Bis(4-fluoro­benz­yl)bis­(4-phenyl-5-sulfanyl­idene-4,5-dihydro-1,3,4-thio­diazole-2-thiol­ato)tin(IV)

In the title complex, [Sn(C7H6F)2(C8H5N2S3)2], including the weak Sn—N inter­actions, the SnIV atom is situated in a distorted trans-octa­hedral geometry, and the equatorial plane is defined by two chelating 4-phenyl-5-sulfanyl­idene-4,5-dihydro-1,3,4-thio­diazole-2-thiol­ate ligands. The apical positions are occupied by two C atoms of 4-fluoro­benzyl groups

Bis(chloro­acetato-κ2 O,O′)bis­(2-fluoro­benzyl-κC 1)tin(IV)

In the title complex, [Sn(C2H2ClO2)2(C7H6F)2], the SnIV atom is located on a twofold rotation axis and forms a strongly distorted trans-octa­hedral geometry. The equatorial plane is defined by two chelating chloro­acetate ligands with asymmetrical Sn—O bond lengths, while the axial positions are occupied by the C atoms of two 2-fluoro­benzyl groups. In the crystal, infinite chains in the [010] direction are formed through inter­molecular Sn⋯O inter­actions [Sn⋯O separation = 3.682 (3) Å]

catena-Poly[[[aqua­[2-(6-chloro­pyridin-3-yl)acetato-κO]sodium]-di-μ-aqua] monohydrate]

The crystal structure of the title compound, {[Na(C7H5ClNO2)(H2O)3]·H2O}n, features polymeric chains along [010]. The Na+ cation is octa­hedrally coordinated by four bridging water mol­ecules, a terminal water mol­ecule and an O atom derived from a monodentate carboxyl­ate ligand. Adjacent polyhedra share two O⋯O edges. The polymeric chains are linked into a three-dimensional network via O—H⋯O and O—H⋯N hydrogen bonds

Implementing a new fully stepwise decomposition-based sampling technique for the hybrid water level forecasting model in real-world application

Various time variant non-stationary signals need to be pre-processed properly in hydrological time series forecasting in real world, for example, predictions of water level. Decomposition method is a good candidate and widely used in such a pre-processing problem. However, decomposition methods with an inappropriate sampling technique may introduce future data which is not available in practical applications, and result in incorrect decomposition-based forecasting models. In this work, a novel Fully Stepwise Decomposition-Based (FSDB) sampling technique is well designed for the decomposition-based forecasting model, strictly avoiding introducing future information. This sampling technique with decomposition methods, such as Variational Mode Decomposition (VMD) and Singular spectrum analysis (SSA), is applied to predict water level time series in three different stations of Guoyang and Chaohu basins in China. Results of VMD-based hybrid model using FSDB sampling technique show that Nash-Sutcliffe Efficiency (NSE) coefficient is increased by 6.4%, 28.8% and 7.0% in three stations respectively, compared with those obtained from the currently most advanced sampling technique. In the meantime, for series of SSA-based experiments, NSE is increased by 3.2%, 3.1% and 1.1% respectively. We conclude that the newly developed FSDB sampling technique can be used to enhance the performance of decomposition-based hybrid model in water level time series forecasting in real world

Hydrochemistry and Characteristics of Groundwater: Case Study Water Contamination at Citarum River Upstream

Rancaekek and Sayang area, West Java, are the area where many industrial factories are located.Thus, the region becomes the targeted destination for industrial development.The  population in the area is rising due to the growth of industries causing the regional development becomes uncontrollable. In addition, the constant increment of waste and also poor-coordinated disposal systems may result in groundwater contamination in the areas. The rapid growth of the area increase the need for groundwater as well as the need for more research about contamination at Rancaekek and Sayang. The research aims to explore the spread of groundwater contamination in the area. The research method is carried out based on the analysis of Geological Mapping, Hydrogeological Mapping and chemical characteristics of the groundwater in the area. Chemical analyses of the groundwater were conducted through laboratory test of groundwater samples at specific spots of dug wells. The lab test results were further analyzed to determine the contamination zone. The findings reveal that the distribution of contamination in the area follow the shallow ground water flow patterns, the water contamination contains heavy metal and there is degradation of soil fertility. The findings suggest the stakeholders to delineate the contaminated area, and increase the dissemination of environmental awareness

Association of TRB3 Q84R polymorphism with polycystic ovary syndrome in Chinese women

<p>Abstract</p> <p>Background</p> <p>Tribbles 3 (TRB3) affects insulin signalling by inhibiting insulin-stimulated Akt phosphorylation and subsequent activation. A single nucleotide polymorphism located in the second extron of the human TRB3 gene is thought to be associated with insulin resistance. The latter is a core abnormality in PCOS independent of obesity. The present study was designed to clarify the relationships of TRB3 Q84R polymorphism with PCOS in a Chinese women group.</p> <p>Methods</p> <p>A case-control study with two groups: PCOS group (n = 336) and control group of infertility women for tubal and/or male factor (n = 116) was performed. Genotyping of the TRB3 R84 variant was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).</p> <p>Results</p> <p>The frequency of genotype QQ in PCOS women was significantly lower, while genotype QR and RR were significantly higher than that in control group (p < 0.05). However, the difference disappeared after adjustment for BMI. At glucose1h, glucose2h and insulin2h point, the difference between QQ individuals and R84 allele carriers in PCOS women reached statistical significance during OGTT (p < 0.05).</p> <p>Conclusions</p> <p>TRB3 Q84R polymorphism is associated with obesity and especially glucose metabolism and not associated with polycystic ovary syndrome because of compositional characteristics of phenotype in Chinese PCOS women.</p

The prognostic significance of LncRNA BLACAT1 overexpression in various tumors: a meta-analysis

Objective:Recent studies have revealed increasing evidence that the long non-coding RNA bladder cancer associated transcript 1 (LncRNA BLACAT1) plays an essential role in the emergence of different malignancies. This meta-analysis aimed to evaluate the prognostic significance of LncRNA BLACAT1 in various cancers.Methods:Six electronic databases (PubMed, Embase, Medline, Web of Science, China National Knowledge Infrastructure (CNKI), and the Chinese WanFang database) were comprehensively searched for relevant studies. The analysis of overall survival (OS) and clinicopathological characteristics was conducted.Results:Nineteen studies with 1,559 patients were eventually eligible to be included in this meta-analysis. High expression level of LncRNA BLACAT1 was identified to be linked with shorter OS (HR: 2.02, 95% CI: 1.66–2.46, p &lt; 0.001) and PFS (HR: 2.424, 95% CI: 1.827–3.020, p &lt; 0.001) in cancer patients as opposed to low expression levels. Subgroup analysis showed that analysis model (multivariate or univariate), cut-off value (mean or median), sample size (more or fewer than 100), and cancer type had little effect on OS in multiple tumors. Moreover, high LncRNA BLACAT1 expression was associated with positive lymph node metastasis (HR: 2.29, 95% CI: 1.66–3.16, p &lt; 0.00001), advanced clinical stage (HR: 2.29, 95% CI: 1.65–3.19, p &lt; 0.00001) and worse differentiation status (HR: 0.58, 95% CI: 0.37–0.92, p = 0.02), compared to low LncRNA BLACAT1 expression.Conclusion:The findings highlight that high LncRNA BLACAT1 expression might be detrimental and induce a worse prognosis for cancer patients

Monitoring the conformational ensemble and lipid environment of a mechanosensitive channel under cyclodextrin-induced membrane tension

