Are all eggs equal? : embryonic development and nutrient metabolism in chicken eggs of different origins

Hatching eggs, supplied to hatcheries are originating from different origins varying in breed, strain, and breeder age. These hatching eggs can be different in size, composition and eggshell properties, which might influence nutrient and O2 availability and consequently could affect embryonic development and nutrient metabolism. The aim of this thesis was therefore 1) to investigate effects of egg origin on nutrient and O2 availability, 2) to investigate effects of egg origins on nutrient metabolism and embryonic development and 3) to investigate consequences of different egg origins on the incubation process and hatching characteristics. In five studies, effects of different egg origins on nutrient and O2 availability, nutrient metabolism, embryo development and hatching characteristics were investigated. The first and second study focused on breeder age and egg size. The third study on breed; broilers and layers. The fourth study on broiler strain and the fifth study on breeder age, strain and eggshell temperature (EST). The overall findings in this thesis suggest that hatching eggs from different origins are not equal in availability of nutrients and O2. Nutrient availability is altered through variation in yolk size, especially by the effects of breeder age and breed. O2 availability is altered by differences in eggshell properties, which is influenced by especially breed and broiler strain. The availability of both nutrients and O2 plays a role on nutrient metabolism measured as embryonic heat production (HP) and consequently on embryonic development. Between incubation day (E) E7 and E14, both nutrient and O2 availability might affect nutrient metabolism as shown in the results of the broiler and layer comparison. Between E14 and hatching, the availability of O2 becomes the most determinant factor for nutrient metabolism and consequently for embryonic development. An increase in EST from 37.8 to 38.9°C from E7 onward resulted in an acceleration of nutrient metabolism and embryonic development until E16, but thereafter a high EST resulted in reduced yolk free body mass development. Embryos with an accelerated metabolic speed at an early stage of incubation, caused by an increased EST, might reach limited O2 availability at a higher magnitude than the embryos at a normal EST. As a result, nutrient metabolism is restricted and embryonic development is depressed. It can be concluded that not only the HP, but also the availability of O2 is crucial to be taken into account for developing incubator temperature. The principle is to obtain an optimal EST, which could maintain the balance between O2 requirement (driven by nutrient metabolism) and O2 availability for a continuing optimal nutrient metabolism to generate sufficient energy for embryonic development throughout incubation

Energy utilization and heat production of embryos from eggs originating from young and old broiler breeder flocks

Two experiments were conducted to study the interaction between breeder age and egg size on the energy utilization (experiment 1) and heat production (experiment 2) of broiler embryos. In experiment 1, a total of 4,800 Ross-308 hatching eggs from 2 breeder ages (29 and 53 wk of age, or young and old) and, within each age, 2 egg sizes (57 to 61 g and 66 to 70 g, or small and large) were used. In experiment 2, a total of 240 Ross-308 hatching eggs from 2 breeder flocks at 29 (young) and 53 (old) wk of age, and which were selected from the same egg weight range (58 to 61 g), were tested in 2 replicate chambers. In experiment 1, it was shown that the amount of yolk relative to albumen was higher in the old flock eggs, and this effect was more pronounced in the large eggs. The old flock eggs, especially the larger egg size, contained more energy as a result of a greater yolk size. Energy utilization of the embryos was positively related to yolk size and the amount of energy transferred to yolk-free body (YFB) was largely determined by the available egg energy. The efficiency of converting egg energy into chick body energy (EYFB) was equal for both egg sizes and both breeder age groups. Chick YFB weight of young and old flock eggs was equal. However, dry YFB weight of chicks from old flock eggs was higher than in chicks from young flock eggs, which was associated with more protein and fat content and thus more energy accumulated into YFB. As a consequence, embryos derived from old flock eggs produced more heat from d 16 of incubation onward than those of the young flock eggs. In conclusion, the higher energy deposition into chick YFB of old flock eggs, leading to higher embryonic heat production, is the result of a higher amount of available energy in the egg and is not due to changes in EYFB

Development and nutrient metabolism of embryos from two modern broiler strains

A progressive selection for broiler live and processing performance traits has changed broiler growth patterns during the post hatch period. However, limited information is available to understand whether changes have also occurred during the embryonic stages. This study aims to examine influences of broiler strain on nutrient availability, embryonic development, and nutrient metabolism during incubation. Hatching eggs of Ross 308 and Cobb 500 fast feathering were selected from breeder flocks aged 43 to 46 weeks at an egg weight range of 60 to 63 g. Eggs were obtained in 2 batches, 120 eggs per strain per batch. For each batch, 20 eggs per strain were used to determine egg composition and nutrient availability. The remaining eggs were incubated separately in one of 2 climate respiration chambers at an eggshell temperature of 37.8°C. The results showed that Ross 308 eggs had a higher yolk:albumen ratio with 0.9 g more yolk and 0.7 g less albumen than Cobb 500. Albumen + yolk of Ross 308 eggs had a higher dry matter (¿ = 0.24 g) and crude fat (¿ = 0.23 g) than that of Cobb 500 eggs, but a similar amount of crude protein. Albumen and yolk of Ross 308 eggs had a higher energy content (¿ = 8.9 kJ) compared to Cobb 500 eggs. At 3¿h after hatch, Ross 308 chicks were 0.2 cm longer and had a 0.6 g heavier yolk free body mass (YFBM) than Cobb 500 chicks. During incubation, Ross 308 embryos used 13.9 kJ more energy than Cobb 500, and the efficiency of converting energy used to YFBM (EYFB) was approximately 7.6% lower compared to Cobb 500. Ross 308 chicks hatched approximately 4¿h later and had less hepatic glycogen (¿ = 5 mg) than Cobb 500 chicks. It can be concluded that, Cobb 500 and Ross 308 differ in egg nutrient availability and have different trajectories for embryonic development and nutrient metabolism during incubation