Pregnenolona administrado en núcleo septal lateral modifica la adquisición de la memoria en un modelo de ratas macho

Pregnenolona (Preg) y Preg-sulfato (Preg-S) son los esteroides neuroactivos  (EN)  que tienen  un  papel  como moduladores de la memoria, entre otras funciones.

Pregnanolona sulfato modula positivamente diferentes sistemas de neurotransmisión hipotalámicos involucrados en el control de la liberación de LH en la rata hembra

Concluimos que Preg-S modifica la secreción de LH mediante la modulación de la funcionalidad de receptores GABAA, inhibiendo su secreción, y mediante los α1 y α2 estimulando su liberación, así como también sobre el sistema de receptores glutamatérgicos no NMDA, sin modificar la funcionalidad de los GABAB, lo cual propone a este neuroesteroide como activo modulador multisistémico neuroendócrino sobre el sistema reproductivo en la rata hembra

The novel object recognition memory: neurobiology, test procedure, and its modifications

Animal models of memory have been considered as the subject of many scientific publications at least since the beginning of the twentieth century. In humans, memory is often accessed through spoken or written language, while in animals, cognitive functions must be accessed through different kind of behaviors in many specific, experimental models of memory and learning. Among them, the novel object recognition test can be evaluated by the differences in the exploration time of novel and familiar objects. Its application is not limited to a field of research and enables that various issues can be studied, such as the memory and learning, the preference for novelty, the influence of different brain regions in the process of recognition, and even the study of different drugs and their effects. This paper describes the novel object recognition paradigms in animals, as a valuable measure of cognition. The purpose of this work was to review the neurobiology and methodological modifications of the test commonly used in behavioral pharmacology

Experimental Models of Neurodegenerative Diseases: A Simple Method to Check Both Viability of Neural Tissue and Cannulae Location After Stereotaxic Surgeries

The reagent MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a yellow tetrazole) is reduced to formazan in living cells, giving a purple colored product. The product absorbance issusceptible to be measured at a certain wavelength by a spectrophotometer. We report in this paper the use of MTT as a suitable method for checking both the viability of neural tissues as well as the correct location of stereotaxically placed cerebral cannulae. Evidence is given that this method can be useful not only because it is a new way of utilizing a vital staining to evaluate cannulae location, but also because MTT is a simple, effective and cheaper way of assessing the arousal level of neural tissues under study. In doing so, we can effectively test the stimulatory and/or inhibitory actions of several reagents in what we finally named as MTT-micro assay on living tissues: the MTT-malt.Fil: García, S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Nanfaro, F.. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Casas, Sebastián Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Cabrera Kreiker, Ricardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Yunes, Roberto Miguel Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentin