Effect of NPC1 knockdown and anti-TNF treatment on the number of foamy macrophages and hepatocytes.

<p>Quantification of foamy macrophages (A) and hepatocytes (B) in H&E stained liver sections from mice injected for 9 weeks with mismatched (MM) and NPC1-specific ASOs and subjected to treatment (TX) with saline or anti-TNF. Each bar represents the mean ± SD number of cells per field from 3 animals in each treatment group. The lettering (a, b) shows statistically dissimilar groups.</p

Effect of NPC1 knockdown and anti-TNF treatment on hepatic apoptosis.

<p>Liver sections from mice injected for 9 weeks with mismatched (MM) and NPC1-specific ASOs and subjected to treatment (TX) with saline or anti-TNF were subjected to fluorometric TUNEL staining. Apoptotic nuclei were quantified. Each bar represents the mean ± SD number of cells per field from 5 animals in each treatment group. The lettering (a, b) shows statistically dissimilar groups. Samples with two letters represent values that are intermediate to the statistical groups and are thus not considered significantly different from either group.</p

Hepatic formation and clustering of foamy macrophages in NPC1 knockdown mice.

<p>Masson's trichrome stained liver sections from mice injected for 9 weeks with mismatched (MM) and NPC1-specific ASOs and subjected to treatment with saline or anti-TNF. Images were photographed at 200X magnification.</p

Stellate cell activation after NPC1 knockdown and anti-TNF treatment.

<p>Liver sections from mice injected for 9 weeks with mismatched (MM) and NPC1-specific ASOs and subjected to treatment with saline or anti-TNF were subjected to α-smooth muscle actin immunohistochemistry (A). Immunohistochemical reaction product was quantified (B). Each bar represents the mean± SD relative area of brown reaction product per field from 5 animals in each treatment group. The lettering (a, b) shows statistically dissimilar groups. Samples with two letters represent values that are intermediate to the statistical groups and are thus not considered significantly different from either group.</p