1 β Gender, 2 β Age, 3 β Educational level, 4 β Marital status, 5 β Nature of industry, 6 β Current employment level, 7 β Income level, 8 β Frequency of volunteering, 9 β Duration of volunteering, 10 β Nature of contact, 11 β Self esteem, 12 β Employer encouragement, 13 β Job performance, 14 β Life satisfaction.</p
Final framework (based on significant coefficients).
<p>Final framework (based on significant coefficients).</p
Difference in <i>P. aeruginosa</i> cell surface.
<p>(A and B) Schematic showing changes in PA14 wildtype and Ξ<i>phzA-G</i> cell surface respectively before and after DNase I treatment. (A) Natural production of pyocyanin by wildtype strain facilitates EPS (mostly constitute of eDNA) binding to <i>P. aeruginosa</i> cell wall and removal of eDNA by DNase I treatment subsequently removes EPS from its cell wall. (B) The mutant Ξ<i>phzA-G</i> deficient in pyocyanin production could not facilitate eDNA/EPS binding with <i>P. aeruginosa</i> cell wall, therewith no effect of DNase I treatment on its cell wall.</p
Size analysis of <i>P. aeruginosa</i> PA14 strains.
<p>(A to C) Hydrodynamic diameter of PA14 wildtype and Ξ<i>phzA-G</i> strains, grown over 1 to 3 days respectively and effect of DNase I treatment on it. (D) Represents the change in reduction in hydrodynamic diameter of wildtype and Ξ<i>phzA-G</i> after DNase I treatment. Error bars represents standard deviations from the mean (nβ=β5). Asterisks and hash indicate statistically significant (p<0.05) differences in hydrodynamic diameter in comparison to DNase I treated wildtype and Ξ<i>phzA-G</i> strain (regardless of DNase I treatment) respectively. Dollar indicates statistically significant (p<0.05) differences in reduction in hydrodynamic diameter between wildtype (before and after DNase I treatment) and Ξ<i>phzA-G</i> (before and after DNase I treatment) respectively.</p
Effect of DNA addition in aggregation of <i>P. aeruginosa</i> PA14.
<p>The % reduction in optical density after 60 min due to aggregation of PA14 wildtype and Ξ<i>phzA-G</i> strains before and after addition of 1 Β΅g/ml of final concentration of exogenous DNA isolated from <i>P. aeruginosa</i> cultures. Error bars represents standard deviations from the mean (nβ=β3). Asterisks indicate statistically significant (p<0.05) differences in % of aggregation in comparison to DNase I treated wildtype strain.</p
Interfacial free energy of aggregation of <i>P. aeruginosa</i> PA14 strains.
<p>(A to C) Components: Lifshitz-Van der Waals (LW ΞG) and acid-base (AB ΞG) and total interfacial free energy (Total ΞG) of aggregation of PA14 strains before and after DNase I treatment. Error bars represents standard deviations from the mean (nβ=β3). Asterisks and hash indicate statistically significant (p<0.05) differences in the free energy of aggregation in comparison to DNase I treated wildtype and Ξ<i>phzA-G</i> strain regardless of DNase I treatment respectively.</p
Pyocyanin binding with DNA responsible for thick slimy EPS formation.
<p>Photographic image showing formation of bacterial pellet and thick slimy eDNA constituted EPS (only on wildtype strain) formation after harvesting and centrifugation of 3 day old planktonic culture of <i>P. aeruginosa</i> PA14 strains (above panel). The slimy eDNA constituted EPS degraded completely after DNase I treatment in wildtype strain where as the pyocyanin deficient mutant Ξ<i>phzA-G</i> does not show any effect on DNase I treatment (below panel).</p