This project was supported by a Biotechnology and Biological Sciences Research Council (BBSRC) grant (BB/S018069/1) to C.P., who acknowledges support from the Wellcome Trust (WT) (219999/Z/19/Z) in the form of studentship for B.J.L. We also acknowledge support from the Chinese Scholarship Council (CSC) in the form of studentships for N.Y., Y.M., B.W., respectively. T.K.K. is supported by a Sir Henry Dale Fellowship funded by the WT and the Royal Society (223268/Z/21/Z). B.E.B. and C.P. thank the Leverhulme Trust (RPG-2018-397) for support. Funding from BBSRC (BB/R013780/1; BB/T017740/1) equipment grants enabled the purchase of the Qband Bruker pulse EPR spectrometer and University of Leeds funding the Bruker 950 MHz NMR spectrometer.Membrane forces shift the equilibria of mechanosensitive channels enabling them to convert mechanical cues into electrical signals. Molecular tools to stabilize and methods to capture their highly dynamic states are lacking. Cyclodextrins can mimic tension through the sequestering of lipids from membranes. Here we probe the conformational ensemble of MscS by EPR spectroscopy, the lipid environment with NMR, and function with electrophysiology under cyclodextrin-induced tension. We show the extent of MscS activation depends on the cyclodextrin-to-lipid ratio, and that lipids are depleted slower when MscS is present. This has implications in MscS’ activation kinetics when distinct membrane scaffolds such as nanodiscs or liposomes are used. We find MscS transits from closed to sub-conducting state(s) before it desensitizes, due to the lack of lipid availability in its vicinity required for closure. Our approach allows for monitoring tension-sensitive states in membrane proteins and screening molecules capable of inducing molecular tension in bilayers.Peer reviewe

Quantitative proteomic analysis and replacement therapy identifies haptoglobin as a therapeutic target in a murine model of SLE-associated diffuse alveolar hemorrhage

BackgroundDiffuse alveolar hemorrhage (DAH) is a catastrophic clinical syndrome and one of the manifestations of pulmonary involvement in systemic lupus erythematosus (SLE), which is characterized by hemoptysis, diffuse pulmonary infiltrates, and respiratory failure. However, the treatment options for DAH remain limited, and DAH-related studies are needed to explore more effective therapeutic directions for better disease management and improved prognosis.MethodsThis study utilized the pristane-induced DAH murine model to mimic the pathological process of DAH in patients with SLE. Proteomic analysis was conducted to detect differentially expressed proteins (DEPs) in the plasma of surviving and non-surviving mice, followed by an analysis of biological functions and pathways. The most significant DEP was then confirmed in the plasma of SLE patients with or without DAH and DAH murine model with or without fatal outcomes. Finally, the therapeutic value of haptoglobin (Hp) replacement was validated in a DAH murine model through lung histopathology, RT-qPCR, and survival analysis.ResultsThis study identified 178 DEPs, with 118 upregulated and 60 downregulated DEPs in the non-survival group. Within a set of notable Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, complement and coagulation cascades emerged as the most prominent pathway associated with the process of DAH. Later, the most significant DEP, haptoglobin (Hp), was confirmed to exhibit a significant decrease in the plasma of individuals with SLE-DAH and DAH murine model with poor outcomes by the ELISA test. Finally, compared with the control group, the severity of DAH in the Hp treatment group was alleviated significantly, as manifested by the decreased levels of pro-inflammatory cytokines (IL-6 and TNF-α), increased levels of anti-inflammatory cytokines (IL-10 and TGF-β), and decreased mortality.ConclusionA reduction in plasma Hp levels was observed in SLE-DAH, and the replacement therapy with Hp could alleviate pulmonary hemorrhage and reduce mortality in DAH mice. This study identified Hp as a potential biomarker for its clinical diagnosis and a direction for treatment

Analysis on the status quo of construction industry and workers’ professional literacy

Under the background of the new building industrialization in China, the traditional cast-in-place construction mode begins to change to assembly construction mode. Such subversive changes make all regions face various assembly construction quality problems caused by traditional workers’ lack of assembly construction knowledge, low awareness and unskilled operation. Therefore, improving workers’ professional quality is very important to improve the quality of assembly construction. Through the analysis of a large number of domestic and foreign literature, this paper found that when studying prefabricated buildings from different dimensions such as quality, cost, schedule and safety, Will involve the “operation proficiency", “education level”, “professional skills”, “vocational training”, “qualification”, “working years”, “professional experience”, “lack of knowledge”, “reputation”, “professional pride”, “mobile operation”, “work attitude” and other aspects of the workers, These factors focus on safety awareness, operation norms, vocational training, work experience, professional knowledge, cultural level, quality awareness, technical skill level. All these factors will affect the quality of assembly construction, and the quality of workers is one of the reasons for poor construction performance, frequent safety accidents and low construction quality