Depression, Anxiety and Stress Reduction in Medical Education: Humor as an Intervention

Background: In recent years there has been a growing appreciation of the issues of quality of life and stresses involved in medical training as this may affect their learning and academic performance. Objective of the study was to explore the effectiveness of humor when used as intervention in large group teaching over negative emotions amongst students. Method: The present Interventional, Randomized control trial study was carried out on medical students of 4th Semester of RMCH, Bareilly, which has total 90 students. Using simple random sampling lottery method the whole class was divided in two groups-A and B consisting of 45 students each. Group A as control group and Group B experimental group. In first and last lecture of both groups Dass-21 was used as measuring scale, for depression, anxiety and stress and results were compared to see the effect of humor on these three negative emotions. Result: Comparison of Severe and Extremely severe Stress: In Group A 40.54% in class -1 increased to 47.54% in class- 4, while in group B initial 13.15 % was reduced to 0 % (highly significant). Anxiety: In group A, after Class 1 -57.45% increased to 61.11% after class 4, while in group B, after class 1- 23.68% reduced to 2.27% only (highly significant). Depression: In group A, after Class 1 - 40.53% & 41.66 % after class 4 (not significant), while in group B, after class 1- 18.41% reduced to 0% (highly significant). Conclusion: In present study humor was found to be very effective intervention in relieving students on their negative emotions of depression, anxiety and stress to a larger extent. Further research would justify the use of humor as an effective teaching aid in medical education

Traditional healers in an age of the pharmacy and Covid-19:the case of Tharu Guruwa in Nepal

The guruwa have been a central part of Dangaura Tharu communities for many years, with various edicts referring to the role as far back as 1807. Within the existing literature, their role was conventionally defined as faith healers/shamans/Tharu cultural leaders. However, with the increasing influence of ‘western’ medicine as embodied by the exponential growth of pharmacies across Nepal, the role of the guruwa in Dangaura Tharu communities has evolved. In this article, we draw on several data sources including PhD fieldwork and subsequent research in a kamaiya basti in Kailali District, Nepal. Additionally, several interviews were conducted with guruwa in several Districts in 2020, to understand the ways that the guruwa are responding to Covid-19. It emerges that the Covid-19 pandemic constitutes a challenge as well as an opportunity to place the stature of the guruwa in Dangaura Tharu communities. Through analyzing the changes to the role of the guruwa, we consider the ways in which interactions with modernity are experienced and given meaning within Dangaura Tharu communities. We also explore the ways in which local modernities are shaped by specific histories and [Tharu] cultural practices. Finally, we consider what the future might look like for the guruwa in Dangaura Tharu communities, and how this critical role in the lives of many Dangaura Tharu communities might further adapt and evolve in the future. Ultimately, we illustrate that the role of the guruwa is at once both ‘traditional’ and ‘modern’

Phenotypic characterization and diversity of Nepalese garlic (Allium sativum L.) landraces

A total 37 garlic landraces collection from Nepal Genebank were characterized using phenotypic trait maps or agro morphological markers at National Agriculture Genetic Resources Centre (Genebank), Khumaltar, Lalitpur, Nepal during 2017 using quantitative and qualitative traits. Shannon and Weaver diversity index (H’) analysis revealed significant intra landrace diversity for both quantitative and qualitative traits. Nine principal components contributed 100 % to the cumulative variance and the first three principle components with Eigen value >1 accounted for 69.9 % of the entire variability.The cluster analysis grouped the 37 accessions into five distinct group. The Accession CO4816 from cluster 5 were superior in terms of yield attributing characters such as number of cloves/bulb, weight of cloves and yield but found to be shorter is plant height.  Pearson correlation analysis among eight quantitative traits showed highly significant positive correlation between leaf length and leaf width, leaf length and shaft length, leaf length and yield, weight of cloves and yield. The high yield is associated with leaf length, leaf width, weight of cloves and number of cloves. The frequency distribution for disease (Cercospora leafspot and Alternaria porii) occurrence were very low in 78 % of the accessions

Differential biomass allocation to plant organs and their allelopathic impact on the growth of crop plants: A case study on the invasibility of Ageratum conyzoides in Indian dry tropics

Variation in biomass allocation strategy of the alien winter annual billy goat weed (Ageratum conyzoides L.) was investigated for its invasiveness at two contrasting sites of occurrence in Indian dry tropical peri-urban region at longterm and recurrently infested site (LTI) and short-term infested (STI) site. Growth retardation impact of leaf, stem, root and reproductive part of this weed on maize and mung growths was also investigated. Compared to LTI site, the plants at STI site had higher mean basal diameter, leaf number, total plant biomass, leaf and reproductive mass fractions. Phenotypic plasticity was also higher here, albeit marginally. On the other hand, LTI plants had higher mean shoot length, belowground: aboveground biomass ratio, stem and root mass fractions. Biomass allocation to different components varied with ontogeny and site/soil conditions. While stem allocation increased with plant size at both sites, the leaf allocation generally declined. At any ontogenetic point, the reproductive and leaf allocations by plants were higher at STI compared to LTI. Root allocation at STI increased with plant size, whereas it decreased at LTI. Soil organic carbon significantly improved with increasing amount of residue of leaf, stem, root and reproductive part incorporated in soil. The leaf and root residue-amended soils recorded increasing growth retardation with increase in the amount of their incorporation in the soil

Effects of organic manures on yield and yield attributes of coffee (Coffea arabica L.) genotypes

An experiment was carried out to determine the effects of organic sources of fertilizers on yield and yield parameters of five coffee (Coffea arabica) genotypes at Gulmi district in 2015 to 2021. Five different source of organic manures (mustard cake @ 2 kg/plant, poultry manure @ 2.5 kg/plant, vermi compost @ 3 kg/plant, goat manure @ 4 kg/plant and FYM/cattle manure @ 5 kg/plant) was applied on five coffee genotypes (Chhetradip Local, Gulmi Local, Selection-10, Syangja Special and Yellow Cattura). Field experiment was laid out in factorial randomized complete block design with three replications. Irrespective of genotypes, the length of cherry was found maximum (15.3 mm) in mustard cake applied plants which was followed by poultry manure applied plants (15.2 mm). The average cherry length was found longest (15.3 mm) in Yellow Cattura and Syangja Special which was followed by genotype Chhetradip Local (15.1 mm). The highest diameter of cherry (12.7 mm) was obtained with the use of mustard cake followed by poultry manure (12.4 mm). The application of mustard cake produced the highest cherry yield (3.55 kg/plant) followed by poultry manure (3.43 kg/plant). Yellow Cattura produced the highest fresh cherry yield (3.34 kg/ha) followed by Selection-10 (3.07 kg/plant). Results indicate that poultry manure and Yellow Cattura are most effective for higher production of coffee

Detection of Methicillin Resistant Staphylococcus aureus and Determination of Minimum Inhibitory Concentration of Vancomycin for Staphylococcus aureus Isolated from Pus/Wound Swab Samples of the Patients Attending a Tertiary Care Hospital in Kathmandu, Ne

The present study was conducted to evaluate the performance of cefoxitin disc diffusion method and oxacillin broth microdilution method for detection of methicillin resistant S. aureus (MRSA), taking presence of mecA gene as reference. In addition, inducible clindamycin resistance and beta-lactamase production were studied and minimum inhibitory concentration (MIC) of vancomycin for S. aureus isolates was determined. A total of 711 nonrepeated pus/wound swab samples from different anatomic locations were included in the study. The Staphylococcus aureus was identified on the basis of colony morphology, Gram's stain, and biochemical tests. A total of 110 (15.47%) S. aureus isolates were recovered, of which 39 (35.50%) isolates were identified as MRSA by cefoxitin disc diffusion method. By oxacillin broth microdilution method, 31.82% of the Staphylococcus aureus isolates were found to be MRSA. However, mecA gene was present in only 29.1% of the isolates. Further, beta-lactamase production was observed in 71.82% of the isolates, while inducible clindamycin resistance was found in 10% of S. aureus isolates. The MIC value of vancomycin for S. aureus ranged from 0.016 g/mL to 1 g/mL. On the basis of the absolute sensitivity (100%), both phenotypic methods could be employed for routine diagnosis of MRSA in clinical microbiology laboratory; however cefoxitin disc diffusion could be preferred over MIC method considering time and labour factor

New structural analogues of curcumin exhibit potent growth suppressive activity in human colorectal carcinoma cells

<p>Abstract</p> <p>Background</p> <p>Colorectal carcinoma is one of the major causes of morbidity and mortality in the Western World. Novel therapeutic approaches are needed for colorectal carcinoma. Curcumin, the active component and yellow pigment of turmeric, has been reported to have several anti-cancer activities including anti-proliferation, anti-invasion, and anti-angiogenesis. Clinical trials have suggested that curcumin may serve as a potential preventive or therapeutic agent for colorectal cancer.</p> <p>Methods</p> <p>We compared the inhibitory effects of curcumin and novel structural analogues, GO-Y030, FLLL-11, and FLLL-12, in three independent human colorectal cancer cell lines, SW480, HT-29, and HCT116. MTT cell viability assay was used to examine the cell viability/proliferation and western blots were used to determine the level of PARP cleavages. Half-Maximal inhibitory concentrations (IC₅₀) were calculated using Sigma Plot 9.0 software.</p> <p>Results</p> <p>Curcumin inhibited cell viability in all three of the human colorectal cancer cell lines studied with IC₅₀values ranging between 10.26 μM and 13.31 μM. GO-Y030, FLLL-11, and FLLL-12 were more potent than curcumin in the inhibition of cell viability in these three human colorectal cancer cell lines with IC₅₀values ranging between 0.51 μM and 4.48 μM. In addition, FLLL-11 and FLLL-12 exhibit low toxicity to WI-38 normal human lung fibroblasts with an IC-50 value greater than 1,000 μM. GO-Y030, FLLL-11, and FLLL-12 are also more potent than curcumin in the induction of apoptosis, as evidenced by cleaved PARP and cleaved caspase-3 in all three human colorectal cancer cell lines studied.</p> <p>Conclusion</p> <p>The results indicate that the three curcumin analogues studied exhibit more potent inhibitory activity than curcumin in human colorectal cancer cells. Thus, they may have translational potential as chemopreventive or therapeutic agents for colorectal carcinoma.</p