Reporter gene analysis of DRB1 promoter VDRE.

<p>Raji B cells were transiently transfected with pGL3 luciferase constructs as indicated together with pRL_TK to normalise luciferase activity. Open bars indicate resting cells, grey shaded bars results following stimulation of transfected cells with 1,25-dihydroxyvitamin D3. Mean+/−SD of three independent transient transfection experiments are shown, each performed in quadruplicate.</p

VDR is recruited to <i>HLA-DRB1*15</i> VDRE in PGF cells.

<p>Chromatin immunoprecipitation experiment using PGF cells either unstimulated (○) or after stimulation with 1,25-dihydroxyvitamin D3 (•). Input controls are shown (lanes 1 and 2), mock antibody immunoprecipitated controls (lanes 3 and 4) and VDR primary antibody immunoprecipitated DNA (lanes 5 and 6).</p

HLA-DRB1 promoter.

<p>Sequence shown is that for <i>HLA-DRB1*15</i>. Important regulatory elements (S, X and Y Boxes) are highlighted.</p

In vitro binding of VDR protein to the <i>HLA-DRB1*15</i> VDRE.

<p>Electrophoretic mobility shift assay showing binding of recombinant VDR and retinoic acid receptor beta (RXR) to radiolabelled oligoduplex probe corresponding to the VDRE in the proximal <i>HLA-DRB1</i> promoter region for the <i>HLA-DRB*15</i> haplotype. Two specific complexes are indicated, denoted I and II, together with a supershifted complex shown by an * symbol in the presence of antibody to VDR.</p