Female flower frequency and GTFL-1 genotype in F₂ population.

<p>For 160 F₂ plants from OHB61-5x OHB95-1A, the frequency of female flowers and GTFL-1 genotype in each plant was investigated, and the scoring results are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0087138#pone.0087138.s008" target="_blank">Table S5</a>. The average frequency of female flowers (%) is indicated in the plants for each GTFL-1-genotype: “A” for the homozygotes of the OHB95-1A-type, “G” for the homozygotes of the OHB61-5-type and heterozygotes.</p

Genetic map of putative gynoecious locus (<i>Mcgy</i>).

<p>The genetic distances and location between each RAD-tag marker and the <i>Mcgy</i> locus were calculated from the genotypes of 55 gynoecious F₂ plants. The sequences of alleles in these markers are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0087138#pone-0087138-t004" target="_blank">Table 4</a> (GTFL-1, GTFL-2, GTFL-3, GTFL-11, GTFL-13) and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0087138#pone.0087138.s005" target="_blank">Table S2</a> (GT1998).</p

Segregation of gynoecy and monoecy in F₂ population from OHB61-5x OHB95-1A.

<p>Segregation of gynoecy and monoecy in F₂ population from OHB61-5x OHB95-1A.</p

Summary of RAD-seq analysis of parental lines and bulked F₂ samples using AseI.

<p>Summary of RAD-seq analysis of parental lines and bulked F₂ samples using AseI.</p

RAD-tag markers using AseI, showing linkage to gynoecy in <i>M.charantia</i>.

*<p>SNPs between parental lines in each marker were underlined in the tag sequences.</p>**<p>SNP between OHB61-5 and OHB95-1A in each locus was indicated.</p