14 research outputs found
Molecular Scanning and Morpho-Physiological Dissection of Component Mechanism in Lens Species in Response to Aluminium Stress.
Aluminium (Al) stress was imposed on 285 lentil genotypes at seedling stage under hydroponics to study its effects on morpho-physiological traits where resistant cultigens and wilds showed minimum reduction in root and shoot length and maximum root re-growth (RRG) after staining. Molecular assortment based on 46 simple sequence repeat (SSR) markers clustered the genotypes into 11 groups, where wilds were separated from the cultigens. Genetic diversity and polymorphism information content (PIC) varied between 0.148-0.775 and 0.140-0.739, respectively. Breeding lines which were found to be most resistant (L-7903, L-4602); sensitive cultivars (BM-4, L-4147) and wilds ILWL-185 (resistant), ILWL-436 (sensitive) were grouped into different clusters. These genotypes were also separated on the basis of population structure and Jaccard's similarity index and analysed to study Al resistance mechanism through determination of different attributes like localization of Al and callose, lipid peroxidation, secretion of organic acids and production of antioxidant enzymes. In contrast to sensitive genotypes, in resistant ones most of the Al was localized in the epidermal cells, where its movement to apoplastic region was restricted due to release of citrate and malate. Under acidic field conditions, resistant genotypes produced maximum seed yield/plant as compared to sensitive genotypes at two different locations i.e. Imphal, Manipur, India and Basar, Arunanchal Pradesh, India during 2012-13, 2013-14 and 2014-15. These findings suggest that Al stress adaptation in lentil is through exclusion mechanism and hybridization between the contrasting genotypes from distinct clusters can help in development of resistant varieties
Genotypes with different origins, type and sensitivity to Al stress (AR).
<p>Genotypes with different origins, type and sensitivity to Al stress (AR).</p
Antioxidant enzyme activity of A- SOD, B- CAT, C- APX and D-GPX in four lentil genotypes: Resistant breeding lines (L-7903, L-4602), Sensitive cultivars (BM-4, L-4147) and two wild accessions: Resistant (ILWL-185), Sensitive (ILWL-436) under Al stress (74 and 148μM Al) conditions after 48 h exposure.
<p>Means with the same small letters for each part of the plant do not statistically differ by the Tukey test at P≤0.05.</p
Seed yield of lentil genotypes grown under low pH condition at Imphal, Manipur, India (pH 4.8) and Basar, Arunachal Pradesh, India (pH 5.1) during 2012–13, 2013–14 and 2014–15.
<p>Data shown are mean ± SEm. Bars that do not share common letters are significantly different by Duncan’s post hoc test at P<0.05.</p
Al contents in root and shoot of lentil: resistant breeding lines (L-7903, L-4602), sensitive cultivars (BM-4, L-4147) and resistant wild accession (ILWL-185), sensitive wild accession (ILWL-436), after exposure to two Al concentrations (0, 74 and 148 μM) for 48 h duration.
<p>Mean values with same small letters for each part of the plant do not statistically differ by the Tukey test at P≤0.05.</p
Root tip of lentil seedling showing callose accumulation under control, 74μM and 148 μM Al concentrations.
<p>Bar in each figure represents 1 mm.</p
Model based population structure plot with K = 3, using Structure with 46 SSR markers.
<p>Colour codes: red = population I (wild accessions), green = population II (cultigens) and blue = population III (‘ILL’ series of cultigens).</p
Allelic variations and PIC values for SSR markers identified in 285 lentil genotypes.
<p>Allelic variations and PIC values for SSR markers identified in 285 lentil genotypes.</p