Determination of fluorescence intensity of kidney chips.

<p>The fluorescence intensity of the UW group at 6 h after preservation was significantly higher than that of the ETK group (* <i>p</i><0.05). However, the fluorescence intensity of both groups was approximately equal at 24 h. (# <i>p</i><0.05 vs. ETK group).</p

Impact of each preservation solution and temperature on tissue TAN and EC of preserved rat kidney.

<p>(A): The TAN of fresh kidneys at 37°C and CD1h at all temperatures were lower than that of fresh kidneys at 4°C and 23°C (* <i>p</i><0.05, fresh37 vs. fresh4 in UW; # <i>p</i><0.05, fresh37 vs. fresh4 and 23 in ETK). (B) The EC of the fresh37, CD1h4, CD1h23, and CD1h37 groups was not higher than that of the fresh4 and fresh23 groups. The EC of fresh23 with UW preservation was significantly lower than that of fresh4 (* <i>p</i><0.05).</p

Survival rate, blood urea nitrogen (BUN) and serum creatinine (Cre) levels in experiment 1.

<p>(A): Survival rate of each group. Survival rate of the CD1h group was significantly greater than that of the 2-h post-CD group (* <i>p</i><0.01 using Log-Rank test.). (B): BUN. (C): Cre. BUN and Cre levels of all surviving rats were gradually recovered to a normal range by day 14 after RTx.</p

Compositions of ETK, ITK, UW or LR.

<p>Compositions of ETK, ITK, UW or LR.</p

Differentiation of survival time under each preservation condition.

*<p>vs. CD1h <i>p</i><0.01 using Log-Rank test.</p>#<p>vs. ETK23 <i>p</i><0.01 using Log-Rank test.</p>##<p>vs. ETK23 <i>p</i><0.05 using Log-Rank test.</p>†<p>vs. ITK23 <i>p</i><0.01 using Log-Rank test.</p><p>ETK, ET-Kyoto solution; UW, University of Wisconsin solution; LR, lactate Ringer's solution; ITK, IT-Kyoto solution.</p

Pathological status of kidney graft after kidney transplantation (RTx).

<p>After euthanasia on day 14 after RTx, kidneys were harvested and stained with azan stain for parenchymal fibrosis. Fibrosis was graded numerically as follows; (A): 0 = none, (B): 1 = minimal, (C): 2 = moderate, and (D): 3 = severe. In each slide, 20 fields were evaluated by four observers blinded to treatment (4×20 fields). Fibrosis of the group receiving kidneys preserved with ETK23 tended to be lower than that of the other groups, but the difference was not significant (<i>p</i>>0.05).</p

Survival rate, blood urea nitrogen (BUN) and serum creatinine (Cre) levels in experiment 2.

<p>(A): Survival rate of each group. Survival rate of the group receiving kidneys preserved with ET-Kyoto solution (ETK) for 1 h at 23°C (ETK23 group) was significantly longer than that of the other groups (# <i>p</i><0.01 using Log-Rank test.). (B): BUN and (B'): Cre of the ETK23 group. (C): BUN and (C'): Cre of the other groups without EK23. In all groups in experiment 2, BUN and Cre of all surviving rats reached a peak at day 2 and recovered to a nearly normal range by day 14. (BUN and Cre of the LR23 group was n = 1 after day 4.)</p

Remnant liver volume changes.

<p>The liver regeneration rate is expressed as remnant liver weight/estimated whole liver weight.</p

Decrease of luciferase activity.

<p>The expression level of luciferase was postoperatively observed using a noninvasive living image acquisition IVIS system. Accumulation of MSCs in the remnant liver. <b>A</b>. Most of the MSCs became trapped in the remnant liver. Thereafter, the luciferase activity diminished with time. <b>B</b>. The largest level of luciferase was 187272±119507 photons/sec/cm²/sr (sr = units of solid angle or steradian). (24 h vs. 168 h: <i>P</i><0.03; 24 h vs. 120 h: P<0.05; 72 h vs. 168 h: P<0.05).</p

Histopathological changes and Suzuki Score.

<p>Hematoxylin eosin staining of I/R-injured liver sections from the MSC group (<b>A</b>. left panel) and the Control group (<b>B</b>. right panel). <b>C</b>. The lower levels of congestion, vacuolization, and necrosis were seen in the MSC group. Suzuki scores = (MSC group vs. Control group: 1.4±1.3 vs. 3.9±1.7, <i>p</i><0.03).</p